Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Mass spectrometry imaging

Imaging mass spectrometry involves MS performed on tissue sections mounted on a MALDI plate. The mass spectra generate images and an in situ protein expression profile of the specimen is analyzed. Specifically, the frozen tissue sections applied to a MALDI plate are subjected to laser interrogation and analyzed at regular spatial intervals. The mass spectral data obtained at different intervals are compared to generate a spatial distribution of masses (proteins) across the tissue section. [Pg.385]

Analyses using this approach have revealed more than 1500 protein peaks from histologically selected 1 mm diameter regions of single frozen sections.52 Imaging MS allows investigators to [Pg.385]

The preparation of samples for mass spectrometric analysis almost always requires recovery of the analyte from a matrix, often followed by further preparative steps prior to introduction of the sample into the ion source. Such destructive processes are unacceptable when the objective is to determine the specific location of a particular compound within a biological sample, e.g., the location of a certain lipid in a tissue, and led to the development of imaging mass spectrometry with MALDI as the ionization method, although DESI is also applicable. [Pg.194]

Imaging is usually carried out on frozen tissue sections ( 20 microns thick) placed on a MALDI plate. The chemical nature of the compounds of interest determines [Pg.194]

The spatial resolution obtainable within tissues, that are now approaching the cellular level, is a compromise between the size of the laser spot and the sensitivity of the mass spectrometer (Section 4.13). The mass range over which MALDI can be used is limited by interferences from the matrices the lower limit is 500-600 Da. DESl is not limited at lower masses and can facilitate analysis of compounds with masses of 500 Da. [Pg.195]

Spectra taken on a transect across a tissue section vary. [Pg.195]

Some compounds are unique to certain parts of the tissue and may be diagnostic. [Pg.195]


Groseclose MR, Massion PP, Chaurand P, et al. High-throughput proteomic analysis of formalin-fixed paraffin-embedded tissue microarrays using MALDI imaging mass spectrometry. Proteomics 2008 8 3715-3724. [Pg.331]

Flowever, the object being analyzed has to be removed from the tissues. Thus, information about the distribution of the target in the organism or in the cells is inevitably lost. What is now needed is a technology to acquire information about the distribution of the biomolecule simultaneously with its identification. The method used for this purpose, called imaging mass spectrometry (IMS), is as follows. The tissue sample is cut into thin slices, and a matrix that assists the ionization of macromolecules is spread onto these slices. The macromolecules are then ionized by a scanning laser, and the generated ions are detected and analyzed by MS.1... [Pg.369]

Shimma S, Setou M. Review of imaging mass spectrometry../. Mass. Spectrom. Soc. Jpn. 2005 53 230-238. [Pg.387]

KJinkert I, McDonnell LA, Luxembourg SL, et al. Tools and strategies for visualization of large image data sets in high-resolution imaging mass spectrometry. Rev. Sci. Instrum. 2007 78 053716. [Pg.387]

Sugiura Y, Shimma S, Setou M. Two-step matrix application technique to improve ionization efficiency for matrix-assisted laser desorption/ionization in imaging mass spectrometry. Anal. Chem. 2006 78 8227-8235. [Pg.388]

Chaurand P, Schwartz S, Billheimer D, et al. Integrating histology and imaging mass spectrometry. Anal. Chem. 2004 76 1145-1155. [Pg.388]

Groseclose M, Anderson M, Hardesty W, et al. Identification of proteins directly from tissue in situ tryptic digestions coupled with imaging mass spectrometry. J. Mass. Spectrom. 2007 42 254-262. [Pg.388]

Meistermann H, Norris J, Aerni H, et al. Biomarker discovery by imaging mass spectrometry transthyretin is a biomarker for gentamicin-induced nephrotoxicity in rat. Mol. Cell. Proteomics 2006 5 1876-1886. [Pg.388]

Shimma S, Sugiura Y, Hayasaka T, et al. MALDI-based imaging mass spectrometry revealed abnormal distribution of phospholipids in colon cancer liver metastasis. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2007 855 98-103. [Pg.388]

Yao I, Sugiura Y, Matsumoto M, et al. In situ proteomics with imaging mass spectrometry and principal component analysis in the Scrapper-knockout mouse brain. Proteomics 2008 8 3692-3701. [Pg.388]

Altelaar A, Luxembourg S, McDonnell L, et al. Imaging mass spectrometry at cellular length scales. Nat. Protoc. 2007 2 1185-1196. [Pg.389]

In a separate study, a protocol for Matrix-assisted laser desorption-ionization (MALDI) imaging mass spectrometry (IMS) has been proposed.18 This IMS technique provides a new approach to visualize spatial distribution of thousands of molecular species, including peptides, proteins, and their metabolites in two- or three-dimensional levels. This approach may also provide a straightforward method of determining the tissue distribution of multiple peptides or proteins in a quantitative manner.18 Chu et al.19 reported a nondestructive molecular extraction method to obtain proteins from a single FFPE or frozen tissue section, without destroying the tissue morphology, such... [Pg.394]

Andersson M, Groseclose MR, Deutch AY, et al. Imaging mass spectrometry of proteins and peptides 3D volume reconstruction. Nat. Methods 2008 5 101-108. [Pg.397]

