Cloning, molecular

J. Sambrook, E. E. Eritsch, and T. Maniatis, Molecular Cloning. A. Eaboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989. 

The opioid peptides vary in their binding affinities for the multiple opioid receptor types. Leu- and Met-enkephalin have a higher affinity for 5-receptors than for the other opioid receptor types (68), whereas the dynorphin peptides have a higher affinity for K-sites (69). P-Endorphin binds with equal affinity to both p- and 5-receptors, but binds with lower affinity to K-sites (70). The existence of a P-endorphin-selective receptor, the S-receptor, has been postulated whether this site is actually a separate P-endorphin-selective receptor or is a subtype of a classical opioid receptor is a matter of controversy (71,72). The existence of opioid receptor subtypes in general is quite controversial although there is some evidence for subtypes of p- (73), 5-(74), and K-receptors (72,75), confirmation of which may be obtained by future molecular cloning studies. 

T. Maniatis, E. F. Fritsch, and J. Sambrook, Molecular Cloning, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1983, pp. 150—171. 

Maniatis, T, Frisch, E. R, and Sambrook, J., 1989. Molecular Cloning A Laboratory Manual, 2nd ed. Cold Spring Harbor, NY Cold Spring Harbor Laboratory. 

Berger, S. L., and Kimmel, A. R., eds., 1987. Guide to Molecular Cloning Techniques. Methods in Enzymology, Volume 152. New York Academic Press. 

Lee, D. H., et al. (1993). Molecular cloning and genomic organization of a gene for luciferin-binding protein from the dinoflagellate Gonyaulax polyedra. J. Biol. Chem. 268 8842-8850. 

Noguchi, M., Tsuji, F. I., and Sakaki, Y. (1986). Molecular cloning of apoae-quorin cDNA. Method. Enzymol. 133 298-306. 

Tatsumi, H., Kajiyama, N., and Nakano, E. (1992). Molecular cloning and expression in Escherichia coli of a cDNA clone encoding luciferase of a firefly, Luciola lateralis. Biochim. Biophys. Acta 1131 161-165. 

Clos, J., Westwood, J.T., Becker, P.B., Wilson, S., Lambert, K., Wu, C. (1990). Molecular cloning and expression of a hexameric Drosophila heat shock factor subject to negative regulation. Cell 63, 1085-1097. 

Maniatis, T. Molecular Cloning—A Laboratory Manual-, Cold Springs Harbor Laboratory Cold Springs Harbor, NY 1982, p. 324-325. 

Chang, C. Meyerowitz, E.M. (1986). Molecular cloning and DNA sequence of the Arabidopsis thaliana aleohol dehydrogenase gene. Proceedings of the National Academy of Sciences, USA, 83, 1408-12. 

Kitaura M, Nishimura M, Kakizaki M, Nomiyama H, Yoshie O Molecular cloning of a novel human CC chemokine EBIl-ligand chemokine that is a specific functional ligand for EBIl, CCR7. J Biol Chem 1997 272 13803-13809. 77... 

S Molecular cloning and characterization of a novel 62 type of histamine receptor preferentially expressed in leukocytes. J Biol Chem 2000 275 36781. 

Grunwald T, Bockisch B, Spillner E, Ring J, Brede-horst R, Ollert M Molecular cloning and expression and expression in insect cells of honey bee venom allergen acid phosphatase (Api m3). J Allergy Clin Immunol 2006 117 848-854. 

Maniatis, T Eritsch, EE, and Sambrook 1 (1982), Molecular Cloning, Cold Spring Harbor Laboratory. Martin, EH (1985), in Hopps, HE, and Petricciani, 1C, eds. Abnormal Cells New Products Risk Tissue Culture Assn., Gaithersburg, MD, pp 90-93. 

Harris RA et al Molecular cloning of the branched-chain a-ke-toacid dehydrogenase kinase and the CoA-dependent methyl-... 

A clone is a large population of identical molecules, bacteria, or cells that arise from a common ancestor. Molecular cloning allows for the production of a large number of identical DNA molecules, which can then be charac-... 

Neural progenitor cells Lawrence et al. (2004) When nestin+ multi-potential cells are differentiated towards a neuronal lineage post-infection, there is a negligible increase in virus rephcation. When these cells were transfected with a pNL4-3 molecular clone and stimulated with TNP-a, there is an upregulation in virus production, suggestive of reactivation from latency... 

Cerretti DP, Kozlosky CJ, Mosley B et al (1992) Molecular cloning of the interleukin-1 beta converting enzyme. Science 256 97-100... 

Pulciani S, Santos E, Lauver AV, et al. 1982b. Oncogenes in human tumor cell lines. Molecular cloning of a transforming gene from human bladder carcinoma cells. Proc Natl Acad Sci USA 79 2845-2849. 

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