Cancer tumor cell lines

Recent observations suggest that carotenoids may modulate the AP-1 activation process. It has been recently reported in mammary tumor cell lines that [3-carotene and its cleavage products were able to decrease the activation of AP-1 (Tibaduiza et al., 2002). Moreover, lycopene was also shown to downregulate AP-1 in mammary cancer cells (Karas et al., 2000). In addition, a pharmacological... 

Fogh, J., J. M. Fogh, and T. Orfeo. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice, J. Natl. Cancer Inst. 1977, 59, 221-226... 

Maliepaard M, van Gastelen MA, de Jong LA, Pluim D, van Waardenburg RC, Ruevekamp-Helmers MC et al. Overexpression of the BCRP/MXR/ABCP gene in a topotecan-selected ovarian tumor cell line. Cancer Res 1999 ... 

MacLeod RA, Dirks WG, Matsuo Y, Kaufmann M, Milch H, Drexler HG. Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int. J. Cancer 1999 83 555-563. 

When used in tumor cells, fulvestrant was initially described as a potent, competitive growth inhibitor of ER-positive, human breast cancer MCF-7 cells, whose growth is stimulated by estradiol. The compound was ineffective in tumor cell lines without ER, such as MDA-MB-231. The inhibitory effects were more pronounced with fulvestrant than with tamoxifen in the same cell line (Wakeling et al. 1991). 

Epothilones A, B and E (4,5 and 6) (Fig. 2) are representative members of a new class of bacterially derived natural products which exhibit potent biological activity. Isolated by Hofle and coworkers [6] from a soil sample collected near the Zambesi river, the compounds have provided a great deal of excitement in the scientific community due to their potent cytotoxicity against a number of multiple drug-resistant tumor cell lines and because of the mechanism by which they exert this effect. Like Taxol [7], the epothilones promote the combination of a- and 3-tubulin subunits and stabilize the resulting microtubule structures. This mode of action inhibits the cell division process and is, therefore, an attractive strategy for cancer chemotherapy [7,8]. 

Harker, W.G., et al., Human tumor cell line resistance to chemotherapeutic agents does not predict resistance to natural killer or lymphokine-activated killer cell-mediated cytolysis, Cancer. Res. 50, 18, 5931, 1990. 

Lieber M, Smith B, Szakal A, Nelson-Rees W, Todaro G (1976) A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int J Cancer 17(1 ) 62—70... 

Delaney CA, Wang LZ, Kyle S, White AW, Calvert AH, Curtin NJ, Durkacz BW, Hostomsky Z, Newell DR (2000) Potentiation of temozolomide and topotecan growth inhibition and cytotoxicity by novel poly(adenosine diphosphoribose) polymerase inhibitors in a panel of human tumor cell lines. Clin Cancer Res 6 2860-2867... 

Alley, M. C., Scudiero, D. A., Monks, A., Hursey, M. L., Czerwinski, M. J., Fine, D. L., Abbott, B. J., Mayo, J. G., Shoemaker, R. H., and Boyd, M. R. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetra-zolium assay. Cancer Res., 1988, 48, 589-601. 

Moved] Cranberry fruit of Early Black cultivar was fractionated chromatographically and fractions were analyzed for flavonoid content. The effects of the flavonoid fractions and ursolic acid, an abundant triterpenoid in cranberry peel, were assessed in two models of colon cancer and one model of breast cancer. Clonogenic soft agar assays were used to determine the effect of these compounds on tumor colony formation in HCT-116, HT-29 and MCF-7 cells. MTT and trypan blue assays were performed to assess their ability to inhibit tumor cell proliferation. TUNEL assays were performed to assess apop-totic response to the cranberry compounds. The proanthocyanidins inhibited tumor colony formation in HCT-116 and HT-29 cells in a dose-dependent manner, with greater effect on the HCT-116 cell line. Ursolic acid strongly inhibited tumor colony formation in both colon cell lines. These compounds also decreased proliferation in all three tumor cell lines with the HCT-116 cell line most strongly affected. (150 words)... 

Roche has reported a 2,4-diaminopyrimidine 42 (Ro-4584820) to be a Phase I clinical candidate [48,103]. Compound 42 is a pan-CDK inhibitor (CDKl K = 0.001 ptM CDK2 K[ = 0.003 p.M CDK4 K[ = 0.001 p.M), consequently it induces both G1 and G2 cell cycle arrest in tumor cell lines. It is a potent inhibitor of tumor cell proliferation in both the HCT-116 colon cancer (IC50 = 0.080 tiM) and the H460A (IC50 = 0.055 p.M) lung cancer cell lines. 

Dean N, McKay R, Miraglia L, Howard R, Cooper S, Giddings ], Nicklin P, Meister L, Ziel R, Geiger T, Muller M, Fabbro D (19 ) Iidiibition of growth of human tumor cell lines in nude mice by an antisense oligonucleotide inhibitor of protein kinase C-a expression. Cancer Res 56 3499-3507 Defranco AL (1991) Immunosuppressants at work. Nature 352 754-755 De las Alas MM, Aebi S, Fink D, Howell SB, Los G (1997) Loss of DNA mismatch repair effects on the rate of mutation to drug resistance. J Natl Cancer Inst 89 1537-1541... 

Fitzsimmons SA, Workman P, Grever M, Pauli K, Camalier R, Lewis AD (1996) Reductase enzyme expression across the National Cancer Institute tumor cell line panel correlation with sensitivitry to mitomycin C and E09. J Natl Cancer Inst 88 259-269... 

Armstrong PB, Quigley JP, Sidebottom E (1982) Transepithelial invasion and intrames-enchymal infiltration of the chick embryo chorioallantois by tumor cell lines. Cancer Res 42 1826-1837... 

Teicher BA, Holden S A, Herman TS, et al. Characteristics of five human tumor cell lines and sublines resistant to cis-diamminedichloroplatinum(II). Int J Cancer 1991 47 252-260. 

The success of taxane-based therapy in cancer treatment has stimulated interest in further improving its efficacy profile and in identifying new tubulin-active agents. Two taxane analogs, BMS-184476 and BMS-188797, demonstrate greater activity than paclitaxel or docetaxel in a number of human tumor cell lines as well as in rodent solid tumors and human xenografts (4,145). BMS-184476 was discovered to be more potent... 

Schwartz JL, Rotmensch J, Beckett MA, et al. X-ray and cis-diamminedichloroplatinum (II) crossresistance in human tumor cell lines. Cancer Res 1988 48 5133-5135. 

Bernhard EJ, McKenna WG, Hamilton AD, et al. Inhibiting Ras prenylation increases the radiosensitivity of human tumor cell lines with activating mutations of ras oncogenes. Cancer Res 1998 58 1754-1761. 

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