Cultured human fibroblasts

Protease nexin (From cultured human fibroblasts) [148263-58-5], Purified by affinity binding of protease nexin to dextran sulfate-Sepharose. [Farrell et al. Biochem J 237 707 1986.]... 

Chojkier, M., Houghim, K., Solis-Hemizo, J. and Brenner, D.A. (1989). Stimulation of collagen gene expression by ascorbic acid in cultured human fibroblasts A role for lipid peroxidation J. Biol. Chem. 264, 16957-16962. 

Pagano, M., Pepperkok, R., Lukas, J., Baldin, V., Ansorge, W Bartek, J., and Draetta, G. (1993). Regulation of the cell cycle by the cdk2 protein kinase in cultured human fibroblasts. J. Cell Biol. 121 101-111. 

Wester K, Andersson A, Ranefall P, et al. Cultured human fibroblasts in agarose gel as a multi-functional control for immunohistochemistry. Standardisation of Ki67 (MIB1) assessment in routinely processed urinary bladder carcinoma tissue. J. Pathol. 2000 190 503-511. 

Protonated N-hydroxy arylamines have also been proposed to be ultimate carcinogens for the urinary bladder (16,17,140,141) since urine pH is slightly acidic in a number of species (14,142). Furthermore, pharmacokinetic studies have shown that increased urine acidity and decreased frequency of urination are predictive of relative species susceptibility to urinary bladder carcinogenesis (142) and neoplastic transformation of cultured human fibroblasts by N-hydroxy arylamines is greatly enhanced by incubation at pH 5 as compared to pH 7 (143). 

LOX-dependent superoxide production was also registered under ex vivo conditions [55]. It has been shown that the intravenous administration of lipopolysaccharide to rats stimulated superoxide production by alveolar and peritoneal macrophages. O Donnell and Azzi [56] proposed that a relatively high rate of superoxide production by cultured human fibroblasts in the presence of NADH was relevant to 15-LOX-catalyzed oxidation of unsaturated acids and was independent of NADPH oxidase, prostaglandin H synthase, xanthine oxidase, and cytochrome P-450 activation or mitochondrial respiration. LOX might also be involved in the superoxide production by epidermal growth factor-stimulated pheochromo-cytoma cells [57]. 

In an in vitro assay with cultured human fibroblasts, aniline produced only marginal increases in sister chromatid exchanges at the highest dose tested, 10 mM whereas two metabolites of aniline, 2-aminophenol and jV-phenylhydroxylamine, doubled the frequency of sister chromatid exchanges at the highest tested nontoxic concentration, 0.1 mM (Wilmer et al. 1981). 

There is no conclusive evidence from studies of cancers in dye workers that aniline is the causative agent. Two known metabolites of aniline induced sister chromatid exchange in the single study with cultured human fibroblasts. No studies on possible reproductive or developmental effects in humans associated with aniline exposures were located. 

Oya-Ohta, Y., Kaise, T., and Ochi, T., Induction of chromosomal aberrations in cultured human fibroblasts by inorganic and organic arsenic compounds and the different roles of glutathione in such induction, Mutat. Res., 357, 123, 1996. 

MacKenzie, K. L., S. Franco, C. May, M. Sadelain, and M. A. Moore. 2000. Mass cultured human fibroblasts overexpressing hTERT encounter a growth crisis following an extended period of proliferation. Exp Cell Res 259(2) 336-50. 

Brunk, U.T., Dalen, H., Roberg, K.., and Hellquist, H.B., 1997, Photo-oxidative disruption of lysosomal membranes causes apoptosis of cultured human fibroblasts. Free Radio. 

Sodium borate tested negatively in the Ames bioassay but was found to be cytotoxic to cultured human fibroblasts."... 

Resorcinol was not genotoxic in bacterial assays or in in vivo mammalian assays it did cause chromosomal aberrations in human lym-phoqn es in vitro but not in cultured human fibroblasts. ... 

Mutagenesis Aspirin induced chromosome aberrations in cultured human fibroblasts. 

Although camosine s ability to suppress both formation and reactivity of some of the age-associated macromolecular modifications (e.g., protein-protein cross-links and protein AGEs) could contribute to its apparent antisenescent effects, these prophylactic actions cannot by themselves explain the dipeptide s apparent rejuvenating activity towards cultured human fibroblasts observed by McFarland and Holliday (1994,... 

