Stanozolol

Stanozolol, 17P-hydroxy-17a-methyl-5a-androst-2-eno(3,2-c)-pyrazole (Fig. 4.5,17), represents one of the most frequently misused anabolic androgenic steroids with analytical peculiarities due to the presence of 

Sample preparation Condition a 1 mL 100 mg Bond Elut Cl SPE cartridge with 2 column vol of MeCN and 2 column vol of water. Mix 500 (xL serum with 25 xL 5 (xg/mL IS in MeCN, add 500 (xL water, vortex for 5 s, add to the SPE cartridge, wash with 2 column vol of water, wash with 1 column vol of MeCNiwater 10 90, elute with 1 mL MeCN water 45 55, concentrate the eluate to 250 xL xmder a stream of nitrogen at 45°, inject a 50 m-L aliquot. 

Mobile phase MeCN 100 mM pH 2.5 sodium phosphate buffer 15 85 Flow rate 1 Injection volume 50 Detector UV 230 

Lampert, B.L. Stewart, J.T. Determination of anabolic steroids and zeranol in human serum by isocratic reverse phase HPLC on silica, J.Liq.Chromatogr., 1989,12, 3231-3249. 

Chemical Name 17-methyl-2H-5o -androst-2-eno[3,2-c] pyrazol-17/3-oi Common Name Androstanazoie Structural Formula  

To a stirred solution of 1.00 gram of 17/3-hydroxy-17o -methyl-4-androsteno[3,2-c] pyrazole in 200 ml of tetrahydrofuran and 400 ml of liquid ammonia was added 2.12 grams of iithium wire during 5 minutes. The dark biue mixture was stirred for 45 minutes. A solution of 40 ml of tertiary-butyl alcohol in 160 ml of diethyl ether was added with stirring. 

After 15 minutes, 25 ml of ethanol was added with stirring. The mixture turned colorless after several hours, and the liquid ammonia was allowed to evaporate and the mixture was allowed to warm to room temperature over a period of about 15 hours. 

The solvent was evaporated to yield a colorless solid residue, which was taken up In ethyl acetate-ice water. The two layers were separated and the aqueous layer was extracted with ethyl acetate. The combined organic layers were washed with water, saturated sodium chloride solution and filtered through anhydrous sodium sulfate. The solvent was evaporated to yield 1.20 grams of light tan crystals, MP 151° to 155°C, ultraviolet maximum at 224 mp (E = 4,095). Two recrystallizations from ethanol afforded 1st crop, 0.619 grams (62%) of colorless crystals (dried at 120°C in vacuo for 17 hours), (VIP 232.8° to 238.0°C, ultraviolet maximum at 224 mp (E = 4,840) 2nd crop, 0.142 gram (14%) of colorless crystals, MP 234° to 242°C. 

Manson, A.J. U.S. Patent 3,030,358 April 17,1962 assigned to Sterling Drug Inc. 

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The pharmaceutical industry has developed and studied a number of anabolic steroids for use in vet erinary medicine and in rehabilitation from injuries that are accompanied by deterioration of muscles The ideal agent would be one that possessed the an abolic properties of testosterone without its andro genic (masculinizing) effects Methandrostenolone (Dianabol) and stanozolol are among the many syn thetic anabolic steroids that require a prescription... 

One anabohc steroid, the presence of which has proven difficult to analyze, is stanozolol [10418-03-8] C2 H22N20. A metaboUte of the parent dmg, hydroxy stanozolol, detected in equine urine eight hours after ingestion of the parent dmg is actually identified, usually at very low levels. Analysis was done by Ic/ms/ms which had a shortened analysis time advantage over gc/ms procedures because of the elimination of the need for a derivatization step (33). 

The uses of the various anabolic steroids include management of anemia of renal insufficiency, control of metastatic breast cancer in women, and promotion of weight gain in those with weight loss after surgery, trauma, or infections. Stanozolol is used prophylactically... 

C3H9O2 109-94-4) see Danazol Drostanolone Fluorouracil Formeboloue, Metapramine Oxymetholone Perhexiline Stanozolol Tetroxoprim Trilostane Trimethoprim ethyl formylacetate... 

Prophylactic treatment of Cl INH deficiency consists of Cl INH replacement infusions, androgens (danazol, stanozolol, oxymethalone) or antifibrinolytic agents. Acute treatment employs Cl INH replacement (where available), fresh-frozen plasma, and placement of an airway when significant airway obstruction is evident. Abdominal attacks require IV fluid, analgesics, and watchful waiting . 

Fernandez L, Chirino R, Boada LD, Navarro D, Cabrera N, Del Rfo I, Dfaz-Chico BN (1994) Stanozolol and danazol, unlike natural androgens, interact with the low affinity glucocorticoid binding sites from male rat liver microsomes. Endocrinology 134 1401... 

Luzardo OP, Machln RP, Dlaz-Chico BN, Fernandez L (2000) Photoaffinity labeling identification of a specific binding protein for the anabolic steroids stanozolol and danazol an oligomeric protein regulated by age, pituitary hormones, and ethinyl estradiol. Endocrinology 141 3377... 

Stanozolol Kit for stanozolol, ELISA Stanozolol ELISA test kit Tecna In Vitro Biologies Ltd http //www.invitrobiologics.com... 

Androgens Testosterone Boldenone Nandrolone Methyltestosterone Trenbolone Stanozolol Growth promoter in livestock... 

Anabolic Agents—includes anabolic androgenic steroids such as stanozolol and other... 

Figure 8.2. Effect of mass spectral resolution on the detection of a metabolite of stanozolol in a real sample.

A7. Albers, J. J., Taggart, H, M., Applebaum-Bowden, D., Haffner, S., Chesnut, C. H., Ill, and Hazzard, W. R., Reduction of lecithin-cholesterol acyltransferase, apolipoprotein D and the Lp(a) lipoprotein with the anabolic steroid Stanozolole. Biochim. Biophys. Acta 795, 293-296 (1984). 

Anabolics are testosterone derivatives (e.g., clostebol, metenolone, nan-drolone, stanozolol) that are used in debilitated patients, and misused by athletes, because of their protein anabolic effect. They act via stimulation of androgen receptors and, thus, also display androgenic actions (e.g., virilization in females, suppression of spermatogenesis). 

Stanozolol Synthetic androgen Treatment of some clinical presentations of Behfet s syndrome and management of hereditary angioedema... 

Deca-Durabolin), methandrostenolone Dianabot), oxymetholone (Anadrol, Adroyd),stanozolol (Winstrol), and oxandrolone (Anavar). 

B. Finasteride is a 5a-reductase inhibitor, which essentially makes dihydrotestosterone unavailable to the prostate but does not reduce serum testosterone levels. The decreased prostatic levels of dihydrotestosterone frequently result in a size regression of the prostate, while the relatively normal testosterone levels minimize a depressed libido. Flutamide and spironolactone exhibit antiandrogen effects by competing for the androgen receptor ketoconazole inhibits testosterone synthesis and stanozolol is an oral anabolic androgen preparation. 

Contraindications Cardiac impairment, hypercalcemia, prostatic or breast cancer in males, severe liver or renal disease, hypersensitivity to stanozolol or its components... 

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