In vitro biology

Desbrow, C., Routledge, E.J., and Brighty, G.C. et al. (1998). Identification of estrogenic chemicals in STW effluent. 1. Chemical fractionation and in vitro biological screening. Environmental Science and Technology 32, 1549-1558. 

On the basis of these findings, and of recent in vivo and in vitro biological studies [3], the compound LR-B/081 was selected as a candidate for development, and is now undergoing clinical investigation for the treatment of hypertension. 

Schwartz, J.L. 1993. In vitro biological methods for determination of carotenoid activity. Methods Enzymol 214 226-256. 

Trenbolone Trenbolone ELISA test kit In Vitro Biologies Ltd http //www.invitrobiologics.com/... 

Table 6.5. IN VITRO BIOLOGICAL PROPERTIES OF SEVERAL QUINOLONE ANTIBACTERIAL AGENTS [22]...

Tyson, C.A. and Frazier, J.M. (1993). Methods in Toxicology In Vitro Biological Systems. Academic Press, San Diego, CA. 

It was against this background that the research into feverfew in the early 1980 s was performed. A number of investigations have now been carried out into the plant s in vitro biological actions, the chemical components responsible for these actions and suggestions have been put forward as to its mode of action. Furthermore, a limited amount of data are available about the clinical effects of feverfew. The aim of this chapter is to give an overview to date of the facts about the herb feverfew with respect to its chemical, biological and clinical properties. 

The fact that many agents which interrupt the synthesis of pyrimidine nucleotides from orotic acid in animals can also inhibit the growth of experimental neoplasms suggests a search for additional antimetabolites whose locus of action is in this metabolic sequence. Two in vitro biological screening systems were developed for this purpose [202—207]. From a study of systems with oxidative energy sources, 5-bromo-[208—209] (Villa), 5-chloro-[210] (Vlllb) and 5-diazo-orotic acid [211] (IX) were found to inhibit the conversion of orotic acid to the uridine nucleotides by 40—100 per cent [202]. 

Isolated cells, tissues, and organs may be grown in culture in a manner where their natural properties (in vivo) are maintained to some extent. Such in vitro biological systems have been used for many years in studies of mutagenic and genotoxic properties, and in studies of mechanisms. 

Santos AF, Zaltsman AB, Martin RC et al (2008) Angiogenesis an improved in vitro biological system and automated image-based workflow to aid identification and characterization of angiogenesis and angiogenic modulators. ASSAY Drug Devel Technol 6 693-710... 

If UV transparency is an issue for sensitive detection, nonvolatile solvents have been used. These include phosphoric acid,152,53 and TEAP, 24 among others. The latter is preferred as it allows for pH adjustment (2.0-8) and is biocompatible in most in vitro biological systems. It has been proposed that the role of the added triethylamine (to phosphoric acid, formic acid, TFA, and acetic acid) is to cap the unreacted silanols present on the silica gel. This issue has been recently addressed in a short review. 54 The TEAP buffer has been used extensively for the isolation of natural products122 and synthetic peptides. 26 Preparatively, the TEAP buffers were found to be good first solvents to use as they are highly resolutive with a selectivity that is often different from that conferred by 0.1% TFA. Since excess TFA will be eliminated by lyophilization, it is used as the last purification and desalting step. 

Input Heat The amount of heat required to produce the evolution of vapors from a solid or liquid. In Vitro Biological processes occurring (experimentally) in isolation from the whole organism. Interior Structural Fire Fighting The act of fire suppression and rescue inside buildings or enclosed structures where a fire has gone beyond the incipient stage. 

Saia, M., Gu, Z.G., Moens, G. Chouroulinkov, I. (1982) In vivo and in vitro biological effects of the flame retardants tris(2,3-dibromopropyl)phosphate and tris(2-chlorethyl)ortho-phosphate. Eur. J. Cancer din. Oncol., 18, 1337-1344... 

Skoog, F. Miller, C.O. (1957). Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Biological Action of Growth Substances, Symp. Soc. Exptl. Biol. 11, pp. 118-131. Cambridge University Press. 

Williamson G, Barron D, Shimoi K, Terao J. 2005. In vitro biological properties of flavonoid conjugates found in vivo. Free Radic Res 39 457 459. 

III. In vitro biological activity specificity and efficiency Cell culture Demonstrate specific antisense effect by various bioassays such as anti-viral and anti-proliferation effect, induction of apoptosis... 

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