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Total alkaline phosphatase

Comment now is to be centered on the AutoAnalyzer total alkaline phosphatase method, using phenyl phosphate as substrate and the Powell and Smith aminoantipyrine-ferricyanide reagents, formulated in Fig. 3. [Pg.265]

This color reaction has now provided measurements of total alkaline phosphatase and the L-phenylalanine-sensitive isoenzyme. [Pg.265]

Our data support the statement that the heat sensitivity of each enzyme source remains characteristic and independent of the influence of the others in the mixture, and that the resultant heat inactivation is an additive function of the heat-sensitivities of members of the mixture. Bone enzyme from different sources is very consistently heat-sensitive (85-90%), unlike intestinal (50-65%), and liver enzyme (50-75%). However, the heat sensitivity of the LPSAP of normal serum can vary from 33 to 85% and of the non-LPSAP fraction from 50 to 95%. Therefore one cannot determine the identity of the organ sources of serum alkaline phosphatase with a knowledge of only the heat sensitivity and the total alkaline phosphatase. However, by correcting the heat-inactivation of serum by that contributed by intestinal component, one obtains the heat-inactivation of non-intestinal sources of alkaline phosphatase. If this value is 90% or more, the non-intestinal component could be presumed to be of osseous origin if 60% or less, of hepatic origin. [Pg.308]

Fig. 33. Effect of maintaining gastrointestinal secretion at 37°C as a function of time. Dotted line is the total alkaline phosphatase activity, (L) the activity in the presence of L-phenylalanine, and LPSAP the difference between D and L (D — L = LPSAP). Fig. 33. Effect of maintaining gastrointestinal secretion at 37°C as a function of time. Dotted line is the total alkaline phosphatase activity, (L) the activity in the presence of L-phenylalanine, and LPSAP the difference between D and L (D — L = LPSAP).
Fig. 36. Case A. Hyperphosphatasemia during androgen therapy in cancer of the endometrium. D-total = total alkaline phosphatase activity, non-LPSAP = activity in the presence of L-phenylalanine open circles, and n — l = LPSAP, closed circles. The same symbols apply to Figures 37, 38, and 39. Fig. 36. Case A. Hyperphosphatasemia during androgen therapy in cancer of the endometrium. D-total = total alkaline phosphatase activity, non-LPSAP = activity in the presence of L-phenylalanine open circles, and n — l = LPSAP, closed circles. The same symbols apply to Figures 37, 38, and 39.
Fishman et al. (F14) reported that cirrhotics with hyperphosphatasemia can be divided into two categories one in which the L-phenyl-alanine-sensitive moiety is increased, and one in which it is not. Careful clinical studies then and later (S49) have failed to reveal a correlation of the LPSAP with any one feature of the disease. On the other hand, a positive correlation was discovered between LPSAP and the ABO blood groups, subjects with blood types B and 0 exhibiting elevated LPSAP. On this evidence, our viewpoint now is that the genetic constitution of the individual is the factor that determines in hyperphosphatasemia the proportions of LPSAP of the total alkaline phosphatase. [Pg.340]

The biochemical fractions of the serum alkaline phosphatase in cirrhosis are listed in Table 13 according to blood types A and 0. It may be advantageous to compare the various moieties in these patients with the data on normal subjects of corresponding blood type (Table 10). In some cases (4, 5, 14) the elevation is proportionate in the LPSAP and non-LPSAP moieties, whereas in others (1, 2, 3, 9, 10, 12, 13, 15) there is a disproportionate elevation in the LPSAP fraction. Subject 7 exhibits the largest contribution of LPSAP (60%). There appears to be no similarity of the heat sensitivities of LPSAP and non-LPSAP in subjects 4, 5, 7, 14, and 15 with total LPSAP hyperphosphatasemia. In fact, the relatively high heat inactivation of sera 5, 6, 7, and 12 would register negatively for liver in a heat-inactivation test of total alkaline phosphatase (P19). [Pg.340]

Fig. 4. Serum alkaline phosphatase in 31 healthy women during the second and third trimesters of pregnancy. The continuous line represents the mean and the shaded area, the 2.5-97.5 percentiles. (A) represents total alkaline phosphatase and (B) the placental fraction. Most of the increase is due to the placental enzyme. From Romslo et al. (R26) with permis-... Fig. 4. Serum alkaline phosphatase in 31 healthy women during the second and third trimesters of pregnancy. The continuous line represents the mean and the shaded area, the 2.5-97.5 percentiles. (A) represents total alkaline phosphatase and (B) the placental fraction. Most of the increase is due to the placental enzyme. From Romslo et al. (R26) with permis-...
Matsushita, M., T. Mno, T. Kawaguchi, and T. Komoda. 2002. The effect of different buffers and amounts of intestinal alkalme phosphatase on total alkaline phosphatase activity. Clinica ChimicaActa 319 49-55. [Pg.35]

Mineral balance In a prospective, case-control study in 66 Indian patients with epilepsy, serum total alkaline phosphatase isoenzyme activities and calcium and 25-hydro xycolecalciferol concentrations... [Pg.110]

