Staining methods image analysis

Masuoka, S. Yoneda, K. Oka, M. Apparatus and method for analyzing spinal fluid by staining and imaging analysis. Jpn. Kokai Tokkyo Koho JP 2004132787, 2004 Chem. Abstr. 2004, 140, 371466. 

A related approach was used by Ranefall et al. (1998) to quantify images of immunohistochemically stained cell nuclear Ki-67 antigen and cyclin A protein in bladder carcinoma tissue. They combined automatic, computerized image analysis with appropriate controls and reference material. This approach is superior to the automatic method without... 

To circumvent the above limitations, the morphometric method can be used (Riischoff et al., 1990). It consists of automatic or semiquantitative measurement of the area occupied by the silver-stained structures within the nuclear profile with computer-assisted image analysis (Trerb et al., 1995). This method is faster, more accurate and reproducible, and shows less interobserver variation. Moreover, in contrast to the counting technique, the morphometric method is predictive of patient survival, independent of the clinical stage of the disease (Ofner et al., 1995a,b). 

Note that although immunohistochemistry is contributing significantly to clinical information, in the absence of quantitation, this method is subjective and prone to intra- and interobserver variations. To assure the reproducibility of histopathological results and then-correct evaluation, at least an assessment of the percentage of positive cells and both the staining pattern and intensity must be made. If available, computer-assisted image analysis... 

A number of methods are available. The author routinely used a computer assisted image analysis system. The cells which had migrated were visualized by staining the nuclei with Field s B solution. The reddish blue spots are then digitized and quantified. 

---