Glucocorticoid binding

Chirino R, Fernandez L, Lopez A, Navarro D, Rivero JF, Dfaz-Chico JC, Dfaz-Chico BN (1991) Thyroid hormones and glucocorticoids act synergistically in the regulation of the low affinity glucocorticoid binding sites in the male rat liver. Endocrinology 129 3118... 

Fernandez L, Chirino R, Boada LD, Navarro D, Cabrera N, Del Rfo I, Dfaz-Chico BN (1994) Stanozolol and danazol, unlike natural androgens, interact with the low affinity glucocorticoid binding sites from male rat liver microsomes. Endocrinology 134 1401... 

Fig. 10. The structure of mouse DNA containing the integrated mouse mammary tumour provirus. The integrated viral DNA can be present in many copies. The provirus contains two long terminal repeat sequences, LTR, only one of which (left) is shown in detail here. Each LTR contains sequences termed U3, R and U5 (for details, see Ref. 67). Interspersed between the LTRs are the genes named gag, pol and env, encoding viral coat proteins, reverse transcriptase and envelope proteins, respectively. The glucocorticoid-binding sequence is represented by the black box, and the transcriptional initiation area is indicated by the hatched box.

Folate binding protein has been prepared from goat s milk by adsorption to a methotrexate-Sepharose column [94]. The folate binding protein was then coupled to cyanogen bromide activated Sepharose and used in the purification of folates and for the removal of endogenous folates from reaction mixtures. Cortisol hemisuccinate coupled to an amino Sepharose has been used to purify glucocorticoid binding protein(s) from rat liver... 

Roth, G. S., Age-related changes in specific glucocorticoid binding by steroid-responsive tissues of rats, Endocrinology, 94, 82, 1974. 

C. Effects See Chapter 39 for details. Glucocorticoids bind to intracellular receptors and activate glucocorticoid response elements (GREs) in the nucleus, resulting in synthesis of substances that prevent the full expression of inflammation and allergy. Reduced activity of phospholipase A is thought to be particularly important in asthma because the leukotrienes that result from eicosanoid synthesis are extremely potent bronchoconstrictors and may also participate in the late inflammatory response (Figure 20-4). 

Baxter, J., and G. M. Tomkins. 1970. The relationship between glucocorticoid binding and tyrosine aminotransferase induction in hepatoma tissue culture cells. Proc. Nat. Acad. Sci. U.S.A., 65 709. 

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