Galectins

The N-terminal sequence of one peptide from the 35 kDa zone of H-gal-GP showed some homology to cathepsin B-like cysteine proteases. Molecular cloning has also identified a thrombospondin homologue associated with the diffusely staining region between zones A and B, a galectin associated with zone D (Newlands et al., 1999) and a low molecular weight (approximately 13 kDa) cysteine protease inhibitor, cystatin. 

Newlands, G.F.J., Skuce, P.J., Knox, D.P., Smith, S.K. and Smith, W.D. (1999) Cloning and characterisation of a (3-galactoside-binding protein (galectin) from the gastrointestinal nematode Haemonchus contortus. Parasitology 119, 483-490. 

K. N. Konstantinov, B. A. Robbins, and F.-T. Liu, Galectin-3, a /l-galactoside-binding animal lectin, is a marker of anaplastic large-cell lymphoma, Am. J. Path., 148 (1996) 25-30. 

S. Akahani, P. Nangia-Makker, H. Inohara, H.-R. Kim, and A. Raz, Galectin-3 A novel antiapoptotic molecule with a functional BH1 (NHGR) domain of bcl-2 family, Cancer Res., 57 (1997) 5272-5276. 

L.-G. Yu, N. Andrews, Z. Zhao, D. McKean, J. F. Williams, L. J. Connor, O. V. Gerasimenko, J. Hilkens, J. Hirabayashi, K. Kasai, and J. M. Rhodes, Galectin-3 interaction with thomsen-friedenreich disaccharide on cancer-associated MUC1 causes increased cancer cell endothelial adhesion, /. Biol. Chem., 282 (2007) 773-781. 

K. E. Pace, C. Lee, P. L. Stewart, and L. G. Baum, Restricted receptor segregation into membrane microdomains occurs on human T cells during apoptosis induced by galectin-1, J. Immunol., 163 (1999) 3801-3811. 

The deprotected lactosides were evaluated as inhibitors against lectin binding in a solid-phase inhibition assay with immobilized ASF on the surface of microtiter plate wells, mimicking cell-surface presentation, while mammalian galectins-1, -3, and -5 were in solution. Strong multivalency effects and selectivity were observed for the... 

R. M. J. Liskamp, H.-J. Gabius, and R. J. Pieters, Rigidified multivalent lactose molecules and their interactions with mammalian galectins A route to selective inhibitors, Org. Biomol. Chem., 1 (2003) 803-810. 

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