Imaging mass spectrometry membrane

Grey A, Chaurand P, Caprioli R, Schey K (2009) MALDI imaging mass spectrometry of integral membrane protein from ocular lens and retinal tissue. J Proteome Res 8 3278-3283. 

Sjovall, P., Lausmaa, J., Nygren, H., Carlsson, L., and Malmberg, P. (2003). Imaging of membrane lipids in single cells by imprint-imaging time-of-flight secondary ion mass spectrometry. Anal. Chem. 75 3429-3434. 

Figure 6. Mass spectrometry imaging of a capture membrane processed with molecular scanner on a mouse brain (CPu = caudate putamen (striatum) Ml = primary motor cortex VIM = primary visual cortex, monocular region).

Although information on the spatial distribution of membrane lipids can be important (3), most mass-spectrometric approaches used in lipidomics focus currently on the determination of total lipid composition. An advanced mass-spectrometric surface analysis technique, however, secondary ion mass spectrometry (SIMS) determines lipid compositions within different areas of freeze-fractured cells (12). This technique and related imaging techniques will become important tools in lipidomics. 

Pacholski, M.L. et al., Static time-of-flight secondary ion mass spectrometry imaging of freeze-fractured, frozen-hydrated biological membranes, Rapid Commun. Mass Spectrom., 12, 1232, 1998. 

Mass spectrometry imaging with time-of-flight secondary ion mass spectrometry (ToF-SIMS) has revealed the spatial distribution of chemicals on a surface (1). When applied to biological samples, this method offers spatial information on biologically relevant small molecules (<1,000 Da). Indeed, ToF-SIMS imaging has been shown to be a powerful analytical tool for mapping the distribution of lipids on a cell membrane at the single-cell... 

Cannon, D. M. J., Pacholski, M. L., Winograd, N., Ewing, A. G. (2000) Molecule specific imaging of freeze-fractured, frozen hydrated model membrane systems using mass spectrometry. JAm Chem Soc, 122, 603-610. 

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