Mass spectrometry imaging strategies

In Chapter 4, titled Principles of Mass Spectrometry Imaging Applicable to Thin-Layer Chromatography, the authors first introduce a budding analytical approach known as imaging mass spectrometry (IMS) strategy and then present some successful examples of its practical applications. Then, they introduce in detail three mass spectrometric techniques as those routinely used within the framework of IMS. These are secondary mass spectrometry (SIMS), matrix-assisted laser desorption/ ionization (MALDI-IMS), and desorption electrospray ionization (DESI). Finally, the authors discuss the advances and bottlenecks of these techniques when applied to TLC. 

KJinkert I, McDonnell LA, Luxembourg SL, et al. Tools and strategies for visualization of large image data sets in high-resolution imaging mass spectrometry. Rev. Sci. Instrum. 2007 78 053716. 

Cheng, Z., Winant, R. C., and Gambhir, S. S. (2005). A new strategy to screen molecular imaging probe uptake in cell culture without radiolabeling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J. Nucl. Med. 46 878-886. 

Clench, M. Burrell, M. Trim, P.J. Earnshaw, C. Francese, S. Muharib, T. Anderson, D.M.G. Atkinson, S. Strategies for small molecule imaging by MALDI-MSI. Pmc. 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO, June 2008, WOG pm 2 50. 

Fig. 18.1. Strategies for direct anaiysis and imaging mass spectrometry of FFPE, PAF, and frozen tissue section.

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