Deletion mutants

While wild-type PAMO was unable to convert 2-phenylcyclohexanone efficiently, all deletion mutants readily accepted this ketone as substrate. All mutants also displayed a similar thermostability when compared with the parent enzyme. The most active mutant (deletion of S441 and A442) was used for examining its enantioselective properties. It was found that the mutant preferably formed the (/ )-enantiomer of the corresponding lactone E = 100). While CHMO also shows a similar enantioselective behavior, this PAMO deletion mutant is a better candidate for future applications due to its superior stability. This clearly demonstrates that PAMO can be used as parent enzyme to design thermostable BVMO variants. It also illustrates that the available crystal structure of PAMO will be of great help for BVMO redesign efforts. ... 

Harder PA, DP O Keefe, JA Romesser, KJ Leto, CA Omer (1991) Isolation and characterization of Strepto-myces griseolus deletion mutants affected in cytochrome P-450-mediated herbicide metabolism. Mol Gen Genet 227 238-244. 

Keil H, PA Williams (1985) A new class of TOL plasmid deletion mutants in Pseudomonas putida MT15 and their reversion by tandem gene amplification. J Gen Microbiol 131 1023-1033. 

G. F. Maley, P. Van Roey 2001, (Crystal structure of a deletion mutant of human thymidylate synthase Delta(7-29) and its ternary complex with Tomudex and dUMP), Protein Sci. 10(5), 988. 

Babich, K., Engle, M., Skinner, J.S., and Laddaga, R.A., Deletion mutant analysis of the Staphylococcus aureus plasmid pI258 mercury-resistance determinant, Can J Microbiol, 37 (8), 624-631, 1991. 

Pan, Y. P. Daggett, V., Direct comparison of experimental and calculated folding free energies for hydrophobic deletion mutants of chymotrypsin inhibitor. 2 Free energy perturbation calculations using transition and denatured states from molecular dynamics simulations of unfolding, Biochemistry 2001,40, 2723-2731. 

PS1 The PS 1-prep, introduced in this communication is the first reported with a polyhistidine tag fused to the N-terminus of the PsaF subunit. This construct was possible due to the fact that cyanobacterial PsaF-deletion mutants show no impact on photoautotrophic growth - in contrast to Chlamydomonas reinhardtii, where inactivation of PsaF results in a severe reduction of electron transfer from plastocyanin to PS 1 [Hippier et al. 1997], Also, the N-terminus of the F-subunit which was decorated by the tag is located towards the lumen side which enables an attachment of the isolated PS1 with the lumen-exposed /donor-side to the electrode surface in our hydrogen-producing device. 

Reyes-Cruz, G., Hu, J., Goldsmith, P. K., Steinbach, P. J., and Spiegel, A. M. (2001) Human Ca2+ receptor extracellular domain. Analysis of function of lobe I loop deletion mutants../. Biol. Chem. 276, 32145-32151. 

The rotational dynamics of ethidium intercalated in double-strand DNAs of intact bacteriophages, namely, X, the deletion mutant XA, T4D (wild type), and T4dC (with normal cytosine instead of glucosylated hydroxymethyl-... 

Although CSN is not essential in yeast, the csnl and csn2 S. pombe deletion mutants display slow growth and sensitivity to UV- and y-irradiation [52]. Other csn mutants did not show significant phenotypes apart from the loss of culhn s dened-... 

Enzyme Function Main expression pattern Phenotype of homozygous deletion mutants... 

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