Tissue Mass Spectrometry Imaging

Vidova, V. Novak, R Strohalm, M. Pol, J. Havlicek, V. Volny, M. Laser Desorption-Ionization of Lipid Transfers Tissue Mass Spectrometry Imaging Without MALDI Matrix. Anal. Chem. 2010,82,4994-4997. 

Djidja M, Claude E, Snel M, Francese S, Scriven P, Cardan V, Clench M (2010) Novel molecular tumor classification using MALDI-mass spectrometry imaging of tissue micro-assay. Anal Bioanal Chem 397 587-601. doi 10.1007/s00216-3554-6... 

Wiseman J, Ifa D, Zhu Y, Kissinger C, Manicke N, Kissinger P, Cooks R (2008) Desorption electrospray ionization mass spectrometry imaging drug and metabolites in tissues. Proc Natl Acad Sci USA 105(47) 18120-18125. doi 10.1073/pnas.0801066105... 

Trim P, Henson C, Avery J, McEwen A, Snel M, Claude E, Marshall P, West A, Princivalle A, Clench M (2008) Matrix-assisted laser desorption/ionization-ion mobility separation-mass spectrometry imaging of vinblastine in whole body tissue sections. Anal Chem 80 8628-8634. doi 10.1021/ac8015467... 

Agar N, Yang H, Carroll R, Lack P, Agar J (2007) Matrix solution fixation histology-compatible tissue preparation for MALDI mass spectrometry imaging. Anal Chem 79(19) 7416-7423. doi 10.1021/ac071460e... 

Mass spectrometry imaging (MSI) circumvents these issues by combining MS with microscopy (5). Spatially resolved mass spectrometric experiments directly on the surface of tissue sections enable the reconstruction of images and therefore the localization of hundreds of molecules within their histological context without any labeling. MSI has therefore received considerable interest as an imaging technique for the ex vivo molecular interrogation of animal, human, or plant tissue sections (6). 

L.A. Klerk, P.Y. Dankers, E.R. Pt ta, A.W. Bosman, M.E. Sanders, K.A. Reedquist, R.M. Heeren, TOF-secondary ion mass spectrometry imaging of polymer scaffolds with surrounding tissue after in vivo implantation. Anal. Chem. 82 (2010) 4337-4343. 

Djidja, M-C. Sutton, C.W. Loadman, P.M. Scriven, R Snel, M.R Claude, E. Clench, M.R. Visualisation and in situ characterisation of proteins in adenocarcinoma tissue sections by direct MALDl-mass spectrometry imaging. Proc. 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO, June 1-5, 2008, WP 169. 

Eberlin, L.S., Liu, X., Ferreira, C.R., Santagata, S., Agar, N.Y., and Cooks, R.G. 2011, Desorption electrospray ionization then MALDI mass spectrometry imaging of lipid and protein distributions in single tissue sections, Anal. Chem., 15 8366-8371. 

Ronci M, Rudd D, Guinan T, Benkendorff K, Voelcker NH (2012) Mass spectrometry imaging on porous silicon investigating the distribution of bioactives in marine mollusc tissues. Anal Chem 84 8996-9001... 

Key words Mass spectrometry, imaging, ambient, direct analysis, depth profiling, three-dimensional, in vivo, tissue imaging. 

Key words Mass spectrometry imaging, desorption electrospray ionization, whole body, tissue... 

Key words Matrix-assisted laser desorption/ionization, time-of-fight, mass spectrometry, mass spectrometry imaging, formalin fixed and paraffin embedded, frozen tissue, on tissue digestion, proteins identification. 

It is clear that mass spectrometry imaging has great potential as a comprehensive analysis technique for endogenous peptides, particularly with a hardware configuration as evaluated in this chapter, where MALDI produced ions are analyzed in an ion trap - Orbitrap hybrid instrumentation. A single experiment can provide high mass accuracy data in combination with the spatial distribution of peptides in the tissue sample. With MS/MS experiments the molecular identity (accurate mass measurements complemented with MS" sequence data) can be confirmed firom a single or few scans only and from a few laser shots in total. 

For mass spectrometry imaging, it is important to prevent the tissue from completely drying as this will cause bead clumping and may reduce incorporation of analyte into the MALDI matrix crystals. Excess sample drying is prevented by immediately applying MALDI matrix after stretching while the tissue is still partially wet. Thus, if more than one... 

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