Radioimmunoassay studies

Dunnette J, Weinshilbourn RM (1977) Inheritance of low immunoreactive human plasma dopamine hydroxylase. Radioimmunoassay studies. J Clin Invest 609 1080... 

BERNSTEIN, D.I., PATTERSON, R. ZEISS, C.R. (1982) Clinical and immunological evaluation of trimellitic anhydride- and phthalic anhydride-exposed workers using a questionnaire and comparative analysis of enzyme-linked immunosorbent and radioimmunoassay studies. Journal of Allergy and Clinical Immunology, 69, 311-318. 

B19. Beckett, G. J., Howie, A. F., Hume, R., Matheroo, B., Hiley, C., Jones, P., and Strange, R. C., Human glutathione S-transferases Radioimmunoassay studies on the expression of alpha, mu, and pi class isoenzymes in developing lung and kidney. Biochem. Biophys. Res. Common. 1036, 176-182 (1990). 

P17. Postmes, T. J., van Hout, J. C., Saat, G., Willems, P., and Coenegracht, J., A radioimmunoassay study and comparison of seasonal variation in plasma triiodothyronine and thyroxine concentrations in normal healthy persons. Clin. Chim. Acta 50, 189-195 (1974). 

Relatively stable proteins isolated from human heart and from human and rhesus monkey skeletal muscles, are capable of binding to /3-D-galactosides. Gel precipitation and radioimmunoassay studies with rabbit antiserum to calf heart lectin revealed antigenic cross-reactions between the primate and bovine muscle lectins. The lectin from calf heart shows a greater specificity towards the terminal disaccharide (lactosamine) units of blood-group antigen precursor chains than does the Ricinus communis lectin. 

Immunoglobulins G from antisera specific for human chorionic gonadotrophin, carcinoembryonic antigen, a-foetoprotein, and casein have been isolated by affinity chromatography on columns of immobilized staphylococcal protein A, before being themselves immobilized on supports.The resulting coupled antibodies provide a greater sensitivity and convenience in radioimmunoassay studies than the conventional double antibody-precipitation methods. 

Medroxyprog esteroneAcetate. Accurate pharmacokinetic and metaboHsm studies on MPA have been difficult because the radioimmunoassays employed caimot differentiate between MPA and its metaboHtes (346). Comparison of MPA plasma levels assayed by hplc and radioimmunoassay show that radioimmunoassay may overestimate intact MPA concentrations by about fivefold (347). However, values of the mean elimination half-life of MPA were similar, being 33.8 and 39.7 h when measured by hplc and radioimmunoassay, respectively (347). Approximately 94% of MPA in the blood is bound to albumin (348). When taken orally, MPA is rapidly absorbed with Htde or no first-pass metaboHsm (13). Peak semm levels ate reached after 3 h. Steady state occurs after three days of daily adininistration (349). The pharmacokinetics of MPA when adininistered in a depot formulation have been described (350). 

Mertes PM, Moneret-Vautrin DA Skin reactions to intradermal neuromuscular blocking agent injections a randomized multicenter trial in healthy volunteers. Anesthesiology 2007 107 245. Moneret-Vautrin DA, Gueant JL, Kamel L, Laxenaire MC, el Kholty S, Nicolas JP Anaphylaxis to muscle relaxants cross-sensitivity studied by radioimmunoassays compared to intradermal tests in 34 cases. J Allergy Clin Immunol 1988 82 745. 

Methods of detection, metabolism, and pathophysiology of the brevetoxins, PbTx-2 and PbTx-3, are summarized. Infrared spectroscopy and innovative chromatographic techniques were examined as methods for detection and structural analysis. Toxicokinetic and metabolic studies for in vivo and in vitro systems demonstrated hepatic metabolism and biliary excretion. An in vivo model of brevetoxin intoxication was developed in conscious tethered rats. Intravenous administration of toxin resulted in a precipitous decrease in body temperature and respiratory rate, as well as signs suggesting central nervous system involvement. A polyclonal antiserum against the brevetoxin polyether backbone was prepared a radioimmunoassay was developed with a sub-nanogram detection limit. This antiserum, when administered prophylactically, protected rats against the toxic effects of brevetoxin. 

S.M. Hsu, L. Raine, and H. Fanger, A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am. J. Clin. Pathol 75, 734-738 (1981). 

Henderson SR, Baker C, Fink G (1977) Oestradiol-17beta and pituitary responsiveness to luteinizing hormone releasing factor in the rat a study using rectangular pulses of oestradiol-17 beta monitored by non-chromatographic radioimmunoassay. J Endocrinol 73 441-453... 

Study of chemical pathways in method development. Isotope dilution methods. Radioimmunoassay very important in biochemistry and medicine. Neutron activation analysis used for trace elements in geo-chemistry, semiconductor technology, pollution studies and forensic science. Relative precision of counting 1% if 104 counts are recorded. Assessment of pollution by radionuclides. 

The methodology of the radioimmunoassay have been studied extensively and outlined in a sequential manner as follows ... 

Radioactive isotopes provide a very convenient way of monitoring the fate or metabolism of compounds that contain the isotopes. When used in this way, the isotope is described as a tracer and compounds into which the radioactive atom has been introduced are said to be labelled or tagged. The labelled molecules need only comprise a very small proportion of the total amount of the unlabelled radioactive substance because they act in the same way as the non-radioactive substance but can be detected very much more easily. The varied applications of tracers in biochemistry range from studies of metabolism in whole animals or isolated organs to sensitive quantitative analytical techniques, such as radioimmunoassay. Phosphorus-32 is used in work with nucleic acids, particularly in DNA sequencing and hybridization techniques. In these instances the isotope is used as a means of visualizing DNA separations by autoradiographic techniques. 

Numerous testing systems and protocols have been used to study 5-LO inhibitors in different laboratories, complicating attempts to compare compounds and series. In vitro, a variety of both cell-free and cellular preparations have been employed as primary screens. The most commonly used cell-free system is the crude cytosolic fraction from broken RBL-1 cells [25] various broken neutrophil preparations are also used, and more recently purified enzymes have occasionally been employed. The formation of 5-LO products is generally determined by radioimmunoassay or (in older work) HPLC or bioassay methods. 

Clinical pharmacokinetic investigations with both vinblastine and vincristine have revealed a triexponential elimination pattern. As for preclinical pharmacokinetic studies, early information was obtained by analysis of samples from patients receiving radiolabeled drug, but more recent investigations make use of radioimmunoassays. It should be noted that radioimmunoassays, while very sensitive in terms of detecting drugs, may also measure structurally related drug metabolites, and some caution is needed in interpretation of pharmacokinetic results obtained from such studies. 

Luft, R. Yallow, R. S. "Radioimmunoassay Methodology and Applications in Physiology and in Clinical Studies" George Thieme Verlag Stuttgard, 1974. 

L/mole). It cross-reacts at >90% with saxitoxin but at <1% with neosaxitoxin. This antibody, when used in an anti-rabbit IgG "second antibody" radioimmunoassay format, can detect pmole quantities of saxitoxin. This assay has been shown to be a simple and efficient method for the analysis of saxitoxin in clam extracts. The lack of antibody cross-reactivity to the neosaxitoxin sub-group of the paralytic shellfish poisons limits the general utility of the assay to neurophysiology studies and to certain clam species which preferentially accumulate saxitoxin. However, the radioimmunoassay serves as a good precursor in the development of an enzyme immunoassay for the paralytic shellfish poisons. 

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