M. Stoeckli, P. Chaurand, D. E. Hallahan and R. M. Caprioli, Imaging mass spectrometry A new technology for the analysis of protein expression in mammalian tissues, Nature Med., 7, 493 496 (2001). [Pg.73]

D. S. Cornett, M. L. Reyzer, P. Chaurand, and R. M. Caprioli. MALDI Imaging Mass Spectrometry Molecular Snapshots of Biochemical Systems. Nat. Meth., 4(2007) 828-833. [Pg.81]

In the literature these studies are classified as imaging mass spectrometry (IMS) and defined as the investigation of the chemical profile of a sample surface with a submicron lateral resolution and chemical specificity. The main aim is to use the power of mass spectrometry techniques to create chemical images showing the distribution of compounds ranging in size from atomic ions and small molecules to large proteins. [Pg.275]


See other pages where Mass spectrometry imaging is mentioned: [Pg.336]    [Pg.392]    [Pg.45]    [Pg.385]    [Pg.77]   
See also in sourсe #XX -- [ Pg.385 ]

See also in sourсe #XX -- [ Pg.275 , Pg.276 ]

See also in sourсe #XX -- [ Pg.360 ]

See also in sourсe #XX -- [ Pg.113 , Pg.399 , Pg.452 ]

See also in sourсe #XX -- [ Pg.246 , Pg.247 , Pg.248 ]

See also in sourсe #XX -- [ Pg.333 , Pg.355 ]

See also in sourсe #XX -- [ Pg.417 , Pg.426 , Pg.430 , Pg.435 ]

See also in sourсe #XX -- [ Pg.159 , Pg.194 , Pg.252 , Pg.261 ]

See also in sourсe #XX -- [ Pg.507 , Pg.508 , Pg.509 ]

See also in sourсe #XX -- [ Pg.3 , Pg.4 , Pg.5 , Pg.6 , Pg.7 , Pg.8 , Pg.9 , Pg.10 , Pg.11 , Pg.12 , Pg.13 , Pg.14 , Pg.70 , Pg.78 , Pg.115 , Pg.132 , Pg.137 , Pg.143 , Pg.147 , Pg.148 , Pg.149 , Pg.150 , Pg.151 , Pg.152 , Pg.153 , Pg.154 , Pg.155 , Pg.156 , Pg.157 , Pg.159 , Pg.160 , Pg.161 , Pg.162 , Pg.163 , Pg.164 , Pg.165 , Pg.166 , Pg.167 , Pg.168 , Pg.169 , Pg.170 , Pg.173 , Pg.174 , Pg.175 , Pg.176 , Pg.177 , Pg.178 , Pg.179 , Pg.180 , Pg.181 ]

See also in sourсe #XX -- [ Pg.259 , Pg.260 , Pg.261 , Pg.262 , Pg.263 , Pg.264 , Pg.265 , Pg.266 , Pg.267 , Pg.268 , Pg.269 , Pg.270 , Pg.271 , Pg.272 ]

See also in sourсe #XX -- [ Pg.246 , Pg.247 , Pg.250 , Pg.251 , Pg.254 , Pg.256 , Pg.257 , Pg.258 ]




SEARCH



And imaging mass spectrometry

Imaging Mass Spectrometry of Lipids

Imaging Mass Spectrometry of Medical Tissues

Imaging by Inorganic Mass Spectrometry in Materials Science

Imaging by Mass Spectrometry

Imaging ion mobility-mass spectrometry

Imaging mass spectrometry MALDI matrix application

Imaging mass spectrometry MALDI, principles

Imaging mass spectrometry advantage

Imaging mass spectrometry biological tissues

Imaging mass spectrometry defined

Imaging mass spectrometry fundamentals

Imaging mass spectrometry matrix application methods

Imaging mass spectrometry matrix selection

Imaging mass spectrometry matrix-assisted laser desorption/ionization

Imaging mass spectrometry membrane

Imaging mass spectrometry production

Imaging mass spectrometry protein mapping, tissue section

Imaging mass spectrometry sample preparation

Imaging mass spectrometry small molecules

Imaging mass spectrometry studies

Imaging mass spectrometry with MALDI

Imaging mass spectrometry with SIMS

Imaging time of flight secondary Ion mass spectrometry

Lipid distribution, imaging mass spectrometry

MALDI mass spectrometry imaging

MALDI-ToF imaging mass spectrometry

Mass spectrometry imaging applications

Mass spectrometry imaging data processing

Mass spectrometry imaging database, proteins

Mass spectrometry imaging developments

Mass spectrometry imaging ionization beam

Mass spectrometry imaging ionization techniques (

Mass spectrometry imaging lipids

Mass spectrometry imaging matrix application

Mass spectrometry imaging metabolites

Mass spectrometry imaging properties

Mass spectrometry imaging strategies

Mass spectrometry imaging surface, tissue sections

Mass spectrometry imaging technological developments

Mass spectrometry imaging tissue treatment

Matrix-assisted laser desorption imaging mass spectrometry

Matrix-assisted laser desorption/ionization in imaging mass spectrometry

Proteomics analysis, imaging mass spectrometry

Raster Mass Spectrometry Imaging

Secondary ion mass spectrometry imaging

Tissue Mass Spectrometry Imaging

© 2024 chempedia.info