Rats, Sprague-Dawley

Parker LA, Gillies T THC-induced place and taste aversions in Lewis and Sprague-Dawley rats. Behav Neurosci 109 71-78, 1995 Pope HG, Yurgelun-Todd D The residual cognitive effects of heavy marijuana use in college students. JAMA 275 521-327, 1996 Robson P Therapeutic aspects of cannabis and cannabinoids. BrJ Psychiatry 178 107-115, 2001... 

Cannon Laboratories (1979) Acute oral toxicity of 1185-13 (dioctyltin dichloride), 28G082, 28H047, 1185-114, 1219-21 (dimethyltin dichloride), 1185-150, 1185-119 and 1185-138 in weanling Sprague-Dawley rats. Reading, PA, Cannon Laboratories Inc., 21 March. 

Pope et al. (1991) found that 7-day-old Sprague-Dawley rat pups were approximately twice as sensitive as 80-100-day-old adults to single subcutaneous doses of methyl parathion the highest nonlethal dose 7.8 mg/kg for the neonates and 18.0 mg/kg for adults. Initially, both neonates and adults exhibited similarly reduced brain acetylcholinesterase activity levels (approximately 10% that of controls) ... 

In vivo biocompatibility was assessed through subcutaneous implantation in Sprague-Dawley rats. PLGA was used as a control polymer. PGS and PLGA implants with the same surface area/volume ratio were implanted in dorsal subcutaneous pockets. A fibrous capsule around PGS (45 pm thick after 35 days implantation) appeared later than that around PLGA (140 pm thick after 14 days implantation). After 60 days of implantation, the implant was completely absorbed with no signs of granulation or scar formation. ... 

The carcinogenicity of technical endosulfan was reevaluated in Sprague-Dawley rats using lower doses of endosulfan (Hoechst 1989a). Endosulfan was administered in the diet for 2 years, and no effect on survival was observed in either sex at any dose. Under the conditions of this assay, dietary consumption of doses as high as 3.8 mg/kg/day by females or 2.9 mg/kg/day by males did not result in an increase in the incidence of any neoplastic lesions in these animals. The results from the Hoechst (1989a) bioassay... 

Three animal studies were located regarding distribution of endosulfan in animals following dermal exposure (Dikshith et al. 1988 Hoechst 1986 Nicholson and Cooper 1977). Endosulfan was detected in the brain (0.73 ppm), liver (3.78 ppm), and rumen contents (0.10 ppm) of calves that died after dermal exposure to a dust formulation of endosulfan (Nicholson and Cooper 1977). Following a single dermal application of aqueous suspensions of 0.1, 0.83, and 10.13 mg/kg C-endosulfan to male Sprague-Dawley rats, low concentrations of endosulfan (ng/g levels) appeared in the blood and tissues (other than skin at and around the application site) after 1 hour (Hoechst 1986). The concentrations of endosulfan in the blood and tissues increased with the time of exposure and were proportional to the dose applied. The liver and kidney appeared to sequester radiolabel relative to the concentrations of radiolabel in the blood or fat. Endosulfan levels were approximately 10 times higher in the liver and kidney than in the fat, blood, and brain throughout the study (Hoechst 1986). 

Animals. Male Spartan strain Sprague Dawley rats weighing 165-210 grams were used. The rats were acclimated to the environment of the metabolism cages five days before dosage. Food and water were provided ad libitum throughout the experiment. 

In male Sprague-Dawley rats, there was a dose-related increase in testicular Leydig cell tumors and a slight increase in tubular renal adenocarcinoma at the 600-ppm exposure level after exposure for 104 weeks (Maltoni et al. 1986, 1988). EPA and other groups regard such increases as indicative of a hazard potential unless there are reasons to rule this out. However, other authorities believe testicular tumors are common in rats that are not exposed to toxic substances. 

The incidence of pulmonary adenocarcinomas was significantly increased over controls in female ICR mice exposed to 150 or 450 ppm reagent grade trichloroethylene for 104 weeks, 5 days/week, 7 hours/day (Fukuda et al. 1983). There was no significant increase in other tumors in the mice or in similarly exposed female Sprague-Dawley rats. The amount of epichlorohydrin (approximately 0.019%) was extremely low in this... 

Maltoni C, Lefemine G, Cotti G, et al. 1988. Long-term carcinogenicity bioassays on trichloroethylene administered by inhalation to Sprague-Dawley rats and Swiss and B6C3F, mice. Arm NY Acad Sci... 

CHANG H 0, CHURCHWELL M I, DELCLOS K B, NEWBOLD, DOERGE D R (2000) Mass spectrometric determination of genistein tissue distribution in diet-exposed Sprague-Dawley rats. J Nutr. 130 1963-70. 

Next, four male Sprague-Dawley rats were administered NDPA-[2,3- H] by intraperitoneal injection (12). After 12 hours, the animals were sacrificed, and RNA and DNA were isolated from the combined livers by standard procedures. Following addition of unlabeled, authentic 7-propylguanine and 7-isopropylguanine as markers, the nucleic acids were hydrolyzed in perchloric acid at... 

