Intracellular recording

A number of studies in fact show clear Di effects. Intracellular recording from striatal neurons in rat brain slices show a cAMP-mediated Di-dependent (blocked by SCH 23390) suppression of a voltage-dependent sodium current which make the cell less responsive. 

Morales, F. R. Chase, M. H. (1978). Intracellular recording of lumbar motoneuron membrane potential during sleep and wakefulness. Exp. Neurol. 62, 821-7. 

Grace A., Bunney B. (1980). Nigral dopamine neurons intracellular recording and identification with L-dopa injection and histofluorescence. Science Wash. DC 210, 654-6. 

FIGURE 4.14 Schematic cross-section of the platform for in-vitro intracellular recording of ion concentration, designed for hepatocyte cell culture (a) and ESEM pictures of a micropipette (tilted at 75°) filled with the membrane cocktail. (From [102].)... 

Spencer NJ, Smith TK Simultaneous intracellular recordings from longitudinal and circular muscle during the peristaltic reflex in guinea-pig distal colon. J Physiol 2001 533 ... 

It is customary today to classify anti arrhythmic drugs according to their mechanism of action. This is best defined by intracellular recordings that yield monophasic action potentials. In the accompanying figure, the monophasic action potentials of (A) slow response fiber (SA node) and (B) fast Purkinje fiber are shown. For each description that follows, choose the appropriate drug with which the change in character of the monophasic action potential is likely to be associated... 

Aghajanian, G. K... and VanderMaelen, C. P. (1982) Intracellular recordings from serotonergic dorsal raphe neurons Pacemaker potentials and the effect of LSD. Brain Res., 238 463-469. 

Purves RD. Microelectrode Methods for Intracellular Recording and Iontophoresis, Academic Press, London, UK, 1981. 

By means of intracellular recording and staining methods, we have examined the responses of AL neurons to stimulation of the ipsilateral antenna with each of the sex pheromone components as well as partial and complete blends (75). In accordance with results of behavioral and sensory-receptor studies, components A and B are the most effective and potent sex pheromone components for eliciting physiological responses in the male-specific AL neurons. On the basis of these responses, we classified the neurons into two broad categories pheromone generalists and pheromone specialists (76). Pheromone generalists are neurons that respond similarly to stimulation of either the component A input channel or the component B input channel and do not respond differently when the complete, natural pheromone blend is presented to the antenna. In contrast, pheromone specialists are neurons that can discriminate between antennal stimulation with component A and stimulation with component B. There are several types of pheromone specialists. Some... 

Continuous intracellular recording of action potentials and on-line analysis of resting membrane potential, maximum rate of depolarization, upstroke amplitude and action potential duration using Notocord HEM data acquisition system. 

Cardiac Action Potential In Vitro Purkinje Fibers. Intracellular recording of action potentials from cardiac Purkinje fibers isolated from dog or sheep ventricle. Measurement of maximum rate of depolarization and action potential duration to detect sodium and potassium channel interactions, respectively, according to recommendations in EM A CPMP Points to Consider document, CPMP 986/96 (1998). 

West AR, Moore H, Grace AA. 2002. Direct examination of local regulation of membrane activity in striatal and prefrontal cortical neurons in vivo using simultaneous intracellular recording and microdialysis. J Pharmacol Exp Ther 301(3) 867-877. 

Schematic representation of the heart and normal cardiac electrical activity (intracellular recordings from areas indicated and ECG). Sinoatrial (SA) node, atrioventricular (AV) node, and Purkinje cells display pacemaker activity (phase 4 depolarization). The ECG is the body surface manifestation of the depolarization and repolarization waves of the heart. The P wave is generated by atrial depolarization, the QRS by ventricular muscle depolarization, and the T wave by ventricular repolarization. Thus, the PR interval is a measure of conduction time from atrium to ventricle, and the QRS duration indicates the time required for all of the ventricular cells to be activated (ie, the intraventricular conduction time). The QT interval reflects the duration of the ventricular action potential.

Kleber AG, Janse MJ, van Capelle FJL, Durrer D Mechanism and time course of ST and TQ segment changes during acute regional myocardial ischemia in the pig heart determined by extracellular and intracellular recordings. Circ Res 1978 42 603-613. 

Figure 30-32 Intracellular recordings from isolated neurons of the mollusc Aplysia (A) beating pacemaker, (B) bursting pacemaker, and (C) oscillating pacemaker. From Chen et a/.1002...

Intracellular recordings from taste cells of rat and hamster show that even primary receptor cells are sensitive tu three or four of the so-called basic taste modalities. Consequently, it is generally held that a variety or dilferem receptor sites commonly exist on the receptor membrane of anyone receptor cell. Biochemical characterization or events at receptor sites has progressed in analyzing electrolyte and carbuhydrate stimulation. 

Figure 2.3 Sensitivities of rods and cones of the mudpuppy (a large aquatic salamander) retina. The graph shows the intensity-response curves, which were measured intracellularly from the retina of a dissected eye. The response is shown as a fraction of the response at saturation. A fit to the data is also shown. Rods are approximately 25 times more sensitive than cones. (Reprinted with permission from Gordon L. Fain and John E. Dowling. Intracellular recordings from single rods and cones in the mudpuppy retina. Science, Vol. 180, pp. 1178-1181, June, Copyright 1973 AAAS).

Fain GL and Dowling JE 1973 Intracellular recordings from single rods and cones in the mudpuppy retina. Science 180, 1178-1181. 

Reprinted with permission from Gordon L. Fain and John E. Dowling. Intracellular recordings from single rods and cones in the mudpuppy retina. Science, Vol. 180, pp. 1178-1181, June, Copyright 1973 AAAS... 

Effects of three antiseizure drugs on sustained high-frequency firing of action potentials by cultured neurons. Intracellular recordings were made from neurons while depolarizing current pulses, approximately 0.75 s in duration, were applied (on-off step changes indicated by arrows). 

Gubitz AK, Widdowson L, Kurokawa M et al (1996) Dual signalling by the adenosine A2a receptor involves activation of both N- and P-type calcium channels by different G proteins and protein kinases in the same striatal nerve terminals. J Neurochem 67 374-81 Haas HL, Greene RW (1988) Endogenous adenosine inhibits hippocampal CA1 neurones further evidence from extra- and intracellular recording. Naunyn Schmiedeberg s Arch Pharmacol 1988 337 561-5... 

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