Cell-adhesive proteins

Flavonoids inhibit cytiokine-indnced endothelial cell adhesion protein gene expression American Journal Patholology 147, 278-92. 

Lutz, K.L. Szabo,L. A. Thompson, D.L. Siahaan, T. J., Antibody recognition of peptide sequence from the cell-cell adhesion proteins N- and E-cadherins, Peptide Res. 9, 233-239 (1996). 

Figure 4c shows that the amount of adsorbed proteins is rapidly saturated within several minutes of exposing serum-containing medium to a surface. Albumin, the most abundant serum protein, was expected to preferentially adsorb onto the surfaces during early time points. Then, adsorbed albumin was expected to be displaced by cell adhesion proteins. To investigate the effect of preadsorbed albumin displacement on cell adhesion, SAMs were first exposed to albumin then, HUVECs suspended in a serum-supplemented medium were added [21, 42]. Very few cells adhered to hydrophobic SAMs that had been pretreated with albumin, due to the large interfacial tension between water and the hydrophobic surfactant-like surface. Albumin was infrequently displaced by the cell adhesive proteins Fn and Vn. One the other hand, HUVECs adhered well to hydrophilic SAM surfaces that had been preadsorbed with albumin. In that case, the preadsorbed albumin was readily displaced by cell adhesive proteins. 

Anchoring junctions between cells involve cell adhesion proteins known as cadherins which are tissue specific, so that cells normally adhere only to similar cells. In tissues that require enhanced mechanical strength, special points of attachment exist where microfilaments in adjacent cells are linked to opposed arrays of cadherins. 

Cell adhesion protein (intercellular adhesion molecule) lCAM-1 is up-regulated. This protein plays a role in cell-cell adhesion and leukocyte migration. 

In contrast, through eons of evolution Nature has come up with many biopolymers that can combine important mechanical properties including strength, toughness, and elasticity. For example, sUks (Oroudjev et al. 2002), cell adhesion proteins (Law et al. 2003), and connective proteins existing in both soft and hard tissues such as muscle (Kellermayer et al. 1997 Rief, Gautel, et al. 1997 Marszalek et al. 1999 Li et al. 2000), seasheUs (Smith et al. 1999), and bone (Thompson et al. 2001)... 

Kussmann, M. et al.. Matrix-assisted laser desorption/ionization mass spectrometric peptide mapping of the neural cell adhesion protein neurolin purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis or acidic precipitation.. Mass Spec., 32, 483,1997. 

Additional Cell Adhesion Proteins Are Required to Mediate the Immune Response Immune Recognition Molecules Are Evolutionarily Related... 

Tight junctions form selective barriers that regulate paracellular transport across epithelia and endothelia. Tight junctions are composed of transmembrane proteins (occludin, claudins, and JAMs) linked to the actin cytoskeleton through cytoplasmic ZO proteins, whereas adherence junctions are composed of the nectin-afadin system and the E-cadhcrin catenin system [143]. These cell cell adhesion proteins create the barrier and regulate electrical resistance, size, and ionic charge selectivity [144], Several experimental procedures have... 

Krasnoperov V, Bittner MA, Holz RW et al (1999) Structural requirements for a-latrotoxin binding and a-latrotoxin-stimulated secretion. A study with calcium-independent receptor of a-latrotoxin (CIRL) deletion mutants. J Biol Chem 274 3590-6 Krasnoperov V, Lu Y, Buryanovsky L et al (2002a) Post-translational proteolytic processing of the calcium-independent receptor of a-latrotoxin (CIRL), a natural chimera of the cell adhesion protein and the G protein-coupled receptor. Role of the G protein-coupled receptor proteolysis site (GPS) motif. J Biol Chem 277 46518-26... 

B2. Bains, R., Furness, P. N., and Critchley, D. R., A quantitative immunofluorescence study of glomerular cell adhesion proteins in proteinuric states. J. Pathol. 183, 272-280 (1997). 

Wood B, Leong AS-Y. Cell adhesion proteins - Biology, detection and applications. Pathology 2003 35 101-105. 

Cell adhesion on a nonfunctional scaffold is mediated dominantly by nonspecific, entropically favored adsorption of a layer of cell adhesion proteins, excreted by the cell itself [61]. In order to obtain and retain the native function of these proteins, attempts are being made to tune the hydrophilicity or hydrophobicity of the scaffold surfaces [62], Different methods of surface activation are commonly applied, e.g., blending, copolymerization, plasma treatment, etching, radiation, chemical surface modification, coatings, and combinations of those. 

Cell adhesion, protein-mediated, on biomaterials, 143-145 Ceramics... 

Johansson MW, Lind MI, Holmblad T et al (1995) Peroxinectin, a novel cell adhesion protein from crayhsh blood. Biochem Biophys Res Commun 216 1079-1087... 

The significance of exon shuffling to protein evolution, in particular in respect to the development of multicellularity, is signified by a short inventory of processes involving proteins created by modular assembly. Exon shuffling facilitates the construction of proteins involved in regulation of blood coagulation, fibrinolysis, and complement activation, plus most constituents of the extracellular matrix, cell adhesion proteins, and receptor proteins [10, 57]. 

Cadherins are calcium-dependent cell adhesion proteins that mainly participate in interactions with cadherins, located on other cells. Cadherins have five similar extracellular domains some of which have calcium-binding sites, and an intracellular C-terminal domain that interacts with the actin cytoskeleton. Different cadherins are named according to the tissues were they were first found. E-cadherins were found in many epithelial cells. N-cadherins are present in nerves and muscle and P-cadherin are present in placental cells. 

Desmosomes are intercellular junctions, consisting of two cell membranes, separated by an interspace. The interspace contains dense fibrillar plaques rich in cell adhesion proteins of the cadherin type. Desmosomes are important for cell-cell adhesion and communication. Epithelial tissue in the body stretched mechanically is rich on desmosomes. 

Cadherins A group of cell adhesion proteins that enable cells to interact with other cells... 

Carrico IS, Maskarinec SA, Heilshom SC, Mock ML, Liu JC, Nowatzki PJ, Franck C, Ravichandran G, Tirrell DA. Lithographic patterning of photoreactive cell-adhesive proteins. J. Am. Chem. Soc. 2007. In press. 

Cellular differentiation marker 2, a cell adhesion protein found on the surface of T cells and natural killer cells... 

These studies provide a base for further development of collagen materials for specific biomaterial applications. Further studies are being directed to the effect of collagen film on blood in terms of platelet and white cell adhesion, protein absorption, and thrombogenicity. 

Lasky, L.A. (1991). Lectin cell adhesion molecules (LEC-CAMs) a new family of cell adhesion proteins involved with inflammation. J. Cell. Biochem. 45, 139-146. 

Hirao K, Hata Y, Ide N, Takeuchi M, Irie M, et al. 1998. A novel multiple PDZ domain-containing molecule interacting with N-methyl-D-aspartate receptors and neuronal cell adhesion proteins. J. Biol. Chem. 273 21105-10... 

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