Total binding

The membrane (or cell) preparation is added to the tubes to begin the binding reaction. The reagents are equilibrated for 30 to 90 minutes (time required for equilibration must be determined experimentally) and then the amount of bound ligand is quantified (either by separation or reading of scintillation proximity beads). The nsb and total binding are obtained from this experiment as shown (in bound pM). 

The total binding and nsb are plotted as a function of added radiolabel (as shown in Figure 12.1a), and fit simultaneously with nonlinear curve fitting... 

The nsb for this example was shown to be 15.2 0.2pM/mg protein. This value is subtracted from the total binding numbers or the total binding fit to displacement curves. Total binding with a representation of nsb is used in this example and is shown in Figure 12.2a. 

Similar calculations can be made for other nuclei. A significant comparison between nuclear binding energies can be made if we divide the total binding energy of each nucleus by the... 

C22-0006. Fluorine has only one stable Isotope, F. Compute the total binding energy and the binding energy per nucleon for this nuclide. 

Figure 2. Influence of the ionic strength and the polymer concentration on the binding isotherms of Pb2+ by sugar-beet pectins in water (empty symbols) and in 0.1 M NaNOs (full symbols) at 25°C ( ) 2 mequiv. COO. l-, ( ) 8 mequiv. COO-.l-i (—) total binding of added Pbz+.

Rodrigues, G.C., Ourdane, M.A., Bieron, J., Indelicato, P. and Lindroth, E. (2001) Relativistic and many-body effects on total binding energies of cesium ions. Physical Review A, 63, 012510-1-012510-10. 

Resin evaluation of both new and used resins (titration of total binding sites, total protein capacity, flow vs. pressure, particle size distribution, total organic carbon removed by cleaning procedures, and microbial and endotoxin analysis)... 

Alkaline phosphatase-labeled probes are synthesized so that 18 bases are complementary to sequences on the arms of the bDNA. Three hybridization sites are located on each branch for a total binding capacity of 45 labeled probes per bDNA molecule. The alkaline phosphatase catalyzes the dephosphorylation of chemiluminescent substrate, dioxetane (Lumi-Phos Plus, Lumigen, Detroit, MI). The intensity of the light emission is measured with a plate luminometer as relative luminescent units. 

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