Rapid staining method

Crissman HA, Oka MS, Steinkamp JA. Rapid staining methods for analysis of deoxyribonucleic acid and protein in mammalian cells. J Histochem Cytochem 1976 24 64-71. 

Saha DC et al., A rapid staining method for detection of endophytic fungi in turf and forage grasses. Phytopathology 78 237—239, 1988. 

Duan, L., Wang, Y., Li, S.S-C., Wan, Z., and Zhai, J. (2005) Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano-gold immunological amplification and silver staining method. BMC Infect. Dis. 5, 53. http //www.biomedcentral. com/1471-2334/5/53. 

Another example is the application of the Jones-Marres silver method for rapid staining of fungi in the brain tissue of immunocompromised patients (Boon et al., 1998). This procedure can be carried out using the MicroMED BASIC microwave lab station (Milestone, s.r.l., 24010 Sorisole, Italy). The lab station has software for reliable control of power, time, and temperature using infrared temperature control for no-touch temperature determination. It also has a 360-degree rotation carousel (no hot spots) and produces printouts of the temperature and power levels used during various microwave steps. 

The method for the rapid quantification of PHA was achieved not only by introducing the fluorescence Nile red or NBA stains as previously discussed, but by also using infrared spectroscopy. Similar to the dye staining method, infrared spectroscopy avoids the use of solvents, and requires minimal sample volume and preparation... 

The initial steps of solubilization, DEAE chromatography and gel filtration were slight modifications of reported procedures [19]. The pooled fractions from a Sephacryl S-200 (Pharmacia) column were applied to a Mono Q HR5/5 FPLC column (Pharmacia) and eluted in a 20 ml gradient from 0-350 mM NaCl in 0.25 M sucrose-20 mM Tris-HCl pH 7.3. Fractions of 1 ml were collected and assayed for binding of NAA-1-[ C] (61 mCi/mmol, Amersham) by one of three methods [20]. The most active fractions were pooled, desalted and lyophilized. This preparation (approx. 50% receptor) was used either for monoclonal antibody production or was fully purified by native PAGE in a neutral pH discontinuous system [3]. The gel was briefly electroblotted (5 min, 10 mA) to nitrocellulose and the small fraction of transferred proteins visualized by rapid staining [8]. This blot was then used to locate precisely the bulk protein bands remaining in the gel. 

Chieco, P Pagnoni, M. Romagnoli, E. Melchiorri, C. A rapid and simple staining method, using toluidine blue, for analysing mitotic figures in tissue sections. Histochem. J. 1993, 25, 569-577. 

Deka, C. Gordon, K. M. Gupta, R. Horton, A. Methods and compositions for rapid staining of nucleic acids in whole cells. U.S. Patent 6271035, 2001 Chem. Abstr. 2001, 135, 149591. 

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