Monoclonal antibodies biotinylation

Valdivieso-Garcia, A. Riche, E. Abubakar, O. Waddell, T. E. Brooks, B. W. A double antibody sandwich enzyme-linked immunosorbent assay for the detection of Salmonella using biotinylated monoclonal antibodies. J. Food Prot. 2001, 64,1166-1171. 

Colloidal gold-labeled (strept)avidin can be used as highly sensitive detection reagents for microscopy techniques (Cubie and Norval, 1989) (Chapter 24). Finally, cytotoxic substances coupled to (strept)avidin can be used to direct cell-killing activity toward a tumor-cell-bound, biotinylated monoclonal antibody (or other targeting molecule) for cancer therapy (Hashimoto et al, 1984) (Chapter 21). 

Gretch, D.R., Suter, M., and Stinski, M.F. (1987) The use of biotinylated monoclonal antibodies and streptavidin affinity chromatography to isolate herpesvirus hydrophobic proteins or glycoproteins. Anal. Biochem. 163, 270-277. 

Paganelli, G., Riva, P., Deleide, G., Clivio, A., Chiolerio, F., Scassellati, G.A., Malcovati, M., and Siccardi, A.G. (1988) In vivo labeling of biotinylated monoclonal antibodies by radioactive avidin A strategy to increase tumor radiolocalization. Int.. Cancer 2, 121-125. 

Tse, J. and Goldfarb, S. (1988) Immunohistochemical demonstration of estrophilin in mouse tissues using a biotinylated monoclonal antibody. J. Histochem. Cylochem. 36,1527-1531. 

Aroxmd the same time, Beckman Instruments (now Beckman Coulter) had begun an array-based product development program focused on the use of modified plastics. Silzel and coworkers (1998) and Matson et al. (2001) of Beckman Coulter were among the first to pursue printing of antibodies onto a plastic surface in a microarray format. Silzel et al. immobilized biotinylated monoclonal antibodies onto an avidin-coated polystyrene surface and performed micro-ELISA-based isotyping of IgG species. Matson et al. 

Recently, polyclonal mouse antihuman IgG antibodies fluorescently labeled with Cy5 were used to detect the binding of human myeloma proteins to biotinylated monoclonal antibodies. Again, Cy5-labeled antibodies were used to determine bacterial, viral, and protein antigens bound to biotinylated IgGs. 

Wojda, U. and Miller, J.L. (2000) Targeted transfer of polyethylenimine-avidin-DNA bioconjugates to hematopoietic cells using biotinylated monoclonal antibodies. J. Pharm. Sci., 89, 674-681. 

Add biotinylated monoclonal antibody-streptavidin-HRP, Incubate 30min, 20°C. 

Sung CS, van Osdol WW, Saga T, Neumann RD, Dedrick RL, Weinstein JN. Streptavidin distribution in metastatic tumors pretargeted with a biotinylated monoclonal antibody Theoretical and experimental pharmacokinetics. Cancer Res 1994 54 2166-75. 

Paganelli, G., Magnani, P., Belloni, C., Siccardi, A., and Fazio, F. (1992) Two-step tumor targeting in ovarian cancer patients using biotinylated monoclonal antibodies and radioactive streptavidin. Eur. J. Nucl. Med. 19, 322-329. 

Biotinylated monoclonal antibody to sex hormone binding globulin (SHBG) linked to a gold film treated with (i) chromic acid, (ii) biotin-nitrophenyl ester, and (iii) avidin or strepavidin SHBG [54]... 

Cells are pelleted and resuspended in FACS buffer. Cells are stained with biotinylated monoclonal antibodies (MAb) to non-B-cell markers such as CD4, CDS, GR-1, Mac-1, Terll9, CDllc, and DX5 in FACS buffer see Note 3). 

Vidal, J.C., Bond, L., Duato, P. and Castillo, J.R. (2011) Improved electrochemical competitive immunosensor for ochratoxin A with a biotinylated monoclonal antibody capture probe and colloidal gold nanostructuring. Anal. Methods, 3, 977-984. 

These authors used the same strategy for detecting the CRP. However, in this example a biotinylated RNA aptamer with affinity for CRP was immobilized on the magnetic beads (capture probe) and a biotinylated monoclonal antibody antiCRP conjugated with alkaline phosphatase (AP) (detection probe) was used. The electrochemical detection through DPV allowed a detection limit of 2nM in serum samples (Centi et al., 2009). 

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