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Phosphotungstic acid staining method

For light microscopic examination, liver tissue was fixed in 10 % buffered formalin, embedded in paraffin, sectioned and stained with hematoxylin and eosin. In some cases, preparations were stained with PTAH (phosphotungstic acid-haematoxylin), by the Van Gieson method and the PAS (periodic acid-Schiff)... [Pg.390]

Phosphotungstic acid will precipitate many proteins, lipids and alkaloids. Many soluble basic dyestuffs will form insoluble pigments known as lakes , by complexing them with phosphomolyb-date or phosphotungstate anions. Both insolubility and improvement of light fastness are achieved (Chapter 12.8). The formation of lakes of this kind can be used as a method of detection of P (Chapter 14.1), and in staining biological specimens for electron microscopy. [Pg.299]

See other pages where Phosphotungstic acid staining method is mentioned: [Pg.63]    [Pg.25]    [Pg.146]    [Pg.294]    [Pg.110]    [Pg.315]    [Pg.3154]    [Pg.14]    [Pg.103]    [Pg.115]    [Pg.118]    [Pg.119]    [Pg.267]    [Pg.268]    [Pg.95]    [Pg.94]    [Pg.233]    [Pg.99]    [Pg.161]    [Pg.168]    [Pg.173]    [Pg.177]    [Pg.382]    [Pg.385]    [Pg.8]   


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