Antibodies against gangliosides

Not only biochemical analysis, but immunohistochemical staining with antibodies against gangliosides has similarly revealed the existence and distribution of gangliosides in epidermis. Nakakuma et al. showed that epidermal keratinocytes reacted with an anti-GM3 monoclonal antibody, but not an anti-GD3 mAb.11 Expression of GM3, predominantly in the stratum corneum, was reported by Paller et al.12 In contrast, Hersey et al. detected GD2 in the basal and spinous layers of the epidermis, whereas neither GM3 nor GD3 was detected in normal skin.13 However, the epidermis adjacent to naevi and primary melanoma strongly expressed GD3.13... 

Neisser, A., Bemheimer, H., Berger, T., Moran, A.P., Schwerer, B. Serum antibodies against gangliosides and Campylobacter jejuni lipopolysaccharides in Miller Fisher syndrome. Infect Immun 65 (1997) 4038 1042. 

Mizutani K, Oka N, Kusunoki S, Kaji R, Kanda M, Akiguchi I, Shibasaki H (2003) Amyotrophic lateral sclerosis with IgM antibody against gangliosides GM2 and GD2. Intern Med 42 277-280... 

We also investigated the specificities of purified antibodies against G i and Gp, from the serum of a rabbit immunized with bovine brain gangliosides. As shown in Figure 6, the whole antiserum had substantial activity both against the two ganglio-... 

Figure 6. Reactivities of whole antiserum and purified anti-Gll and anti-Gn,b antibodies against liposomes containing glycolipids. Antibodies purified from rabbit anti-bovine brain ganglioside serum as described previously (12). Glucose release measured from liposomes containing DMPC, CHOL, DCP, and either Cm, Gpn CMH, CDH, DDG (each 150 p.g/ymol PC) or CTH (150 nmol/pmol PC).

Ilia, I., Ortiz, N., Gallard, E., Juarez, C., Grau, J.M., Dalakas, M.C. Acute axonal Guillain-Barre syndrome with IgG antibody against motor axons following parenteral gangliosides. Ann Neurol 38 (1995) 218-224. 

Figure 5. Natural antibodies in normal human and rabbit sera against liposomes containing gangliosides GM1 or Gntb. Glucose release measured from liposomes containing DMPC, CHOL, DCP, and GM, (150 /lg/fimol PC) or G,)tb (150 fig/p/riol PC). Human serum was complement source with the GM, liposomes due to high level of anti-Gui activity in guinea pig serum (1).

The observation that GBS-associated C. jejuni isolates exhibit ganglioside mimicry prompted a number of studies that showed the reactivity of serum antibodies in GBS patients, against not only C. jejuni LOS (Yuki et al., 1993 Schwerer et al., 1995 Neisser et al., 1997, 2000), but particularly against the core OS mimicking ganglio-sides (Prendergast et al., 1999). Cross-reactive anti-GMi and -GDia antibodies were... 

Goodyear, C.S., O Hanlon, G.M., Plomp, J.J., Wagner, E.R., Morrison, I., Veitch, J., Cochrane, L., Bullens, R.W., Molenaar, P.C., Conner, J., Willison, H.J. Monoclonal antibodies raised against Guillain-Barre syndrome-associated Campylobacter jejuni lipopolysaccharides react with neuronal gangliosides and paralyze muscle-nerve preparations. J Clin Invest 104 (1999) 697-708. 

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