Immuno-gold

IGSS Immuno-gold silver stain IHC Immunohistochemistry IHES Idiopathic hypereosinophilic syndrome... 

Fig. 17.2. (Opposite) Immuno-gold localization of a T. muris-derived IFN-y homologue to the bacillary band and cuticular pore. (A) Transmission electron micrograph of bacillary band showing the pore chamber (PC), pore aperture (PA) and lamellar apparatus (LA) (x22,000). (B) High-power localization of antibody staining (black dots) to the lamellar apparatus (LA) and pore chamber (PC) (x36,000). (Courtesy ofF. Bughdadi.)...

De Mey, J., Moeremans, M., Ceuens, G., Nuydens, R., and De Brabander, M. (1981) High resolution light and electron microscopic localization of tubulin with the IgS (immuno gold staining) method. Cell Biol. Int. Rep. 5, 889. 

Verkleij AJ, Leunissen JLM. Immuno-Gold Labeling in Cell Biology, CRC Press, Boca Raton, FL, 1989. 

Goode, D. and Mangel, T. K. (1987) Backscattered electron imaging of immuno-gold labeled and silver-enhanced microtubules in cultured mammalian cells. 

Horisberger, M. (1989) Quantitative aspects of labeling colloidal gold with proteins, in Immuno-gold Labeling in Cell Biology (Verkleij, A. J. and Leunissen, J. L. M., eds.), CRC, Boca Raton, FL, pp. 49-60. 

Hughes, D. A, Kempson, M. G., Carter, N. P., and Morris, P. J (1988) Immuno-gold-silver/Romanowsky staining simultaneous immunocytochemical and morphological analysis of fine-needle aspirate biopsies. Transplant. Proc 20, 575,576... 

Baude A, Nusser Z, Roberts JD et al (1993) The metabotropic glutamate receptor (mGluRl alpha) is concentrated at perisynaptic membrane of neuronal subpopulations as detected by immuno-gold reaction. Neuron 11 771-87... 

Graber MB, Kreutzberg GW (1985) Immuno gold staining (IGS) for electron microscopical demonstration of glial fibrillary acidic (GFA) protein in LR white embedded tissue. Histochemistry. 83, 497-500. 

Boon ME, Kok LP, Moorlag HE, et al. Accelerated immuno-gold-silver and immunoperoxidase staining of paraffin sections with the use of microwave irradiation Eactors influencing results. Am J Clin Pathol. 1989 91 137. 

Fig. 6. The analytical device for lateral-flow immuno-gold assay. C control line, T test line.

Fig. 2. Ultrastructure of a Golgi-to-plasma-membrane carrier (GPC) formation site. A. A VSVG-GFP-transfected Cos cell was fixed at the moment of formation of a GPC (arrow), labeled with an antibody against VSVG using the immuno-gold protocol, and processed for...

These test strips were employed for the detection of human papilloma virus (amongst others) [32]. Haptenized DNA molecules (PCR amplicons from DNA samples of cervix carcinomas) were immuno-labeled with UCNPs. Subsequently, an LF assay was performed that resulted in a 100-fold improved sensitivity compared to established assays using gold nanoparticles. Improved sensitivity for amplified nucleic acid targets compared to immuno-gold and Cy5 detection-systems was also found in tests for Vibrio cholerae where attomole quantities of DNA could be detected [37]. 

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