Mouse knockout

Insight from the PPARa Knockout Mouse. PPARa-deficient adult mice are viable, fertile, and healthy, indicating that PPARa is not essential for embryonic development. When adult PPARa-7- mice are treated with fibrates, the characteristic response to PP is abolished, with no liver weight increase, no increase in... 

A key requirement of QSAR is that the compounds used in the modeling and prediction processes should have the same mechanism of action, and for this reason most QSAR studies are made with congeneric series of compounds. However, if a diverse set of compounds can reasonably be assumed to have the same mechanism of action, QSAR modeling can justihably be carried out. For example, Dearden et al. [43] developed a QSAR for the ratio of brain levels of 22 very diverse drugs in the wild-type mouse and the P-glycoprotein knockout mouse (R+/ ) ... 

H3 knockout mouse H3 KO mouse is heavier than wild type mice [61] but see also [62]... 

Rosenfeld ME, Polinsky P, Virmani R, Kauser K, Rubanyi G, Schwartz SM. Advanced atherosclerotic lesions in the innominate artery of the Apo E knockout mouse. Arterioscler Thromb Vase Biol 2000 20(12) 2587-2592. 

One thing that is interesting about the p27 knockout mouse is that cell death does not bring cell numbers down to normal levels. Why not It may be because there are more cells in multiple lineages in each organ, and they support the survival of one another. If only one cell type is increased within an organ, then cell death would presumably bring the number back down to normal. 

Hu, W, Jiang, A, Liang, J, Meng, H, Chang, B, Gao, H, and Qiao, X, 2008. Expression of VLDLR in the retina and evolution of subretinal neovascularization in the knockout mouse model s retinal angiomatous proliferation. Invest Ophthalmol Vis Sci 49, 407 115. 

In finalizing this series of studies, the double receptor knockout mouse (OX,k / ()X2k ) was shown to be phenotypically indistinguishable from orexin mice (Kisanuki et al, 2001), a result that makes the existence of additional orexin receptors unlikely. 

Although many animal models for iron overload exist, some mimicking certain aspects of HH, the 32-microglobulin knockout mouse is of special interest as it revealed for the first time crucial aspects of the pathogenesis of human HH in an animal model, and also because it underlines the important links between iron metabolism and the immune system. Hepatic iron overload in 32-microglobulin ( 32m)-deficient mice appeared to be similar to that found in HH, with pathological iron depositions occurring predominantly in liver parenchymal cells (de Sousa et ah,... 

The first gene-knockout mouse to become available was a mouse lacking detectable P-gp in the brain capillary endothelial cells. This has been used to elegantly... 

Yao I, Sugiura Y, Matsumoto M, et al. In situ proteomics with imaging mass spectrometry and principal component analysis in the Scrapper-knockout mouse brain. Proteomics 2008 8 3692-3701. 

Nelson We have the phospholamban knockout mouse model, and John Lederer was able to peel out a component of the decay that was due to reuptake. We didn t see any difference between the control and the knockouts. Presumably it is happening, but we couldn t see any difference in the decay in the phospholamban knockouts, as was seen in heart muscle, nor could we see any effect of lanthanum or zero Na+. Examining the decay of the spark would be a good indicator of local Ca2+ removal, though. It would also be worth examining the decay of the BK current. 

McCarron In a knockout mouse lacking the type 1 InsP3 receptor, the excitatory junction potentials are reduced in gastrointestinal smooth muscle. 

Nelson It could. We could try to load the SR. For example, we have used the phospholamban knockout mouse where it appears that the SR Ca2+ load increases (Wellman et al 2001). The spark frequency increases as the load increases. At the moment we have not observed any communication from the SR to the SK channels. 

Gene-targeted (knockout) mouse models provide one avenue for assessing the in vivo functional significance of particular proteins. A complementary approach is the expression of the protein of interest using transgenic methodology. There... 

Doran, A., Obach, R. S., Smith, B. J., et al. (2005) The impact of P-glycoprotein on the disposition of drugs targeted for indications of the central nervous system evaluation using the MDRIA/IB knockout mouse model. Drug Metab. Dispos. 33, 165-174. 

Dap 12 and FcRy-chain genes DAP12 and FcRy-chain More severe osteopetrosis with earlier development than in DAP 12 knockout mouse, tooth eruption Not characterized... 

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