Toxicity Screening Using the Bioluminescent Bacteria Vibrio fischeri

1 Toxicity Screening Using the Bioluminescent Bacteria Vibrio fischeri 

Toxicity screening follows the conventional TLC development and analysis process, which has to be performed in advance to separate possible toxic substances on the HPTLC plate. The developed and dried plate is dipped into a suspension of bioluminescent bacteria. Vibrio fischeri, thereby exposing any separated bioactive compounds to the test orgartisms. The luminescent activity is reduced or stopped by substances toxic to the bacteria, which may also be toxic to humans. Healthy Vibrio fischeri, emit very weak tight of greenish color, which can not be detected with standard systems using daylight cameras. Thus, a specific bioluminescence detection system is required. 

The RinT.iiminizer has been optimized for detection of bioluminescence patterns on HPTLC plates. The most important features are  

The CAMAG BioLuminizer stands out from similar system with its very smart and compact setup enabling precise and convenient positioning of the HPTLC plate, easy cleaning and compatibility with use imder field conditions. 

Fignre 104. Bioinminescence response for different toxicity levels 

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