Lectin binding

An extra-bulbar olfactory pathway (EBOP) is present in teleosts and in some non-teleost genera. Olfactory fibres run within the medial forebrain bundle, and can be traced (by SBA lectin binding) beyond the olfactory bulb into areas such as the ventral telencephalon, and/or the preoptic nucleus (Hofmann and Meyer, 1995). The projection of the EBOP fibres is similar in the sturgeon, but in other non-teleosts the primary olfactory fibres reach diencephalic target nuclei. 

Membrane extracts from adult H. contortus were enriched 24-fold for cysteine protease activity by passage over a Thiol-Sepharose affinity column and the proteins obtained (abbreviated as TSBP) were clearly localized to the microvillar surface of the intestinal cells (Knox et al., 1995,1999). TSBP comprised a prominent 60 kDa protein and several minor bands between 35 and 45 kDa and 97 to 120 kDa (Fig. 13.2). Protease activity at 38, 52 and 70 kDa was attributable to cysteine proteases and at 70 and 88 kDa to serine/metalloproteases, as judged by inhibition analyses. Lectin-binding studies showed that most of the TSBPs were glycosylated. Expression library... 

While the mechanisms of formation of noncovalent crosslinked lattices of lectins with multivalent carbohydrates and glycoproteins have been well investigated,15-17 the mechanisms associated with the enhanced affinities of lectins binding to multivalent carbohydrates and glycoproteins have been less well investigated until recently.18,19... 

Importantly, the internal diffusion model for lectins binding to mucins is distinct from the classical lock and key model of ligand binding to a receptor. The internal... 

The deprotected lactosides were evaluated as inhibitors against lectin binding in a solid-phase inhibition assay with immobilized ASF on the surface of microtiter plate wells, mimicking cell-surface presentation, while mammalian galectins-1, -3, and -5 were in solution. Strong multivalency effects and selectivity were observed for the... 

S. Andre, B. Liu, H.-J. Gabius, and R. Roy, First demonstration of differential inhibition of lectin binding by synthetic tri- and tetravalent glycoclusters from cross-coupling of rigidified 2-propynyl lactoside, Org. Biomol. Chem., 1 (2001) 3909-3916. 

G. M. L. Consoli, F. Cunsolo, C. Geraci, and V. Sgarlata, Synthesis and lectin binding ability of glycosamino acid-calixarenes exposing GlcNAc clusters, Org. Lett., 6 (2004) 4163 1166. 

F. Sansone, L. Baldini, A. Casnati, and R. Ungaro, Conformationally mobile glucosylthioureidocalix[6]- and calix[8]arenes Synthesis, aggregation and lectin binding, Supramol. Chem., 20 (2008) 161-168. 

I. Baussanne, J. M. Benito, C. Ortiz Mellet, J. M. Garcia Fernandez, and J. Defaye, Synthesis and comparative lectin-binding affinity of mannosyl-coated /i-cyclo-dextrin-dendrimer constructs, Chem. Commun. (2000) 1489-1490. 

F. J. Feher, K. D. Wyndham, and D. J. Knauer, Synthesis, characterization and lectin binding study of carbohydrate functionalized silsesquioxanes, Chem. Commun. (1998) 2393-2394. 

R. Roy and J. M. Kim, Cu(II)-Self-assembling bipyridyl-glycoclusters and dendrimers bearing the Tn-antigen cancer marker Syntheses and lectin binding properties, Tetrahedron, 59 (2003) 3881-3893. 

D. Zanini and R. Roy, Chemoenzymatic synthesis and lectin binding properties of dendritic /V-acetyllactosaniine, Bioconjug. Chem., 8 (1997) 187-192. 

E. K. Woller and M. J. Cloninger, The lectin-binding properties of six generations of mannose-functionalized dendrimers, Org. Lett., 4 (2002) 7-10. 

Kimura, A., Orn, A., Holmquist, G., Wizzell, H., and Ersson, B. (1979) Unique lectin-binding characteristics of cytotoxic T-lymphocytes allowing their distribution from natural killer cells and K cells. Eur. J. Immunol. 9, 575. 

---