Blue-Stain

A test for secondary amines (e.g. proline) is the Chloranil test (1 drop of a 2% acetaldehyde solution in DMF, followed by one drop of a 2% solution of p-chloranil in DMF, leave for 5 mins). A positive test gives blue stained beads. 

Fig. 15. Effects of turbulent shear stress level and exposure time on cell viability measured by trypan blue staining. Cells were sheared in a concentric cylinder viscometer [1]...

A dual isotope labeling technique [85] has been used to measure membrane permeability in plant cells, based on the selective permeabiHty of the membranes of living cells to tritiated water and carbon-14 labeled mannitol. Kieran [29] showed that the results of the dual isotope labeling and Evan s Blue staining methods correlated well as indicators of cell viability however, the latter was preferable in terms of reagent cost and ease of analysis. 

A) Proteins were resolved by SDS-PAGE and visualized by Coomassie blue staining. Lane 1, 4 )ig purified PGl Lane 2, 2 pg purified PG2 Lane 3,2 pg purified subunit. 

Isolated hepatocytes incubated with ionic iron rapidly undergo lipid peroxidation. Some studies have not shown a consequent decrease in viability (as indicated by uptake of trypan blue or release of enzymes). This is probably a result of short incubation times, as changes in viability lag behind increases in lipid peroxidation, and may not occur for more than 2 h after lipid peroxidation begins (Bacon and Britton, 1990). Recent studies have shown strong correlations between increased lipid peroxidation [production of thiobarbituric acid (TBA) reactants] and loss of cell viability (trypan blue staining) (Bacon and Britton, 1989). The significance of the lag between lipid peroxidation and decreases in cell viability is as yet uncertain. 

Trypan Blue exclusion (TB) Dead cells blue stained Cell membrane Yes3 [34]... 

Decolorize the smear in aniline-xylene, agitating the slide gently until no purple runs from it (the use of a second Coplin jar of aniline-xylene after the majority of blue stain has been removed aids the visual assessment of decolorization). 

Fig. 13.2. The peptide components of H-gal-GP and TSBP visualized by Coomassie Blue staining of non-reducing (lanes 1 and 3) and reducing (lanes 2 and 4) SDS-PAGE gels.

The quality of RNA and transfer can be evaluated by methylene blue staining. This is done by immersing the membrane in 5% acetic acid for 5 min and then in 0.1% methylene blue in 5% acetic acid for 5 min. The membrane is then washed in water, and distinct bands of the 25S (3400 nts), 18S (1800 nts) rRNA, and RNA marker bands should appear. Mark the positions of these with a pencil because the methylene blue staining will disappear during the hybridization. 

Fig. 2 Representative histological images of (a) bovine annulus fibrosus (H E staining), (b) bovine nucleus pulposus (H E staining), and (c) porcine notochordal cells (Alcian blue staining for GAGs with fast red nuclei stain)...

B) The blue staining in the supernatant of strain MR16 becomes visible after 1 day. 

Deuchar EM. Staining sections before autoradiographic exposure excessive background graining caused by celestin blue. Stain Technol 1962 37 324. 

Fluorescence Microscopy of Aniline Blue Stained Pistils... 

The aniline blue stain penetrates macerated tissue very quickly and in a few minutes the slide is ready to be observed. Slides do not dry out for several hours. They can be stored for a longer time (about 2 wk) in a cool, dark place if sealed around the coverslip with parafilm or Vaseline. 

Plant Cells and Tissues Structure-Function Relationships. Methods for the Cytochemical/Histochemical Localization of Plant Cell/Tissue Chemicals. Methods in Light Microscope Radioautography. Some Fluorescence Microscopical Methods for Use with Algal, Fungal, and Plant Cells. Fluorescence Microscopy of Aniline Blue Stained Pistils. A Short Introduction to Immunocytochemistry and a Protocol for Immunovi-sualization of Proteins with Alkaline Phosphatase. The Fixation of Chemical Forms on Nitrocellulose Membranes. Dark-Field Microscopy and Its Application to Pollen Tube Culture. Computer-Assisted Microphotometry. Isolation and Characterization of... 

The reaction was monitored by TLC (eluent petroleum ether-diethyl ether, 1 1). The visualization of the cinnamyl alcohol (UV active) with p-anisalde-hyde dip gave a blue stain, Rf 0.35, and a brown stain for the epoxycinnamyl alcohol, Rf 0.25. 

As the buffer used in these studies [86, 158] is hypotonic to rat peritoneal mast cells, incubations of greater than 10 min result in significant cell swelling, a large increase in membrane permeability, as judged by trypan blue staining, and eventually cell lysis. This raises some concern as to the location of the site... 

Densitometric scans of Coomassie Blue stained electrophore-tograms were obtained at 550nm, using an ISC0 gel scanner accessory in combination with an ISC0 Type 6 optical unit and Type UA-5 absorbance monitor. 

Size-based analysis by CE provides similar information and comparable limits of detection to analysis by SDS-PAGE with Coomassie blue staining.120 129 The performance of both electrophoretic techniques for the analysis of polypeptides is far superior to size exclusion chromatography. Figure 9.7 shows the separation of SDS-complexed recombinant protein standards by CE. 

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