Imaging Mass Spectrometry of Lipids

Almost all types of ionization sources used for MS have been successfully applied for MS of lipids such as MALDI [11], DESI [12], SMS [13], and pressurized liquid extraction surface analysis [14]. In general, the majority of the lipids present in tissues ionize easily due to their polar heads (Chapters 1 and 2). PC, SM, and cholesterol species more selectively ionize in the positive-ion mode, while PI, PS, and sulfatide 

Upidomics Comprehensive Mass Spectrometry of Lipids, First Edition. Xianlin Han. 2016 John Wiley Sons, Inc. Published 2016 by John Wiley Sons, Inc. 

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Girod M, Shi Y, Cheng J, Cooks G (2010) Desorption electrospray ionization imaging mass spectrometry of lipids in rat spinal cord. J Am Soc Mass Spectrom 21 1177-1189. doi 10.1016/j. jasms.2010.03.028... 

Thomas, A., Charbonneau, J.L, Foumaise, E., and Chaurand, P. (2012) Sublimation of new matrix candidates for high spatial resolution imaging mass spectrometry of lipids enhanced information in both positive and negative polarities after l,5-diaminonapthalene deposition. Anal. Chem., 84, 2048-2054. 

Sugiura, Y., Konishi, Y., Zaima, N., Kajihara, S., Nakanishi, H., Taguchi, R. and Setou, M. (2009) Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry. J. Lipid Res. 50, 1776-1788. 

Veloso A, Astigarraga E, Barreda-Gomez G, Manuel I, Ferrer I, Giralt M, Ochoa B, Fresnedo O, Rodriguez-Puertas R, Fernandez J (2011) Anatomical distribution of lipids in human brain cortex by imaging mass spectrometry. J Am Soc Mass Spectrom 22 329-338. doi 10.1007/ s 13361-010-0024-5... 

The preparation of samples for mass spectrometric analysis almost always requires recovery of the analyte from a matrix, often followed by further preparative steps prior to introduction of the sample into the ion source. Such destructive processes are unacceptable when the objective is to determine the specific location of a particular compound within a biological sample, e.g., the location of a certain lipid in a tissue, and led to the development of imaging mass spectrometry with MALDI as the ionization method, although DESI is also applicable. 

Zaima, N., Goto-Inoue, N., Adachi, K., and Setou, M. 2011. Selective analysis of lipids by thin-layer chromatography blot matrix-assisted laser desorption/ionization imaging mass spectrometry, J. Oleo ScL, 60 93-98. 

ADVANTAGES AND DRAWBACKS OF IMAGING MASS SPECTROMETRY FOR ANALYSIS OF LIPIDS... 

Jones, E.E., Powers, T.W, Neely, B.A., Cazares, L.H., Troyer, D.A., Parker, A.S. and Drake, R.R. (2014) MALDI imaging mass spectrometry profiling of proteins and lipids in clear cell renal cell carcinoma. Proteomics 14, 924-935. 

Dill, A.L., Ifa, D.R., Manicke, N.E., Costa, A.B., Ramos-Vara, J.A., Knapp, D.W. and Cooks, R.G. (2009) Lipid profiles of canine invasive transitional cell carcinoma of the urinary bladder and adj acent normal tissue by desorption electrospray ionization imaging mass spectrometry. Anal. Chem. 81, 8758-8764. 

Sjovall, P., Lausmaa, J., Nygren, H., Carlsson, L., and Malmberg, P. (2003). Imaging of membrane lipids in single cells by imprint-imaging time-of-flight secondary ion mass spectrometry. Anal. Chem. 75 3429-3434. 

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