The cell growth was much faster on the chitosan-hydroxyapatite scaffolds with the glass than on the chitosan-hydroxyapatite scaffold without the glass. The total protein content of cells increased over time on both composites. The cells on the chitosan-hydroxyapatite-glass also expressed significantly higher amount of alkaline phosphatase at days 7 and 11 and osteocalcin at day 7 than those on chitosan-hydroxyapatite [165]. [Pg.172]

Cholestasis (increased total bilirubin, direct bilirubin, alkaline phosphatase, and y-glutamyl transferase)... [Pg.139]

Liver Total bilirubin, alkaline phosphatase greater than 2 times upper limit of normal01... [Pg.1458]

Liver function, including AST, ALT, alkaline phosphatase, lactate dehydrogenase (LDH), total and conjugated bilirubin a comprehensive metabolic panel can be ordered (i.e., sodium, potassium, chloride, bicarbonate, blood urea nitrogen, creatinine, glucose, calcium, AST, ALT, alkaline phosphatase, albumin, and total bilirubin), but phosphorus, magnesium, and fractionated... [Pg.1508]

Alkaline phosphatase-labeled probes are synthesized so that 18 bases are complementary to sequences on the arms of the bDNA. Three hybridization sites are located on each branch for a total binding capacity of 45 labeled probes per bDNA molecule. The alkaline phosphatase catalyzes the dephosphorylation of chemiluminescent substrate, dioxetane (Lumi-Phos Plus, Lumigen, Detroit, MI). The intensity of the light emission is measured with a plate luminometer as relative luminescent units. [Pg.209]

Alkaline phosphatase, human placental Whole plant, hydroponic Nicotiana tabacum (tobacco) A. tumefaciens transformation of leaf explant Mannopine synthase (mas2 ) N ot reported 20 Jig day"1 g"1 root dry weight (e) 3 % of total medium protein (e) 72... [Pg.18]

Cambella and Antia [385] determined phosphonates in seawater by fractionation of the total phosphorus. The seawater sample was divided into two aliquots. The first was analysed for total phosphorus by the nitrate oxidation method capable of breaking down phosphonates, phosphate esters, nucleotides, and polyphosphates. The second aliquot was added to a suspension of bacterial (Escherichia coli) alkaline phosphatase enzyme, incubated for 2h at 37 °C and subjected to hot acid hydrolysis for 1 h. The resultant hot acid-enzyme sample was assayed for molybdate reactive phosphate which was estimated as the sum of enzyme hydrolysable phosphate and acid hydrolysable... [Pg.424]

Elevations of alkaline phosphatase, /glutamyl transferase, and total bilirubin in patients with cholestatic illness. [Pg.287]

Current nutritional intake Complete blood cell count Serum electrolytes Sodium Potassium Chloride Bicarbonate Magnesium Phosphorous Calcium Serum glucose Serum albumin Markers for organ function Liver function tests Alkaline phosphatase Aspartate aminotransferase Alanine aminotransferase Total bilirubin Prothrombin time or International normalized ratio Renal function tests Blood urea nitrogen Creatinine Fluid balance Input Oral... [Pg.690]

Liver function tests Alkaline phosphatase Aspartate aminotransferase Alanine aminotransferase Total bilirubin Prothrombin time or International normalized ratio (as necessary)... [Pg.690]

Lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, total bilirubin, alkaline phosphatase. [Pg.785]

A few important substances that are routinely analyzed in a clinical laboratory with the aid of an Autoanalyzer are, namely serum-glutamic-oxaloacetic transaminase (SGOT) creatine-phophokinase (CPK) alkaline-phosphatase (AP) belonging to the class of enzymes and a host of biochemical substances, for instance bilirubin, serum albumin, blood urea nitrogen (BUN), uric acid, creatinine, total protein, glucose, cholesterol, besides a few common inorganic ions, such as Cl, Ca2+, K+, Na+. [Pg.66]

Alkaliphilic Bacillus sp. AM-001 was aerobically grown to the early stationary phase at 37°C in alkaline medium (pH 9.0) containing 1% konjak mannan. Total chromosomal DNA obtained by the method of Saito and Miura( 14) was digested with Hindlll restriction enzyme. And 2 to 4 kbp DNA fraction of chromosomal DNA was collected by 1% agarose gel electrophoresis. The plasmid pUC19 was digested with Hindlll and then dephosphorylated with bacterial alkaline phosphatase. After the... [Pg.55]

See other pages where Total alkaline phosphatase is mentioned: [Pg.200]    [Pg.271]    [Pg.176]    [Pg.247]    [Pg.94]    [Pg.200]    [Pg.271]    [Pg.176]    [Pg.247]    [Pg.94]    [Pg.422]    [Pg.210]    [Pg.598]    [Pg.1457]    [Pg.1507]    [Pg.1509]    [Pg.53]    [Pg.173]    [Pg.291]    [Pg.174]    [Pg.593]    [Pg.103]    [Pg.794]    [Pg.172]    [Pg.293]    [Pg.235]    [Pg.85]    [Pg.43]    [Pg.85]    [Pg.354]    [Pg.83]    [Pg.131]   
See also in sourсe #XX -- [ Pg.191 ]



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