Konjak mannan

Alkaliphilic Bacillus sp. AM-001 was aerobically grown to the early stationary phase at 37°C in alkaline medium (pH 9.0) containing 1% konjak mannan. Total chromosomal DNA obtained by the method of Saito and Miura( 14) was digested with Hindlll restriction enzyme. And 2 to 4 kbp DNA fraction of chromosomal DNA was collected by 1% agarose gel electrophoresis. The plasmid pUC19 was digested with Hindlll and then dephosphorylated with bacterial alkaline phosphatase. After the... 

The only commercially important glucomannan is konjak gum also known as konjak mannan. Konjak flour is obtained from the starchy tubers of the plant Amorphophallus konjac (Araceae), grown in subtropical to tropical eastern Asia (Japan, China and Indonesia). 

Konjak mannan dissolves in water to give highly viscous dispersions, which are pseudoplastic systems. Heating with a small... 

Mannans of legume seeds (guar, carob galacto-mannans, konjak mannans) are constituents of native food in India, Japan and Pakistan. - Guar gum or - locust bean gum are widely used as stabilizers... 

Mannans have been found in some kinds of plants such as endosperms of copra and ivory palm nuts, guar beans, locust beans, coffee beans and roots of konjak Amorphophallus konjac). Most of these saccharides are used only in the food and feed processing industries. Recently, it has been reported that manno-oligosaccharides are useful as one of the best growth factors for Bifidobacterium sp. and Lactobacillus... 

E, co/i JMlOl (pMAH3) was grown aerobically in LB broth for 24 h at 37°C. The P-mannanase activity was located mainly in the periplasmic (53%) and intracellular (43%) fractions. No induction of the enzyme could be detected in the presence or absence of mannan, such as konjak, locust bean and copra, in the growth medium. 

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