Mycobacterium smegmatis

Tuberculocidal Test. The tubercle bacillus is resistant to disinfectants because the cells are protected with a waxy coating that is not readily penetrated. The tuberculocidal test is a use dilution practical type test that employs porcelain cylinders. The bacteria are different from those in the use dilution method (Table 10), the incubation time is longer, and the details of the procedure are different. For example, in the tuberculocidal test the test is divided into two parts, a presumptive test and a confirmatory test. The former employs Mycobacterium smegmatis and the latter employs Mycobacterium bovis (BCG). For the presumptive test the incubation time is 12 days, as against 48 hours for other bacteria used in the use-dilution method. For the confirmatory test the incubation time is 60 days, with an additional 30 days in case there is no growth. As shown in Table 10, the concentrations of the phenol standard are higher than used with other bacteria. 

Dioxo-5a-pregnan Cortinarius evernius C-351 Streplomyces griseus4 Mycobacterium smegmatis 30% Penicillium urticae6 9%... 

Purwantini E, L Daniels (1996) Purification of a novel coenzyme F42o-dependent glucose-6-phosphate dehydrogenase from Mycobacterium smegmatis. J Bacteriol 178 2861-2866. 

Mycobacterium avium Mycobacterium bovis (BCG) Mycobacterium intracellulare Mycobacterium lepraemurium Mycobacterium smegmatis Salmonella typhimurium Candida albicans Cryptococcus neoformans Leishmania donovani Toxoplasma gondii... 

Stahl, C., Kubetzko, S., Kaps, I., Seeber, S., Engelhardt, H. and Niederweis, M. (2001). MspA provides the main hydrophilic pathway through the cell wall of Mycobacterium smegmatis, Mol. Microbiol., 40, 451-464. 

Finally, several naturally occurring depsipeptide antibiotics containing the 2,3,4,5-tetrahydropyridazine or the hexahydropyridazine system have to be mentioned. In Japan, antrimycin (132, R = Me) has been isolated from Strepto-myces xanthocidicus MG125-CF1, which in vitro shows antibacterial activity (Mycobacterium smegmatis MIC 12.5 /rg/ml Mycobacterium tuberculosis MIC 50 /rg/ml) [366]. 

In all Mycobacterium spp. two conserved PPTases are found an AcpS that activates two FASs and another PPTase that acts on NRPSs and PKSs that are involved in the biosynthesis of virulence factors. The essential role of both PPTases was demonstrated for the survival of Mycobacterium smegmatis, making them promising targets for the development of new antituberculosis drugs. 

N -Acetylation cleavage to N-acetyl-2-phenylethylamine (Mycobacterium smegmatis) N -Acetylation (Cunninghamella [102] elegans) N -Acetylation cleavage to N-acetyl-amphetamine (Mycobacterium smegmatis)... 

Lechner D, Gibbons S, Bucar F. (2008) Plant phenolic compounds as ethid-ium bromide efflux inhibitors in Mycobacterium smegmatis. J Antimicrob Chemother 62 345-348. 

Iron uptake of Mycobacterium smegmatis involving mycobactin S was studied... 

This group comprises condensation products of salicylic acid with cysteine giving a thiazoline ring. For a review, see (570). Some structurally related compounds will also be mentioned here. Salicylic acid isolated from Burkholderia Pseudomonas) cepacia was named azurochelin (555). It was found to act as a siderophore, e.g. for Pseudomonas fluorescens (230) and P. syringae (178) see also Mycobacterium smegmatis (Sect. 2.8). For details on the siderophore activity of salicylic acid, see (559). 

Ratledge C (1964) Relationship between the Products of Aromatic Biosynthesis in Mycobacterium smegmatis and Aerobacter aerogenes. Nature 203 428... 

Ratledge C, Ewing M (1996) The Occurrence of Carboxymycobactin, the Siderophore of Pathogenic Mycobacteria, as a Second Extracellular Siderophore in Mycobacterium smegmatis. Microbiology 142 2207... 

Ratledge C, Marshall BJ (1972) Iron Transport in Mycobacterium smegmatis the Role of Mycobactin. Biochim Biophys Acta 279 58... 

A number of l-aryl-6-aminouracils (LIX Ar = fluoro- or trifluoromethyl-substituted phenyl, X = H, NO or NHj) were found to possess antiviral activity against rhinovims 1059. Among these, l-(OT-trifluoromethylphenyl)-5,6-diamino-uracil (LIX Ar = W-CF3, X = NH2), possesses antibacterial activity against Bacillus subtilis, Staph, aureus zni Mycobacterium smegmatis [410]. 

Unit, mm B. subtillis, Bacillus subtillis M. smegmatis, Mycobacterium smegmatis. 

For a number of 4,5-dihydro-1,2,4-oxadiazoles, some activity against the fungus Neurospora crassa and against Mycobacterium smegmatis has been observed <87JHC101). The drug imol-amine (260) has a papaverine-like effect on animals and has found application in the treatment of... 

Shankar, S. Hershberger, C.D. Chakrabarty, A.M. The nucleoside diphosphate kinase of Mycobacterium smegmatis identification of proteins that modulate specificity of nucleoside triphosphate synthesis by the enzyme. Mol. Microbiol., 24, 477-487 (1997)... 

Suzuki, H. Kaneko, T. Ikeda, Y. Properties of polyphosphate kinase prepared from mycobacterium smegmatis. Biochim. Biophys. Acta, 268, 381-390 (1972)... 

A low cobalt concentration was shown by Hendlin and Ruger (1950) to limit synthesis of vitamin Bi2. Cobalt comprises about 4% of the molecule. Working with 13 cultures, including a strain of Streptomyces griseus, unidentified rumen and soil isolates, a strain of Mycobacterium smegmatis, and Pseudomonas species, Hendlin and Ruger (1950) found that addition of 1 to 2 ppm of cobalt increased yield by threefold. 

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