U937 cell line

PythonF, Goebel C,AebyP (2007) Assessment of the U937 cell line for the detection of contact allergens. Toxicol Appl Pharmacol 220(2/113-124... 

Peck R, Bollag W (1991) Potentiation of retinoid-induced differentiation of HL-60 and U937 cell lines by cytokines. Eur J Cancer 27 53-57... 

McDevitt, TM, Tchao, R, Harrison, EH, and Morel, DW, 2005. Carotenoids normally present in serum inhibit proliferation and induce differentiation of a human monocyte/macrophage cell line (U937). J Nutr 135, 160-164. 

Cell lines U937 (human lymphoblastoid cells) and T-98G (human astrocytoma) were obtained from the collection of cell lines at the Influenza Research Institute. T-98G cells were grown on a DMEM medium with the addition of 10% calf serum, and U937 cells onRPMI1640 (Biolot, St. Petersburg) with 10% calf serum. 

One of the first reports showing that DAG attenuates ceramide induced apoptosis used two human myeloid leukemia cell lines, namely HL-60 and U937. Exposure of HL-60 and U937 cells to SMase or lOpM ceU-permeable ceramides for 6 h resulted in DNA-laddering, a typical hallmark of apoptosis. This could be aboUshed by di-octanoylglycerol, a cell permeable... 

Overexpression of PKC in U937 histiocytic lymphoma cells increased expression of PKCa and p isoforms. In response to TPA, parental U937 cells displayed growth arrest and differentiated into a monocyte/macrophage-like cell line, while PKC overexpressing cells underwent death. The abUity of GF109203X to inhibit TPA-induced cell death suggested that activation of a conventional isoform was necessary to induce apoptosis (de Vente et al., 1995). Activation of PKC enhanced and down-modulation of PKC activity reduced apoptosis of neuronal cells (Mailhos et al., 1994). Ceramide, an activator of PKC is an inducer of apoptosis (Muller et al, 1995 Hannun, 1998, Obeid et al., 1993). 

Although DC maturation can be used to detect sensitizing capacity, major concerns remain on this assay (i) the limited reproducibility within and between laboratories due to inter-donor variability and variations in cell isolation and culture techniques (ii) the lack of sensitivity and dynamic range [122]. To circumvent interdonor variability cell lines such as THP-1, U937, KG-1 and MUTZ-3 have been used. 

We have observed similar findings using U937, a monocytic cell line, and have detected EMP-monocyte complexes in patients with MS [67]. 

Twenty years ago, a high-affinity-binding site for uPA was demonstrated on the surface of peripheral blood monocytes and cultured cells of the human histiocytic lymphoma cell line, U937 [46]. The expression of uPAR on the cell surface of many cell types has since then been demonstrated, including a variety of neoplastic cell lines as well as nonneoplastic cells such as neutrophils, macrophages, keratinocytes, placental trophoblasts, endothelial, and smooth muscle cells [7, 33, 47-51]. The human uPAR gene has been mapped to chromosome 19ql.3 [52]. 

By use of mAbs with known domain specificity, a molecular variant of uPAR-lacking domain I, uPAR(II-III), was first identified on U937 cells [16]. Subsequently, uPAR(II-III) was identified on several neoplastic cell lines, in xenotransplanted human mammary and ovarian tumors as well as in tumor tissue lysates from ovarian cancer patients [20, 81-85], In solution, purified uPAR(I-III) can be cleaved in the linker region between domains I and II by... 

O Callaghan, Y.C., Woods, J.A., O Brien, N.M. 2002. Characteristics of 7 3-hydroxycholesterol-induced cell death in a human monocytic blood cell line, U937, and a human hepatoma cell line, HepG2. Toxicol, in Vitro 16, 245-251. 

Anderson CL, Abraham GN. Characterization of the Fc receptor for IgG on a human macrophage cell line, U937. J Immunol 1980 125(6) 2735-41. 

McCool D, Birshtein BK, Painter RH. Structural requirements of immunoglobulin G for binding to the Fc gamma receptors of the human tumor cell lines U937, HL-60, ML-1, and K562.J Immunol 1985 135(3) 1975-80. 

The human osteosarcoma cell line has been shown selectively to express COX-2 by reverse transcription-polymerase chain reaction and immunoblot analysis, whereas undifferentiated human lymphoma U937 cells... 

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