Staining methods techniques

Farbewanne, Farbekasten staining method/technique Farbemethode, Farbetechnik stainiess steei rostfreier Stahl stamping Pragen stand/rack Slander standard Standard, Normalwert ... 

A dual isotope labeling technique [85] has been used to measure membrane permeability in plant cells, based on the selective permeabiHty of the membranes of living cells to tritiated water and carbon-14 labeled mannitol. Kieran [29] showed that the results of the dual isotope labeling and Evan s Blue staining methods correlated well as indicators of cell viability however, the latter was preferable in terms of reagent cost and ease of analysis. 

In 1958 Taylor demonstrated SCEs, using autoradiographic techniques to detect the disposition or labeled DNA following incorporation of [3H]-thymidine. 5-Bromo-2 -deoxyuridine (drdU) has now replaced [3H]-thymidine and various staining methods have been used to show the differential incorporation of BrdU between sister chromatids fluorescent Hoechst 33258 (Latt, 1973) combined fluorescent... 

Precise quantifications are an important quality in molecular biology. There are slight differences in the methods used for global and targeted proteomics. In experiments intended to visualize as many proteins as possible, it is highly desirable to have a parallel quantification method that builds on the display technique. For 2D gel electrophoresis, fluorescent staining methods are under development (Urwin and Jackson, 1993), but they still lack overall sensitivity. Labeling proteins with radioactive isotopes is the most precise method for quantification but is limited to cell cultures, and alternatives are desirable. Recently, a precise method... 

Wise and Winkelmann (10, 11) demonstrated that micro-CT can be used to evaluate rat and rabbit fetuses from developmental toxicity studies. To show compatibility with traditional staining methods, fetuses were first evaluated in a micro-CT scan and then sectioned and stained for comparison of the two methodologies. As expected when comparing discrete techniques, minor differences in the results were observed, but it was concluded overall that micro-CT imaging can effectively be used to assess rat and rabbit fetal skeletal structures. 

The pivotal reagent common to all immunohistochemical techniques is the antibody. The availability of new antisera, their immunoglobulin fractions and monoclonal antibodies to an ever-increasing number of clinically useful tissue antigens has enormously expanded the quantity and quality of the immunohistologic repertoire. To better comprehend the potential of immunohistochemical staining methods as well as associated problems, it is necessary to have a basic knowledge of antibodies, their potentials and their limitations. 

The reagents available for IHC, have increased in quality, but even more so in number of sources and variety of staining methods. This plentitude of reagents contributes to lack of standardization in significant ways, that in theory are manageable by good technique and use of proper controls, but in practice have led to requirements of such excellence in technical process, that many laboratories cannot find sufficient, or sufficiently skilled, staff to comply. 

Fetal examinations Each live fetus weighed and examined for external abnormalities Live fetuses killed and a midcoronal slice made in the head of each fetus to evaluate the contents of the cranium Internal organs of the thoracic and abdominal cavities of all fetuses examined in the fresh state using Staples s technique, and the sex of each fetus determined Viscera removed and discarded Carcasses processed for skeletal examination using the Alizarin Red S staining method and evaluated Findings judged to be variations or malformations... 

Tests currently available to identify and quantify microbial damage are reviewed by Allen et al. [484]. These tests include GUAM fluidity tests, pH determinations, reducing sugar determinations, microscopic techniques, and staining methods. 

Although the majority of laboratories use a commercially availabie method for L/S determination (Fetal-Tek, Helena Laboratories, Beaumont, Tex.), some continue to use methods developed in their own laboratories (the so-called "home brew methods). Because there are many techniques, laboratories should be very careful when establishing medical decision limits. Some methods measure all phosphatidylcholine species, both saturated and unsaturated. Different staining methods will differentially stain phos-phatidylchohne compared with the biological internal stan-... 

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