Sex linked recessive lethal assay

TK or HPRT forward mutation assays in cultured mammalian ceils Drosophila sex-linked recessive lethal assay... 

Dichloromethane induced DNA single-strand breaks in mammalian cell cultures, but inconclusive or negative effects were reported for induction of gene mutations. It did not induce unscheduled DNA synthesis either in vivo in rodents or in human fibroblast cultures. It was genotoxic in fungi but not in Drosophila in the sex-linked recessive lethal assay. 

Wurgler, F.E. Graf, U. (1981) Mutagenic activity of 10 coded compounds in the Drosophila sex-linked recessive lethal assay. In de Serres, F.J. Ashby, J., eds. Evaluation of Short-Term Tests for Carcinogens. Report of the International Collaborative Program (Progress in Mutation Research, Vol. 1), Amsterdam, Elsevier, pp. 666-672... 

The Working Group was also aware of inhalation studies with dimethylformamide conducted for the United States National Institute of Occupational Health. These involved exposure to 400 ppm [1200 mg/m ] for 7 h in a rat bone-marrow cell cytogenetic study, for 7 h per day for five days in a rat bone-marrow cell cytogenetic study, a male rat dominant lethal assay and a mouse sperm morphology assay and for 2.25 h in a Drosophila melanogaster sex-linked recessive lethal assay. All results were negative. 

MIC was nonmutagenic in the Ames (Salmonella), Drosophila sex-linked recessive lethal assays. MIC induced chromosomal aberrations in cultured Chinese hamster ovary cells. 

SRI. 1985. Stanford Research Institute. Drosophila sex-linked recessive lethal assay of... 

Tripathy NK, Dey L, Majhi B, et al. 1987. Genotoxicity of metacid established through the somatic and germ line mosaic assays and the sex-linked recessive lethal test in drosophila. Arch Toxicol 61 53-57. 

IGE was mutagenic in bacterial assays with and without metabolic activation and in the Drosophila sex-linked recessive lethal (SERE)... 

Bacillus subtilis H17 rec MA5 recT, DNA damage assay Saccharomyces cerevisiae JDl, gene conversion Drosophila melanogaster, sex-linked recessive lethal mutation Unscheduled DNA synthesis, primary rat hepatocytes in vitro Sister chromatid exchange, Chinese hamster ovary CHO cells in vitro... 

Glycidol gave a positive response in assays of prophage induction and SOS repair m Escherichia coli. Results were uniformly positive in several Salmonella typhimurium reverse mutation assays and in two fungal mutation assays. The sex-linked recessive lethal mutation assay and the heritable translocation test in Drosophila melanogaster also gave positive results. 

Genotoxic Effects. No studies were located regarding the genotoxic effects of acrolein in humans or animals by inhalation, oral, or dermal routes. Acrolein was not mutagenic in vivo as judged by the dominant lethal assay in the mouse (Epstein et al. 1972) or the sex-linked recessive lethal test in... 

When tested in the Salmonella assay, acrolein was weakly positive. It was not mutagenic in the dominant lethal assay in the mouse and in the Drosophila sex-linked recessive lethal test, and negative for chromosome aberrations when tested in cultured Chinese hamster ovary cells however, there was an increase in the frequency of sister-chromatid exchanges. 

Dimethyl ether is inactive in genetic tests including the Salmonella assay (with and without metabolic activation in at least four strains), HPRT reversion in CHO cells, DNA repair/synthesis in rat liver cells, and the sex-linked recessive lethal test in the fruit fly. 

TBHP did not produce a genotoxic response in the cell transformation assay, but did produce a mutagenic response in the Ames Salmonella) and mouse lymphoma mutagenesis assays. TBHP was tested for the induction of sex-linked recessive lethal mutations in Drosophila melanogaster, and was positive at a dose of 2000 ppm when administered to males by feeding. 

Mixed results were observed for in vitro tests of pyrene. Negative results were obtained for pyrene in DNA damage assay in Escherichia coli and Bacillus subtilis. Both positive and negative results were observed in bacterial gene mutation test. Pyrene did not induce an increase in sex-linked recessive lethal gene in Drosophila. It increased the incidence of mitotic gene conversion but not other genetic endpoint in yeast. 

The pesticides chIorpyrifos, ,4-D acid, 2,4-DP acid, dicamba, dinoseb, and propaniI were positive in the bacterial relative toxicity assays (propaniI in B. subtiI is only), but in all other assays, produced no activity (Table IV). Increased mitotic recombination was induced by azinphos-methyI, crotoxyphos, cacodylic acid, and parathion-methyl in cerevisiae D3 disulfoton enhanced UDS in WI-38 cells. As shown in Table V, however, these pesticides produced no other effects. Bromacil and simazine were positive only in the Drosophila sex-linked recessive lethal test (Table VI). 

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