Mycobacterium bovis

Tuberculocidal Test. The tubercle bacillus is resistant to disinfectants because the cells are protected with a waxy coating that is not readily penetrated. The tuberculocidal test is a use dilution practical type test that employs porcelain cylinders. The bacteria are different from those in the use dilution method (Table 10), the incubation time is longer, and the details of the procedure are different. For example, in the tuberculocidal test the test is divided into two parts, a presumptive test and a confirmatory test. The former employs Mycobacterium smegmatis and the latter employs Mycobacterium bovis (BCG). For the presumptive test the incubation time is 12 days, as against 48 hours for other bacteria used in the use-dilution method. For the confirmatory test the incubation time is 60 days, with an additional 30 days in case there is no growth. As shown in Table 10, the concentrations of the phenol standard are higher than used with other bacteria. 

Otenhoff, T.H., Kale, A.B., VanEmbden, J.D.A., Thole, J.E.R., Kiessling, R. (1988). The recombinant 65-kD heat shock protein of Mycobacterium bovis bacillus calmette guerin/M. tuberculosis is a target molecule for CD4 + cytotoxic T lymphocytes that lyse human monocytes. J. Exp. Med. 168, 1947-1952. 

Mycobacterium avium Mycobacterium bovis (BCG) Mycobacterium intracellulare Mycobacterium lepraemurium Mycobacterium smegmatis Salmonella typhimurium Candida albicans Cryptococcus neoformans Leishmania donovani Toxoplasma gondii... 

Mycothiol 43 (Scheme 14) is a complex cysteine derivative isolated from Streptomyces clavuligerus, Mycobacterium bovis, and other mycobacteria. It... 

Capreomycin has a pronounced suppressive effect against Mycobacterium tuberculosis and Mycobacterium bovis. Most strains of Mycobacterium kansasii are also sensitive to kanamycin, while other, nontuberculous strains are not sensitive to it. It is often used upon necessity of using parentemal therapy through deep intramuscular injections. Capreomycin is less toxic than kanamycin and has somewhat more of a bacteriostatic effect. Synonyms of this drug are capromycin, capastat, ogostal, and others. 

Bacillus Calmette-Guerin (BCG) is a viable attenuated strain of Mycobacterium bovis. Nonviable strains of the bacterium also have been shown to augment the immune response. The smallest active compound derived from BCG thus far has been identified as muramyl dipeptide. The T cell is a principal target for BCG. It also appears to stimulate natural killer cells, which in turn can kill malignant cells. It has been suggested that BCG cross-reacts immunologically with tumor cell antigens. 

The answer is i. (Katzung, p 984.) Bacille Calmette-Guerin vaccine is a nonspecific stimulant of the reticuloendothelial system. It is an attenuated strain of Mycobacterium bovis that appears most effective in small, localized bladder tumors. This agent is approved for intravesicular use in bladder cancer. Adverse reactions are associated with the renal system, such as problems with urination, infection, and cystitis. 

Tuberculosis is a chronic infectious disease caused by various species of mycobacteria. The important human mycobacterium pathogens are Mycobacterium tuberculosis and Mycobacterium bovis. According to WHO, about one third of the world s popu-... 

BCG, live bacterium Mycobacterium bovis — Bacillus Calmette-Guerin... 

Mycobacterium bovis (bacillus of Calmette and Guerin strain), live [SY] Armand-Frappier strain of BCG [SY]... 

It was not until the late 1960 s that encouraging experimental and clinical results were obtained from the treatment of tumors with bacterial preparations other than endotoxins, primarily with an attenuated antituberculosis vaccine consisting of living bacteria of Mycobacterium bovis strain BCG alone or admixed with tumor cells. However, the use of viable cells of BCG caused complications. 

Kataoka, T., Yamamoto, S., Yamamoto, T., Kuramoto, E., Kimura, Y., Yano, O. and Tokunaga, T. (1992) Antitumor activity of synthetic oligonucleotides with sequences from cDNA encoding proteins of Mycobacterium bovis BCG. Jpn. J. Cancer Res., 83,244-247. 

Tokunaga, T., Yamamoto, H., Shimada, S., Abe, H., Fukuda, T., Fujisawa, Y. et al. (1984) Antitumor activity of deoxyribonucleic acid traction from Mycobacterium bovis BCG. I. Isolation, physicochemical characterization, and antitumor activity. J. Natl. Cancer Inst., 72, 955-962. 

BCG is a viable strain of Mycobacterium bovis that has been used for immunization against tuberculosis. It has also been employed as a nonspecific adjuvant or immunostimulant in cancer therapy but has been successful only in intravesical therapy for superficial bladder cancer. BCG appears to act at least in part via activation of macrophages to make them more effective killer cells in concert with lymphoid cells in the cellular efferent limb of the immune response. Lipid extracts of BCG as well as nonviable preparations of Corynebacterium parvum may have similar nonspecific immunostimulant properties. A chemically defined derivative of the BCG cell wall, [Lys18]-muramyl dipeptide, has been licensed in Japan to enhance bone marrow recovery after cancer chemotherapy. 

Materials. The silver composition of the present invention comprised 10 ppm silver in water. The silver composition was evaluated employing a liquid to liquid matrix against Mycobacterium bovis BCG (TMC 1028). This organism causes tuberculosis in animals and can cause tuberculosis in humans. It is used as a stand-in for M. tuberculosis, the major cause of human tuberculosis, as tests have shown it to have a similar susceptibility to M. tuberculosis. The test organism was exposed to the silver composition in duplicate at four exposure times and quantified using membrane filtration. 

Dong Y, et al. Effect of fluoroquinolone concentration on selection of resistant mutants of Mycobacterium bovis BCG and Staphylococcus aureus. Antimicrob. Agents Chemother., 1999, 43, 1756-1758. 

Tuberculosis (TB) is an infection with mycobacterium causing the presence of tubercles. The most common single source of infection is the lungs. If the tubercles are within the blood and more than two tissues sites (e.g. lungs and liver) then it is called miliary tuberculosis. Tuberculosis is caused by Mycobacterium tuberculosis or more rarely Mycobacterium bovis. Infection spreads by inhalation of infected droplets. 

Molnar, Beladii, and Foldes [69] studied antimycobacterial activity of five phenothiazine derivatives including chlorpromazine, levomeprazine, promazine, promethazine, and diethazine. The growth of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium butyricum was found to be inhibited by chlorpromazine at practically identical concentrations. The minimum inhibitory concentrations for Mycobacterium tuberculosis were chlorpromazine and levomeprazine 10 xg/ml diethazine and promethazine 20 xg/ml whilst chlorpromazine sulphoxide was ineffective even at 100 xg/ml. Chlorpromazine and promethazine exerted a measurable bactericidal activity on Mycobacterium tuberculosis at 50 xg/ml total destruction of the organism and loss of acid fastness in part of the cells were shown at 300 xg/ml. Preliminary studies in mouse experiments revealed that phenothiazine derivatives were ineffective. 

Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis. J. Bacteriol. 178,... 

Spies, H.S.C., and Steenkamp, D.J., 1994, Thiols of intracellular pathogens. Identification of ovothiol A in Leishmania donovani and structural analysis of a novel thiol from Mycobacterium bovis. Eur. J. Biochem. 224 203-213. 

Freund s A mixture of killed/lyophilized Mycobacterium bovis or Mycobacterium... 

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