Cells cell-adhesive protein

Lutz, K.L. Szabo,L. A. Thompson, D.L. Siahaan, T. J., Antibody recognition of peptide sequence from the cell-cell adhesion proteins N- and E-cadherins, Peptide Res. 9, 233-239 (1996). 

Tight junctions form selective barriers that regulate paracellular transport across epithelia and endothelia. Tight junctions are composed of transmembrane proteins (occludin, claudins, and JAMs) linked to the actin cytoskeleton through cytoplasmic ZO proteins, whereas adherence junctions are composed of the nectin-afadin system and the E-cadhcrin catenin system [143]. These cell cell adhesion proteins create the barrier and regulate electrical resistance, size, and ionic charge selectivity [144], Several experimental procedures have... 

Recent studies from our laboratory demonstrate an essential role for the p21 activated kinase (Pak) 1 in control of salivary gland lumen size through the cell-cell adhesion protein, E-cadherin. Pak proteins are serine-threonine kinases that control vascular integrity in zebrafish blood vessels (Buchner et al., 2007 Liu et al., 2007) and lumen formation by human endothelial cells cultured in three-dimensional collagen matrices (Koh et al., 2008 Koh et al., 2009). In the Drosophila embryonic salivary gland, Pakl functions downstream of the small... 

Flavonoids inhibit cytiokine-indnced endothelial cell adhesion protein gene expression American Journal Patholology 147, 278-92. 

Figure 4c shows that the amount of adsorbed proteins is rapidly saturated within several minutes of exposing serum-containing medium to a surface. Albumin, the most abundant serum protein, was expected to preferentially adsorb onto the surfaces during early time points. Then, adsorbed albumin was expected to be displaced by cell adhesion proteins. To investigate the effect of preadsorbed albumin displacement on cell adhesion, SAMs were first exposed to albumin then, HUVECs suspended in a serum-supplemented medium were added [21, 42]. Very few cells adhered to hydrophobic SAMs that had been pretreated with albumin, due to the large interfacial tension between water and the hydrophobic surfactant-like surface. Albumin was infrequently displaced by the cell adhesive proteins Fn and Vn. One the other hand, HUVECs adhered well to hydrophilic SAM surfaces that had been preadsorbed with albumin. In that case, the preadsorbed albumin was readily displaced by cell adhesive proteins. 

Anchoring junctions between cells involve cell adhesion proteins known as cadherins which are tissue specific, so that cells normally adhere only to similar cells. In tissues that require enhanced mechanical strength, special points of attachment exist where microfilaments in adjacent cells are linked to opposed arrays of cadherins. 

Cell adhesion protein (intercellular adhesion molecule) lCAM-1 is up-regulated. This protein plays a role in cell-cell adhesion and leukocyte migration. 

In contrast, through eons of evolution Nature has come up with many biopolymers that can combine important mechanical properties including strength, toughness, and elasticity. For example, sUks (Oroudjev et al. 2002), cell adhesion proteins (Law et al. 2003), and connective proteins existing in both soft and hard tissues such as muscle (Kellermayer et al. 1997 Rief, Gautel, et al. 1997 Marszalek et al. 1999 Li et al. 2000), seasheUs (Smith et al. 1999), and bone (Thompson et al. 2001)... 

Kussmann, M. et al.. Matrix-assisted laser desorption/ionization mass spectrometric peptide mapping of the neural cell adhesion protein neurolin purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis or acidic precipitation.. Mass Spec., 32, 483,1997. 

Here are two of many known examples of specific cell-cell adhesion. The species-specific reaggregation of dissociated cells of marine sponges (Chapter 1) depends upon a 20-kDa proteoglycan of unique structure209-211 together with a cell surface receptor protein and calcium ions. The recognition of egg cell surfaces by sperm212-214 is species... 

Gregonis et al. have shown that PEO bound to quartz surfaces greatly minimizes the adsorption of proteins from plasma and from singly component protein solutions 122). Bell and coworkers at Los Alamos have developed a theory of cell-cell adhesion based on the steric repulsion characteristics of the hydrophilic macromolecules present on cell surfaces 124). 

Additional Cell Adhesion Proteins Are Required to Mediate the Immune Response Immune Recognition Molecules Are Evolutionarily Related... 

Mucus. The cells of internal epithelia throughout the body are surrounded by an intercellular ground substance known as mucus. The principal components of mucus are complexes composed of proteins and carbohydrates. These complexes may be free of association or may be attached to certain regions on cell surfaces. This matrix may play a role in cell-cell adhesion, as well as acting as a lubricant, allowing cells to move relative to one another.99 Moreover, mucus is believed to play a role in bioadhesion of mucoadhesive drug delivery systems.100... 

Cadherins. Cadherins are a group of glycoproteins that facilitate Ca2+-dependent cell-cell adhesive interactions.20 When cadherin function is disrupted, the release of a tumor cell can result. It was shown that the aggressive metastasis of undifferentiated epithelial carcinoma cells that had lost cell-cell adhesion could be stopped by transfection with E-cad-herin cDNA.21 Therefore, it was suggested that E-cadherin suppresses metastasis,22 which was further supported by more recent studies that showed the loss of adhesion of human gastric, prostatic, and lung cancer cells was due to the gene mutation of a protein associated with the proper function of cadherin.23... 

Krasnoperov V, Bittner MA, Holz RW et al (1999) Structural requirements for a-latrotoxin binding and a-latrotoxin-stimulated secretion. A study with calcium-independent receptor of a-latrotoxin (CIRL) deletion mutants. J Biol Chem 274 3590-6 Krasnoperov V, Lu Y, Buryanovsky L et al (2002a) Post-translational proteolytic processing of the calcium-independent receptor of a-latrotoxin (CIRL), a natural chimera of the cell adhesion protein and the G protein-coupled receptor. Role of the G protein-coupled receptor proteolysis site (GPS) motif. J Biol Chem 277 46518-26... 

B2. Bains, R., Furness, P. N., and Critchley, D. R., A quantitative immunofluorescence study of glomerular cell adhesion proteins in proteinuric states. J. Pathol. 183, 272-280 (1997). 

Wood B, Leong AS-Y. Cell adhesion proteins - Biology, detection and applications. Pathology 2003 35 101-105. 

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