Diffusion staining

The N-terminal sequence of one peptide from the 35 kDa zone of H-gal-GP showed some homology to cathepsin B-like cysteine proteases. Molecular cloning has also identified a thrombospondin homologue associated with the diffusely staining region between zones A and B, a galectin associated with zone D (Newlands et al., 1999) and a low molecular weight (approximately 13 kDa) cysteine protease inhibitor, cystatin. 

Figure 5. Staining for Her2 protein following antigen retrieval performed at different pH. (a) Following antigen retrieval in citrate buffer pH6.0 there was only focal staining compared to (b) diffuse staining when retrieval was performed in citrate/EDTA buffer at pH8.0.

Eabeling for S-100 protein is more frequently seen in epithelioid MPNSTs than in other forms of that tumor—at least 70% of cases, often with a strong and diffuse staining pattern. The majority of these tumors... 

FIGURE 14.35 A, Esophageal melanoma. Subjacent to a normal squamous mucosa, undifferentiated, dyshesive neoplastic cells expand the submucosa. B, Esophageal melanortia. Tyrosinase diffusely stains the neoplastic cells with moderate intensity. Many cells have a perinuclear dot, Colgi-zone accentuation in addition to homogeneous cytoplasmic staining. 

The photopolymerization of the spacer gel is effected, the comb is carefully removed (in order not to destroy the surface of the wells), the plates are mounted into the electrophoretic apparatus, and samples are placed in the wells. After having filled the electrode vessels with buffer the electrophoretic separation can be started. Diffusion staining and destaining of the gel is done after disassembling the plates. 

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