U937 cells

Baird, S.K. et al., OxLDL induced cell death is inhibited by the macrophage synthesised pterin, 7,8-dihydroneopterin, in U937 cells but not THP-1 cells, Biochim. Biophys. Acta 1745, 361, 2005. 

A2. Aderka, D., Le, J., and Vilcek, J., IL-6 inhibits lipopolysaccharide-induced tumor necrosis factor production in cultured human monocytes U937 cells, and in mice. J. Immunol. 143, 3517-352) (1989). 

Dasatinib (Sprycel ) 12 inhibits Btk (IC50 = 5 nM) as well as the basal and lipopolysaccharide (LPS)-induced secretion of TNFa and IL-6 in U937 cells [65]. However, dasatinib s promiscuity [66] makes it difficult to ascribe these functional effects to direct Btk inhibition. The dasatinib-Btk(Y551E) crystal structure indicates binding to an active conformation, similar to that observed with Abl [43]. LFM-A13,13, was first described as a Btk inhibitor more than 10 years ago [67], although its poor Btk activity (IC50 = 2.5-17 /iM) and selectivity [68] require that caution be taken in interpreting functional effects. 

Iwama, K. et al., Apoptosis induced by arsenic trioxide in leukemia U937 cells is dependent on activation of p38, inactivation of ERK and the Ca2+-dependent production of superoxide, Int. J. Cancer, 92, 518, 2001. 

DCCD and vanadate. This indicates an active process energised by ATP. Ferrozine inhibition of Fe2+ and Fe3+ uptake implied the concerted involvement of both a Fe3+ reductase and FeoB in the uptake of iron supplied as Fe3+. It is concluded that FeoB-mediated Fe2+ represents a major pathway for H. pylori iron sequestration [189], In addition, growth experiments on the human pathogen L. pneumophila using artificial media, as well as replication studies within iron-depleted Hartmannella vermiformis amoebae and human U937 cell macrophages, provided evidence that the FeoB transporter is important for extracellular growth and intracellular infectivity [190],... 

Greenblatt, M. C., Elias, L. (1992). The type B receptor for tumor necrosis factor- a mediates DNA fragmentation in HL-60 and U937 cells and differentiation in HL-60 cells. Blood 80, 1339-46. 

Cell lines U937 (human lymphoblastoid cells) and T-98G (human astrocytoma) were obtained from the collection of cell lines at the Influenza Research Institute. T-98G cells were grown on a DMEM medium with the addition of 10% calf serum, and U937 cells onRPMI1640 (Biolot, St. Petersburg) with 10% calf serum. 

Calf serum was added to the medium for cell culture to 10% concentration. T98G or U937 cells were seeded onto 25 cm2 flasks for cell culture (Nunc, Denmark). Cells were cultivated for 72 h without serum, with 10% of intact serum or 10% serum after 6 h of photodynamic treatment. After the incubation period cells were harvested with chymotrypsine and their number was calculated in a hemocy-tometer chamber. 

B. Bauvois, J. Sanceau, J. Wietzerbin, Human U937 Cell Surface Peptidase Activities Characterization and Degradative Effect on Tumor Necrosis Factor-a , Eur. J. Immunol. 1992, 22, 923-930. 

One of the first reports showing that DAG attenuates ceramide induced apoptosis used two human myeloid leukemia cell lines, namely HL-60 and U937. Exposure of HL-60 and U937 cells to SMase or lOpM ceU-permeable ceramides for 6 h resulted in DNA-laddering, a typical hallmark of apoptosis. This could be aboUshed by di-octanoylglycerol, a cell permeable... 

Human fibroblast Collagenase (HFC) 57.000 (glycosylated) 52.000 0.32 Type 1, II, III, VII and X collagens gelatins a-Ms casein fibroblasts keratinocytes macrophages U937 cells endothelial cells [27,34]... 

Fig. 12.3 The +294T/C polymorphic site in PPARD influences the binding of transcription factor Spl (24). Electromobihty shift assays (EMSAs) of nnclear extract derived from hnman monocytic U937 cells using double-stranded 25-mer oligonucleotides corresponding to the sequence from position -1-281 to -1-305 of the common T allele and the rare C allele. Arrow refers to the -I-294C allele-predominant factor. F denotes free DNA. Lane 1, without extract lanes 2 to 4,12, with 10 ag nuclear extract in the absence of competitor lanes 5 to 7,13 to 15, with 10 ag nuclear extract in the presence of 150-fold excess of unlabeled DNA as competitor. Competitors used were -I-294C probe (lanes 5, 13 indicated with C) -I-294T probe (lanes 6, 14 indicated with T) and a nonspecific probe (lanes 7, 15 indicated with X). Spl complex. Lanes 8 to 11, with lOng nuclear extract in the presence of antibodies directed against Spl, p50, p65, and c-Rel, respectively...

Overexpression of PKC in U937 histiocytic lymphoma cells increased expression of PKCa and p isoforms. In response to TPA, parental U937 cells displayed growth arrest and differentiated into a monocyte/macrophage-like cell line, while PKC overexpressing cells underwent death. The abUity of GF109203X to inhibit TPA-induced cell death suggested that activation of a conventional isoform was necessary to induce apoptosis (de Vente et al., 1995). Activation of PKC enhanced and down-modulation of PKC activity reduced apoptosis of neuronal cells (Mailhos et al., 1994). Ceramide, an activator of PKC is an inducer of apoptosis (Muller et al, 1995 Hannun, 1998, Obeid et al., 1993). 

Whelan RD, Kiley SC, Parker PJ (1999) Tetradecanoyl phorbol acetate-induced microtubule reorganization is required for sustained mitogen-activated protein kinase activation and morphological differentiation of U937 cells. Cell Growth Diff 10 271-277... 

Summary of ligand, G protein, and receptor-binding data from previous studies with receptor proteins extracted from U937 cell membranes and solubilized in 1% DOM... 

The expression of iNOS and the generation of NO in LPS-activated RAW 264.7 cells were inhibited by resveratrol [Murakami et al., 2003 Tsai et al., 1999], The compound significantly diminished the expression of tumor necrosis factor (TNF)-o mRNA in LPS-activated J774.2 macrophage cells [Kowalski et al., 2005] and peripheral blood leukocytes [Richard et al., 2005]. The expression of interleukin (IL)-8 protein and/or its mRNA in human monocytic leukemia (U937) cells [Shen et al., 2003] and human peripheral blood... 

Li YT, Shen F, Liu BH, Cheng GF. 2003. Resveratrol inhibits matrix metalloproteinase-9 transcription in U937 cells. Acta Pharmacol Sin 24 1167-1171. 

To reduce cell adhesion during cell studies in microchips, various coating methods have developed. For instance, a polyacrylamide film was photopattemed on acrylate-coated glass, using 3-(trimethyoxysilyl)propylacrylate. This coating reduced the fraction of adhesion as follows CATH.a cells (1.05-0.25), U937 cells (0.99-0.11), and Jurkat T cells (0.05-0.04) [623],... 

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