Immunoblot analysis

Figure 1. SDS-PAGE and Immunoblot Analysis of Purified PGl and Separated PG2 and Subunit Proteins.

Fig. 10.5. Immunoblotting analysis of the antibody response of jirds against various stages of A viteae. (A) Reaction with sera of jirds vaccinated with irradiated L3 (B) reaction of sera of A. w feae-infected jirds (C) reaction with sera of naive jirds. 1, Male antigens 2, female antigens 3, mf antigens 4, L3 antigens. Note that the reaction of vaccinated jird sera is predominantly directed against chitinase bands of 205 kDa and 67 kDa (arrows) as well as against a 17 kDa protein.

Immunoblot analysis is conducted with antibodies to the His8-tag epitope (to detect the TIF32-His8 subunit Santa Cruz) or with antibodies to the other factors. 

Cornelius RM, Shankar SP, Brash JL, Babensee JE (2011) Immunoblot analysis of proteins associated with self-assembled monolayer surfaces of defined chemistries. J Biomed Mater Res 98A 7-18... 

Fig. 4. Localization of WAP27 and WAP20 in the crude microsome fractions and the relation with marker-enzyme activities in three organelles (ER, tonoplast, and Golgi). SDS-PAGE of fractionated proteins by isopycnic linear sucrose density gradient centrifugation of microsome fraction of mulberry cortical parenchyma cells was performed using 6-pL samples in each fraction. Immunoblot analysis was performed with anti-WAP27 and anti-WAP20 antibodies. (From ref. [1], with permission from the American Society of Plant Physiologists.)...

Fic. 1. Demonstration of six Lp(a) phenotypes in individual plasma samples by SDS-PAGE and immunoblot analysis. Apo(a) phenotypes are indicated in each lane. [With permission of Sandholzer et at. (S3).]... 

The site of assembly of the Lp(a) particle, by covalent linkage of apo-B100 to apo(a), is not definitively established. White et al. (W12) proved in baboon hepatocytes that inside the cell two types of apo(a) existed, of which only the larger form was recovered from the culture medium. The lower-molecular-weight form proved to be a precursor with a prolonged residence time in the endoplasmatic reticulum. Density gradient ultracentrifugation and immunoblot analysis showed that the majority of apo(a) was secreted into the medium in a... 

Figure 8. Increased chromosome instability in ubrlA S. cerevisiae (3). (a) Sectoring assay for the chromosome loss using the SUPJJ-marked YPH277 (UBRl) strain and its ubrlA derivative (see Methods), (b) Immunoblot analysis of...

The inhibition of SuSy by the divalent cations (fCi 15 aM), Zn (fC 25 aM), and Ni (fti 37 aM) was exploited for further purification of SuSyl by immobilized metal affinity chromatography (IMAC). A subsequent gel filtration yielded homogeneous SuSyl suitable for crystallization experiments [24]. The protein chemical characterization revealed a homotetrameric organization of the 93 kDa subunit. Our kinetic data for the cleavage reaction and preliminary immunoblot analysis for phosphoserine suggested that SuSyl may be phosphorylated in the yeast expression system [28]. 

Chen YT, He JK, Ding JH, Brown BI (1987) Glycogen debranching enzyme purification, antibody characterization, and immunoblot analysis of type III glycogen storage disease. Am J Hum Genet 41 1002-1015... 

Talbot, P.V., Knobler, R.L., and Buchmeier, M. 1984. Western and dot immunoblotting analysis of viral antigens and antibodies Application to murine hepatitis virus. J. Immunol. Methods 73 177-188. 

Fig. 24 GST yeast protein analysis, a 60 samples were examined by immunoblot analysis using anti-GST 19 representative samples are shown, b 6566 protein samples representing 5800 unique proteins were spotted in duplicate onto a single nickel-coated microscope slide which was then probed with anti-GST. c Enlarged image of one of the 48 blocks [ 143]...

Figure 1 Immunoblot analysis of CYP3A (A) and CYP2B (B) protein in rat hepatocytes cultured on Matrigel and collagen. Cells were incubated in the presence of 10-p.M dex-amethasone or 50-p.M phenobarbital for 48 hours. Source From Ref. 12.

Both functional assays [vincristine transport (381) and rhodamine 123 transport (383)] and biochemical assays involving immunohistochemical analysis (381,384) have confirmed the expression of P-gp in the luminal membrane of BMECs cultured on polycarbonate membranes. Additionally, immunohistochemical methods showed the expression of P-gp in BMEC to be constant and at a high level in five- to seven-day-old old primary cultures (384). Like many other barrier-forming cells, BMECs appear to express other efflux proteins, for example, RT-PCR and immunoblot analysis have shown the presence of MRP1 in rat BMECs (385,386). Functional evidence has also been presented to confirm the expression of MRP1 in BMECs (387). 

The human osteosarcoma cell line has been shown selectively to express COX-2 by reverse transcription-polymerase chain reaction and immunoblot analysis, whereas undifferentiated human lymphoma U937 cells... 

Hamilton, R. G., Reimer, C. B., and Rodkey, L. S. (1987). Quality control of murine monoclonal antibodies using isoelectric focusing affinity immunoblot analysis. Hybridoma 6, 205-217. 

CoUer BS, Seligsohn U, Little PA T pe I Glananatm thrombasthenia patients from the Iraqi-Jewish and Arab populations in Israel can be differentiated by platdet glycoprotein Ilia immunoblot analysis. Blood 69 1969-1703,1987. 

Sdigsohn U, CoUer BS, Zivelin A, Plow EF, Ginsberg MH Immunoblot analysis of platelet glycoprotein lib in patients with Glanzmann thrombasthenia in Israel. Br J Haematol 72 415-423,1989. 

Alternatively the cells are metabalically labeled with 50(j.Ci [2- H]-adenine per ml of medium for 16 h (Staddon etal., 1991). Labeled actin can be identified by immunoprecipitation with anti-actin antibody (from Boehringer or ICN) or by immunoblot analysis of cell lysates electrophoretically resolved on 2D gels. 

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