Carnosine can affect gene expression. Ikeda et al. (1999) showed that carnosine markedly upregulates vimentin synthesis in cultured rat fibroblasts, while an association between carnosine and vimentin, a cytoskele-tal, intermediate filament protein has been noted in glial cells and neurons (Bonfanti et al., 1999). Interestingly, it has also been shown that the protease, oxidized protein hydrolase (OPH), is coexpressed with vimentin in COS cells (Shimizu et al., 2004). Thus, it is at least possible that carnosine could induce synthesis of OPH in the cultured human fibroblasts and thereby increase the cellular ability to eliminate oxidized... 

Cultured human fibroblasts Kligerman 1984 Sister chromatid exchange No data - Cheng and... 

Menendez, R., S. I. Fernandez, A. Del Rio, et al. Policosanol inhibits cholesterol biosynthesis and enhances low density lipoprotein processing in cultured human fibroblasts. Biol Res... 

Snyder, R.D. Matheson, D.W. (1985) Nick translation—a new assay for monitoring DNA damage and repair in cultured human fibroblasts. Environ. Mutag., 1, 267-279... 

These interrelationships may occur in the plasma compartment, on the surface of cells, or within the cell. Our purpose here will be to review briefly some of the previous work in the above area and to present some of our recent, preliminary data on GSL and lipoprotein metabolism. Our approach has been to study simultaneously cultured human fibroblasts derived from both normal subjects and those heterozygous or homozygous for familial hypercholesterolemia (FH), a relatively common disorder of cholesterol and low density lipoprotein (LDL) metabolism. 

The plasma lipoproteins contain eight major apoproteins, the structure and function of which have recently been reviewed (5). Briefly, the primary amino acid sequence is known for five of these apoproteins. ApoB, a highly hydrophobic protein, is found in chylomicrons, VLDL and LDL. It is the major polypeptide in LDL and has been shown to be responsible, in part, for the recognition of LDL by its receptor in cultured human fibroblasts (7,10). The major polypeptides of HDL are apoA-I and apoA-II apoA-l activates lecithin cholesterol acyl transferase. In addition, studies on the cellular level suggest that apoA-I may regulate the content of the lipids in the cell membrane (8). 

The metabolism of HDL probably involves interaction with both hepatic and peripheral cells, as well as with other lipoproteins. HDL may remove cholesterol from tissues, the "scavenger hypothesis (11,12). The cholesterol may then be esterifed by the action of lecithin cholesterol acyl transferase. HDL may provide cholesterol to the liver for bile acid synthesis (13) and some HDL may be catabolized by the liver in the process. HDL has not been found to interfere with the binding of LDL in cultured human fibroblasts (6). However, in cultured human arterial cells, porcine or rat hepatocytes, and rat adrenal gland, there appears to be some competition of HDL with LDL binding sites, suggesting the presence of a "lipoprotein-binding" site (14). 

The metabolism of GSLs has been studied in cultured human fibroblasts from normal subjects, patients with lipid storage diseases, and those with FH. The content of the GSLs, as well as activities of the biosynthetic enzymes, the glycosyltransferases and the lysosomal GSL hydrolases,have been studied. Complex gangliosides, such as M1, GDla, have been found in this cell system to serve as receptors for cholera toxin and thyrotropin, respectively (24-26). More recently, GT1 and GDla have been postulated to be receptors for fibronectin in cultured fibroblasts... 

Wright, J.F., Kurosky, A., Pryzdial, E.L., and S. Wasi, 1995, Host cellular annexin II is associated with cytomegalovirus particles isolated from cultured human fibroblasts. J Virol. 69(8) 4784-91. 

Schachtschabel, D.O. and Wever, J., Age-related decline in the synthesis of glycosaminoglycans by cultured human fibroblasts, Mech. Aging Dev., 8, 257, 1978. 

Role of amino add transport system A in the control of cell volume in cultured human fibroblasts. Cell. Physiol. Biochem. 1,131-142. 

G2. Gianturco, S. H., Gotto, A. M., Jr., Jackson, R. L., Patsch, J. R., Sybers, H. D., Taunton, O. D., Yeshurun, D. L., and Smith, L. C., Control of 3-hydroxy-3-meth-ylglutaryl-CoA reductase activity in cultured human fibroblasts by very low density lipoproteins of subjects with hypertriglyceridemia. J. Clin. Invest. 61, 320-328 (1978). 

Innerarity, T. L., and Mahley, R. W., Enhanced binding by cultured human fibroblasts of apo-E-containing lipoproteins as compared with low density lipoproteins. Biochemistry 17, 1440-1447 (1978). 

Soutar, A. K., Night, B. L., and Myant, N. B., The characterization oflipoproteins in the high density fraction obtained from patients with familial lecithin cholesterol acyltransferase deficiency and their interaction with cultured human fibroblasts. /. lipid Res. 23, 380-390 (1982). 

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