Vibrio fischeri strain

Cho, K. W., Colepicolo, P., and Hastings, J. W. (1989). Autoinduction and aldehyde chain-length effects on the bioluminescent emission from the yellow protein associated with luciferase in Vibrio fischeri strain Y-lb. Photochetn. Photobiol. 50 671-677. 

Daubner, S. C., and Baldwin, T. O. (1989). Interaction between luciferase from various species of bioluminescent bacteria and the Yellow Fluorescent Protein of Vibrio fischeri strain Y-l. Biochem. Biophys. Res. Commun. 161 1191-1198. 

Karatani, H., and Hastings, J. W. (1993). Two active forms of the accessory yellow fluorescence protein of the luminous bacterium Vibrio fischeri strain Yl. J. Photochem. Photobiol., B 18 227-232. 

Hajime K, Yoshizawa S, Hirayama, S. Oxygen triggering reversible modulation of Vibrio fischeri strain Y1 bioluminescence in vivo. Photochem Photobiol 2004 79 120-5. 

Karatani H, Chiba T, Hirayama S. Relationship between spectral distribution of Vibrio fischeri strain Y1 bioluminescence and intracellular level of its fluorescent proteins. In Stanley PE, Kricka LJ editors. Bioluminescence and Chemiluminescence Progress and current applications. New Jersey World Scientific Publishing Co. 2002, 81-4. 

Test organism Vibrio fischeri, strain M169. 

The Microtox test system utilizes a strain of naturally occurring luminescent bacteria - Vibrio fischeri. Exposure to a toxic substance causes a disruption of the respiratory process of the bacteria resulting in reduced light output. The effective concentration (EC50) is determined as the concentration of a toxicant that causes a 50% reduction in light output over a prescribed period of time (typically 5, 15, or 30 min). The test is fast, fairly simple to conduct, uses small sample sizes, and is relatively inexpensive. Results correlate well with those from other toxicity bioassays such as fish and Daphnia. The test is used... 

Transgenic bacterial biosensors. Systems such as the Microtox assay detailed earlier use the marine species Vibrio fischeri as the sensor. Because it uses a marine bacterium, Microtox must be conducted in saline solution, which is ecologically irrelevant for most soils. Because no naturally luminescent soil bacteria are known that could be used as an alternative, one solution is to fuse the genes responsible for bioluminescence into soil-dwelling strains using recombinant technology (Paton et al., 1997). Two approaches can be used ... 

Mutatox test strain M-169 is a dim variant of Vibrio fischeri [45,46] and the primary genetic lesion responsible for the low light of this strain has not been completely identified. Current data support the assumption that the groBSL activity, a critical component of the lux regulatory system, is altered in this strain. A current model of this regulatory system is discussed in a recent paper by Adar et al. [47] and has been outlined by Richardson [48]. Further details can be found in the literature [47-51]. 

The use of bioluminescence has resulted in many different tests, including the use of naturally bioluminescent bacteria (27) aside from Microtox . The genes from the bacterium Vibrio fischeri, and other bioluminescent bacteria, have been cloned into bacteriophage (15) and into plasmids that are functional in a wide range of bacterial hosts, including E. coli and strains of Pseudomonas (3,21,22,29). Many different bacterial strains have been constructed with these plasmids and produce the lux or luc (fire-fly) genes, and, therefore, light, either under constitutive expression or by some method of induction. These bioluminescent bacterial strains have been used in... 

Abbreviations for cell strains are as follows Vh, Vibrio harveyi Vf, Vibrio fischeri Plu, Photorhabdus luminescens. Pie, Photobacterium leiognathi Pp, Photobacterium phosphoreum. The bacterium strain is followed by the designation of the luciferase subunit. Active luciferase heterodimers are indicated by ++ for the native enzymes and + for the hybrid enzymes. Inactive hybrids are indicated by the negative sign. 

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