Organic isolation

An examination of stored industrial water supplies showed that 98% of the contaminants were Gram-ne live bacteria other organisms isolated were MrcrococcMv spp., Cytophaga spp., yeast, yeast-like fungi and actinomycetes. 

Figure 10. Tissue specific expression of p-subunit proteins in floral tissues. Stage 3 (fully opened) flowers were collected, dissected and cell wall proteins (5 pgm) from the indicated organs isolated and analyzed for p-subunit antigen. Note the high level of expression in stigma/style and anthers/pollen and restriction of the larger antigen to stigma/style tissues. PGl lane, 1 gg of purified fruit PGl protein.

Pharmacologic management of infections should cover the gram-positive organisms that most frequently cause access-related infections. Patients who have positive blood cultures should receive treatment tailored to the organism isolated. Preventive measures for access-related infections include mupirocin at the exit site and povidone-iodine ointment. The recommendations of the NKF for treatment of infections associated with hemodialysis are listed in Table 23-9. 

Blood cultures are only positive about 4% of the time but should be obtained for complicated cases. Cultures aspirated from the lesion have an organism isolation rate of less than 20% but also may be considered. 

Cirrhosis Peritoneal Cefotaxime Regimen based on organism isolated 1. Add clindamycin or metronidazole if anaerobes are suspected 2. Other third-generation cephalosporins, extended-spectrum penicillins, aztreonam, and imipenem as alternatives 3. Aminoglycoside with antipseudomonal penicillin... 

Actinomycetes are typically most abundant in well drained, circumneu-tral to alkaline soils having abundant organic matter. Water-logging and low pH may reduce populations (37, 38). The numbers of actinomy-cetous organisms isolated from the various soil samples in our study follow this pattern. No clear trend emerged as to a particular edaphic or biotic factor causing an increase in the proportion of inhibitory isolates in a soil sample. 

Work on the fermentation of microbial polysaccharides started in the mid 1970 s, with the aim of producing improved polymers. Many thousands of samples were screened for microorganisms which produced viscous polymers. Out of over 2000 such slime producing organisms isolated, only one, identified as a Pseudomonas species, now NCIB 11592, seemed to produce a polymer with interesting new properties. 

In approximately 50% of patients with infectious diarrhea enrolled in clinical trials the most common organism isolated and presumed causative was E. coli. Treatment with the antibiotic led to clearance of the bacterium in... 

CBP is most commonly caused by E. coli, with other gram-negative organisms isolated much less often. 

Quensen et al. [69] showed that micro-organisms isolated from Hudson river sediment dechlorinated most polychlorinated biphenyls in Aroclor 1242 under anaerobic conditions in the laboratory. The higher the polychlorobiphenyl concentration, the more rapid the rate of dechlorination. The possible mechanisms involved are discussed. The products of dechlorination were less toxic than the original compounds and were more readily degraded by aerobic bacteria wastewater containing... 

Comparison of evolved 14CO from four sterile and non-sterile soils 60 days after treatment with MSMA-l2tC showed that from 1.7 to 10.0% of the MSMA-14C was degraded in non-sterile soil, as compared with 0.7% in steam-sterilized controls. Four soil micro-organisms isolated in pure culture degraded... 

Kameyama T, Takahashi A, Kurasawa S, Ishizuka M, Okami Y, Takeuchi T, Umezawa H (1987) Bisucaberin, a New Siderophore, Sensitizing Tumor Cells to Macrophage-mediated Cytolysis. I. Taxonomy of the Producing Organism, Isolation and Biological Properties. J Antibiotics 40 1664... 

Which of these metaphorical roles does the suprachiasmatic nucleus of the hypothalamus (SCN) play as it influences rhythms in the rest of the body The answer hinges upon the interpretation of the effects of SCN lesions and on the rhythmic behaviour of tissues and organs isolated from SCN influences. 

In severe bacterial infections that are difficult to eradicate, such as endocarditis or osteomyehtis, it may be important to ensure that the patient s serum remains bactericidal at the lowest, or trough, concentration in the dosing interval. Dilutions of patient s serum can be incubated with the organism isolated from the patient and the minimum bactericidal concentration determined through serial dilutions. Treatment is considered adequate if the serum remains bactericidal at a dilution of 1 8. 

Figure 1. Schematic of the strategy for the coupled bioassay-analytical fractionation of residue organics isolated from aqueous environmental samples.

Table I. Mutagenic Activity for Residue Organics Isolated from a Variety of Aqueous Environmental Samples...

Figure 2. Absorbance (254 nm) profile of the analytical-scale HPLC separation of 25 pg of residue organics isolated from an industrially impacted influent waste water. The separations were accomplished via reverse-phase HPLC (top) and normal-phase HPLC (bottom). Mobile phases used in these separations were water (fyO), acetonitrile (CH3CN)> methylene chloride (MECL), hexane (HX), and isopropyl alcohol (IPROH), as indicated.

Figure 3. Absorbance (254 nm) profile of the analytical-scale reverse-phase HPLC separation of 2.5-L equivalents of residue organics isolated from finished drinking water L...

Figure 5. Direct-acting mutagenic activity of HPLC fractions (1-4, Figure 4) obtained by sequential step elution of residue organics isolated from finished drinking water I. Af/X indicates the mutagenic activity measure for an aliquot of residue organics proportionately reconstituted from the separate fractions Sl/Af is the arithmetic sum of the net revertant colonies for each dose for...

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