Bacteria bioluminescent

Daubner, S. C., and Baldwin, T. O. (1989). Interaction between luciferase from various species of bioluminescent bacteria and the Yellow Fluorescent Protein of Vibrio fischeri strain Y-l. Biochem. Biophys. Res. Commun. 161 1191-1198. 

Johnston, T. C., et al. (1990). The nucleotide sequence of the luxA and luxB genes of Xenorhabdus luminescence HM and a comparison of the amino acid sequences of luciferases from four species of bioluminescent bacteria. Biochem. Biophys. Res. Commun. 170 407- 115. 

Petushkov, V. N., Gibson, B. G., Visser, A. J. W. G., and Lee, J. (2000). Purification and ligand exchange protocols for antenna proteins from bioluminescent bacteria. Method. Enzymol. 305 164-180. 

Ecotoxicity assessment of water samples was carried out, in parallel to chemical analysis, using three standardized bioassays based on the micro-crustacean Daphnia magna, the algae Pseudokirchneriella subcapitata, and the bioluminescent bacteria Vibrio fischeri. 

The only illumination comes from the green and red lights emitted by the bioluminescent bacteria coating the trees. It reminds you of Christmas. 

Meighen, E. A. (1988). Enzymes and genes from the lux operonsof bioluminescent bacteria. Annual Review of Microbiology, 42, 151-76. 

A bay on the island of Vieques, off the coast of Puerto Rico, is famous for the bioluminescent bacteria it contains, which luminesce when they are disturbed by motion. The bacteria contain one of the varieties of compounds called luciferin. A model compound for the generation of chemiluminescence is luminol, shown superimposed here, which luminesces in the presence of hydrogen peroxide. 

Sweet, L.I., Travers, D.F. and Meier, P.G. (1997) Short Communication-chronic toxicity evaluation of wastewater treatment plant effluents with bioluminescent bacteria a comparison with invertebrates and fish, Environmental Toxicology and Chemistry 16 (10), 2187-2189. 

Bulich, A.A., Green, M.W. and Underwood, S.R. (1992) Measurement of soil and sediment toxicity to bioluminescent bacteria when in direct contact for a fixed time period. Abstract at Water Environment Federation, 65th Annual Conference and Exposition, September 20-24, New Orleans, LA. 

Bioluminescent bacteria are exceptionally sensitive, and even trace quantities of toxins in water reduce the amount of light they emit. In 1979, this phenomenon was used to assess the degree of pollution in water samples, the intensity of the bioluminescent light of these bacteria being measured.34 A year later, the American company AZUR Environmental (formerly Microbics Corporation) manufactured the first specialist equipment—Microtox —for carrying out analyses of environmental samples. 

Nowadays, Microtox is the most popular bioassay available that uses bioluminescent bacteria as its active element. Analysts find it a useful tool for assessing pollution in different compartments of the environment, possessing as it does both the advantages of bioindicator techniques and the precision of classic instrumental analysis. Here are some examples of its application ... 

Research on toxicity using bioluminescent bacteria can be conducted in two ways. Table 9.2 lists the types of information obtainable, depending on the form of the assay. 

The observed toxicity to bioluminescent bacteria was strictly correlated with the 89... 

Warne, M.S., Boyd, M.A., Meharg, E.M., Osbom, D., Killham, D., Lindon, J.C. and Nicholson, J.K. (1999a). Quantitative Stmcture-Toxicity Relationships for Halobenzenes in Two Species of Bioluminescent Bacteria, Pseudomonas fluorescens and Vibrio fischeri. Using an Atom-Centered Semi-Empirical Molecular-Orbital Based Model. SAR QSAR Environ.Res., 10, 17-38. 

Lee et al. built a cell-based array [93] which used 20 recombinant bioluminescent bacteria having different promoters fused with the bacterial Lux genes to detect and classify environmental toxicity. About 2 pi of the cell-agar mixture was deposited into the wells of a cell chip made of a 1 mm thick acryl plate with 96 1.2mm diameter wells. The chip was immersed into a solution... 

Toxicity screening of complex mixtures following separation on a HPTLC plate is a new technique invented by Bayer. Now ChromaDex (see Section 12.5 for the address) has incorporated this technology into a commercial kit (Bioluminex ) utilizing the bioluminescent bacteria. Vibrio fischeri. CAMAG has marketed the BioLumini-zer for the detection and documentation (see Section 8.8). 

Toxicity Screening Using the Bioluminescent Bacteria Vibrio fischeri... 

Toxicity screening follows the conventional TLC development and analysis process, which has to be performed in advance to separate possible toxic substances on the HPTLC plate. The developed and dried plate is dipped into a suspension of bioluminescent bacteria. Vibrio fischeri, thereby exposing any separated bioactive compounds to the test orgartisms. The luminescent activity is reduced or stopped by substances toxic to the bacteria, which may also be toxic to humans. Healthy Vibrio fischeri, emit very weak tight of greenish color, which can not be detected with standard systems using daylight cameras. Thus, a specific bioluminescence detection system is required. 

Hastings JW, Potrikus CJ, Gupta SC, KurfOrst M, Makemson JC. Biochemistry and Physiology of bioluminescent bacteria. Adv Microbiol Physiol 1985 26 235-91. 

Hastings J, Potrikus C, Gupta S, Kurfurst M, Makemson J. Biochemistry and physiology of bioluminescent bacteria. Advan Microb Physiol 1985 26 235-91. 

Hastings JW, Mitchell G. Endosymbiotic bioluminescent bacteria from the light organ of pony fish. Biol Bull 1971 141 261-8. 

Arfsten DP, Davenport R, and Schaffer DJ, Reversion of bioluminescent bacteria (Mutatox ) to their luminescent state upon exposure to organic compounds, munitions, and metal salts, Biomed. Environ. Sci., 7, 144, 1994. 

Nealson K, Hastings JW. Quorum sensing on a global scale massive numbers of bioluminescent bacteria make milky seas. Appl Environ Microbiol 2006 72 2295-7. 

Sellars, K.E. and Ram, N.W., Toxicity Assessment of Binary Metal Mixtures Using Bioluminescent Bacteria , University of Massachusetts, 1985. 

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