Protein serum

When Yamakawa (Y2) studied the gastric juice proteins in regard to resistance to alkali, formalin, and tryptic digestion, he found that most were more resistant to these agents than serum proteins. He opposed, therefore, the concept that proteins of gastric juice are derived from serum in which, as we now know, he was only partly correct. 

Passage of P i-Labeled Albumin into Gastric Juice 

In 1957, Citrin et al. reported the flrst clear-cut demonstration of the passage of serum albumin into the gastric juice in a patient with Me- 

Ishimori in our laboratory injected Ii -labeled albumin i.v. into more than 20 patients vrith various gastric disease and controls to ascertain the passage of labeled albumin into the gastric juice at various time intervals after injection, extending up to 7 days. Methods used for 

Acetylcholine mucus Fluid portion (Heidenhoin pouch-doi 

The congealing temperature of the blood serum of polar fish of Arctic or Antarctic regions (e. g. Trematomus borchgrevinski, Dissostichus maw-soni, Boreogadus saida) is about — 2 °C and thus is significantly lower than that of other fish (—0.6 to — 0.8 °C). Antifreeze gly-coproteins account for such low values. The amino acid sequence of this class of proteins is characterized by high periodicity  

The molecular weight range is 10.5-27 kdal, while the conformation is generally stretched, with several (2-helical regions. These glycoproteins are hydrated to a great extent in solution. The antifreezing effects are attributed to the disaccharide residues as well as to the methyl side chains of the peptide moiety. 

The nonprotein-N content is 9-18% of the total nitrogen content in teleosts and 33-38% in elas-mobranchs. 

Guanidine compounds, such as creatine, are 600-700 mg/kg fresh fish muscle tissue. In crustaceans, the role of creatine is taken over by arginine. 

WiELAND and Determann [191] have separated lactate-dehydrogenase isoenzyme and AMP, ADP and ATP from one another on DEAE-Sephadex layers, using gradient elution chromatography (p. 90). 

Wagner-Romero [176] has been able to accomphsh separation of a serum into 15 fractions, using TL-starch gel electrophoresis. Reissell 

and co-workers [41] have separated the protein fractions in serum by thin-layer electrophoresis and compared those of healthy rats and of rats with tumours. Albumin and the a-globuhns were present in distinctly smaller amounts in the serum of rats with larger tumours. 

---

Dekker A, Reitsma K, Beugeling T, Bant]es A, Fei]en J and van Aken W G 1991 Adhesion of endothelial-oells and adsorption of serum-proteins on gas plasma-treated polytetrafluoroethylene S/omaferfa/s 12 130-8... 

Hydroxymethyl-6-methyluracil (1043) was prepared many years ago from 6-methyl-uracil and formaldehyde, or in other ways. Since 1956 it has received much attention in the USSR under the (transliterated) name pentoxyl or pentoxil. It is used in several anaemic and disease conditions. For example, a mixture of folic acid and pentoxyl quickly reduces the anaemia resulting from lead poisoning pentoxyl stimulates the supply of serum protein after massive blood loss it stimulates wound healing it stimulates the immune response in typhus infection and it potentiates the action of sulfonamides in pneumococcus infections (70MI21300). 

Serum proteins, e g albu- Allow to react at the start with 0 05% dansyl mm chlonde solution m hexane, dry and expose for... 

A. W. K. Tiselius (Uppsala) electrophoresis and adsorption analysis, especially for discoveries concerning the complex nature of the serum proteins. 

Decreased serum proteins Decreased renal mass, blood flow, and glomerular filtration rate... 

Figure 9.2. Amino acid content (mol %) in collagen extract (dark columns) and serum proteins (light columns) from a skeleton from coastal Peru. Due to a prevalence of degraded and soluble collagen, the nonmineral-bound protein fraction shows a collagen amino acid profile.

Figure 9.4. Non-proteinogenetic amino acids in NCP s from all archaeological human bones. The values are the averages of all archaeological human bone samples taken together (cf Table 9.1). Dark columns mineral-bound NCP light columns serum proteins. Most non-proteinogenic amino acids are extracted with the serum proteins.

Works where study the hydrodynamic properties of a biopolymers in aqueous solution at different temperatures are made by Guner (1999), and Guner Kibarer (2001) for dextran Ghen Tsaih (1998) and Kasaii (2008) for chitosan, Bohidar for gelatin (1998), and Monkos for serum proteins (1996,1997,1999, 2000, 2004 and 2005). 

Table 3 shows the classical values calculating for Mark-Houwink parameters, a and k for temperature. These studies on M-H parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited range of temperature (for gelatin Pouradier Venet 1954 and Bohidar 1998 Monkos for serum proteins 1996, 1997, 1999, 2000, 2004 and 2005). This value shows a clear functionality between these parameters and temperature. 

Separation methods, multichromato-graphic la 56 Serine la 246,356 lb 132 Serotonin la 70,76,239,240,262,355, 380 lb 37-39,231,243,348 Serotonin metabolites lb 327 Serum lipids la 89 Serum proteins la 74 Sesquiterpene derivatives lb 239,446 Sesquiterpene esters lb 239 Sesquiterpene glucosides la 327 Sesquiterpene lactones lb 448 Sevin lb 387-389 Si 50 000, specific surface area la 91 Silica gel, caffeine-impregnated la 85 -, surface modified la 3 Silica gel 60, specific surface area la 91... 

An area worthy of study is the development of systems of increasing sample throughput beyond the single column operation. Scott has introduced a prototype multicolumn system based on the centrifugal analyzer principle (53). In this set-up a series of LC colimns is rotated on a disc, with sample delivery at the center of the disc and elution and spectrophotometric analysis on the outside. He has suggested using affinity columns for rapid serum protein analysis by this approach. Of course, other principles, such as segmented flow, could be envisioned in an automated LC system as well. Undoubtedly, we can expect to see the availability of such systems in the next few years. 

Some drugs act as Indicators (e.g. phenolphthaleln, vegetable laxatives) and affect tests carried out at a particular pH. The presence of sulfobromophthaleln dye (BSP) In serum will Interfere with serum protein determined by the biuret method. 

Complement comprises a group of heat-labile serum proteins which, when activated, are associated with the destruction of bacteria in the body in a variety of ways. It is present in low concentrations in serum but, as its action is linked intimately with a second (specific) set of defence mechanisms, its composition and role will be dealt with later in the chapter. 

SHASHIKANTH K N, BASAPPA s c, SREENIVASA MURTHY V (1984) A Comparative study of raw garlic extract and tetracycline on caecal micro flora and serum proteins of albino rats. Folia Microbiol (Praha). 29 348-52. 

A definitive identification of the proteins in each peak is not possible, however, the elution times of the peaks at 13-14 min. and 15 min. are close to the times which would be expected for gamma-globulins and albumins, two of the principal classes of serum proteins. These data also indicate the loading capacity of this column with serum. More than 14 mg. of undiluted serum was injected before evidence of overloading in the form of band broadening and peak distortion was observed. 

Figure 3. Fractionation of human serum proteins on Spherogel TSKSW 3000, The conditions were as in Figure 1. The analyses were made using (A) a 50-fiL injection loop with an analytical flow cell (B) a 100-L loop with a semipreparative flow cell or (C,D) a 500-L loop with a preparative flow cell.

---