We have so far been unable to find an appropriate set of data to adequately test this model, A small set of nitrosamines has been studied by Lijinsky and coworkers in both Fischer and Sprague-Dawley rats (28) although there is considerable scatter the results are consistent with this model. 

Animal treatment. Male 100-g MRC-Wistar or Sprague-Dawley rats were fasted from the evening of the previous day but were given water. Non-fasted 30-35 g Swiss mice were used. Except where specified otherwise, the animals received a standard commercial diet (Wayne Lab Blox, Allied Mills). In the standard experiments, animals were gavaged with 25 mg morpholine (Fisher Scientific Co.) in 10 mL water/rat or 2 mg morpholine in 0.2 mL water/mouse. A stream of NO2 was prepared by suitable mixing (using Teflon tubing) of gas from a cylinder of 106 ppm N0 in N (Linde Division, Union Carbide Corp.) and compressed air. 

In initial ICC studies, animals were treated with MDA or MDMA using the protocol described by Ricaurte et al. (1985). Adult Sprague-Dawley rats (150 to 200 g) reeeived subcutaneous injections of racemic MDA or MDMA every 12 hours for 4 days. Each dose was equivalent to 20 mg/kg of the free base. The rats were sacrificed by intracardiac aldehyde perfusion 2 weeks after the final dose. In order to study subacute effects for evidence of degeneration, additional rats received MDA every 12 hours for 2 days and were sacrificed 24 hours after the last injection. Additional experimental details are described elsewhere (O Heam et al. 1986 O Heam et al. 1988). A series of animals treated identically and in parallel were analyzed for changes in 5-HT levels and density of uptake sites using paroxetine binding (Yeh et al. 1986 Battaglia et al. 1987). 

The pharmacokinetic profile of (16) and its two analogues were investigated in Sprague-Dawley rats. Removal of the metabolically labile tert-butyl group on the aryl moiety slowed metabolism and the rate of clearance. However, the overall half-life of (17a) was unaffected because of a lower volume of distribution. On the other hand, (17b) showed an increased half-life (ca. 3h versus 1 h) compared to (16) and (17a). While the oral bioavailability of (16) was negligible, (17a) and (17b) were better absorbed, with bioavailability values of 39% and 17%, respectively. While undoubtedly improved in terms of pharmacokinetics compared to (16), the bioactivity of (17a) and (17b) awaits validation in vivo. 

Male, Sprague-Dawley rats were implanted under pentobarbital anesthesia with a bipolar electrode in the right dorsal hippocampus for hippocampal stimulation and recording. Stainless steel screws were also placed over the contralateral frontal and parietal cortices for recording cortical seizure activity. The electrodes... 

Throughout these studies, adult female Sprague-Dawley rats weighing about 250 gm were used. During experimentation, they were housed in individual plastic cages. Purina Rat Chow and water were available a d 1 i hi turn. and they were maintained under an automated light cycle with illumination from 6 a.m. to 10 p.m. daily. 

Adult, male, Sprague-Dawley rats obtained from Charles River Labs and weighing 200-250 gm on arrival were used. Throughout the study, the rats were housed individually, with water freely available. Purina Rat Chow was fed after each experimental session and on weekends, in quantities adjusted to maintain the rats between 270 and 350 gm throughout the experiment. 

Angiotensin II. Male Sprague-Dawley rats (300-400 g) were administered either free angiotensin II (A-H) (Sigma product A9525, human synthetic A-H, Asp-Arg-Val-Tyr-Ile-His-Pro-Phe) in 0.9% NaCl solution by subcutaneous injection, or A-H impregnated resin by gastric intubation. The resin was suspended in distilled water such that the rat received no more than a 0.5 mL total suspension volume. The... 

Healy CE, Beyrouty PC, Broxup BR. 1995. Acute and subchronic neurotoxicity studies with tri-n-butyl phosphate in adult Sprague-Dawley rats. Am Ind Hyg Assoc, 56 349-355. 

Healy CE, Nair RS, Lemen JK, et al. 1991. Subchronic and reproduction studies with dibutyl phenyl phosphate in Sprague-Dawley rats. Fundam Appl Toxicol 16 117-127. 

Laham S, Long G, Broxup B. 1984. Subacute oral toxicity of Tri-n-butyl Phosphate in the Sprague-Dawley rat. J Appl Toxicol 4(3) 150-154. 

Monsanto. 1987a. Three-month study of Skydrol 500B-4 administered to male and female Sprague-Dawley rats by inhalation. Study No. 84049. Project No. ML-84-226. 

RTI. 1992. Research Triangle Institute. Two-generation reproductive toxicity study of tributyl phosphate administered in the feed to CD (Sprague-Dawley) rats. RTI Project No. 60C-4652. 

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