Hematoxylin histological staining method

The staining technique will depend on the type of cell of interest. Hematoxylin and eosin (H E) stain is the most widely used method in histology. Using this technique, basophilic white blood cells stain dark blue, eosinophilic white blood cells stain bright red, and neutrophils stain a neutral pink. 

Histologic analysis of hematoxylin and eosin stained sections of liver and heart from male PPARa -/- mice following a single injeetion of etomoxir revealed marked lipid accumulation in both organs with high fatty acid oxidative flux (Fig. 3 A). The sections were also stained with oil red O, a method for staining of neutral lipids (Fig. 3B). The livers of PPARa-/-male and female vehicle-treated eontrol mice contained patehy areas of small red droplets but not their heart, consistent with the existence of a mild... 

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