Cell chip

Fig. 18b.1. Electrochemical cells and representative cell configurations, (a) Schematic diagram of a cell-potentiostat system, (b) Typical laboratory cell with Hg-drop electrode and drop knocker, (c) Voltammetric cell as detector at the end of a high-performance liquid chromatographic column, (d) A two-electrode (graphite) chip cell for biosensor development, (e) Three-electrode chip cells on a ceramic substrate for bioanalytical work.

Matsubara, Y., Murakami, Y., Kobayashi, M., Morita, Y., and Tamiya, E. (2004). Application of on-chip cell cultures for the detection of allergic response. Biosens. Bioelectron. 19, 741-747. 

Cell retention, manipulation, and subsequent cellular analysis can be all achieved on-chip. Cell retention can be achieved by using slit- or weir-type filters, or by cell adhesion. In addition, fluid flow optical trapping and dielectrophoresis (DEP) have been exploited to manipulate and retain cells. 

Ichiki, T., Ujiie, T., Hara, T., Horiike, Y., Yasuda, K., On-chip cell sorter for single cell expression analysis. Micro Total Analysis Systems, Proceedings 5th a7AS Symposium, Monterey, CA, Oct. 21-25, 2001, 271-273. 

Wang L, Zhu J, Deng C et al (2008) An automatic and quantitative on-chip cell migration... 

Nanobarcodes Nanoemulsions Nanofibers Nanoparticles Nanoshells Carbon nanotubes Quantum dots Artificial binding sites Artificial antibodies Artificial enzymes Artificial receptors Molecularly imprinted polymers Cell simulations and cell diagnostics Cell chips Cell stimulators... 

Takahashi K, Hattori A, Suzuki I, Ichiki T, Yasuda K (2004) Non-destructive on-chip cell sorting system with real-time microscopic image processing. J Nanobiotechnology 2 5-12... 

Shlrasakl Y, Tanaka J, Makazu H, Tashlro K, Shoji S, Tsukita S, Funatsu T (2006) On-chip cell sorting system using laser-induced heating of a thermoreversible gelation polymer to control flow. Anal Chem 78(3) 695-701... 

Recent literature has provided descriptions of several devices that have integrated sample processing steps, such as on-chip cell lysis, cell selection, DNA purification, and PCR amplification. In one of... 

As a more complex example of integrated sample processing, Lagally et al. developed a device capable of dielectrophoretic cell sorting and on-chip cell lysis, followed by sequence-specific... 

Cell culture on a chip On-chip cell culture... 

In 2D cell culture on chip, cell adhesion has been extensively studied on patterned surfaces for it is critical to cellular functions. Micropattems have been used to study the cellular interactions with various materials such as metals, polymers, self-assembled monolayers, extracellular matrix proteins, cell adhesion peptides, and other bioactive molecules. The physical and chemical properties of a substrate affect the attachment and growth of cells on it, and many studies have demonstrated that different topographical features of a surface affect cell attachment. Glass, sflicon, and polydimethylsiloxane (PDMS) are widely used as substrate materials of ceU culture microchips. 

Cheung KC, Renaud P (2006) BIOMEMS for medicine on-chip cell characterization and implantable microelectrodes. Solid-State Electronics 50 551-557... 

Cell Culture (2D and 3D) on Chip Cell Patterning on Chip... 

Caspases are intracellular proteases and cleave their substrate proteins specifically behind an aspartate residue. Caspases are normally present as inactive proenzymes. However, during apoptosis, caspase activation is induced by cleavage at specific internal aspartate residues. Activation of the initiator caspases by pro-apoptotic signals leads to proteolytic activation of the execution caspases, which cleave a set of vital proteins and thus initiate and execute the apoptotic degradation phase. A self-contained on-chip cell culture and pretreatment microdevice system has been developed for the screening of caspase-3 expression in apoptotic cells. 

Tabuchi M, Baba Y (2004) Self-contained on-chip cell culture and pretreatmrmt system. J Piotermie Res 3 871-877... 

Cell lysis is the process of releasing intercellular biological molecules into the extracellular milieu by disrupting the cellular membrane. On-chip cell lysis is the process of lysing acellular sample on a biochip. 

On-chip cell lysis is a crucial component of integrated micro total analysis systems (pTAS). In order to perform biochemical analysis of intercellular molecules (i.e., proteins, lipids, and nucleic acids), the cells at first have to be disrupted, releasing the biomolecules from inside... 

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