MOLT-4 leukemia cell line

The mechanism of inhibition has not been characterized, but it is probably related to the ionophoretic properties of these antibiotics. Monensin has been shown to inhibit the intracellular transport of viral membrane proteins of cells infected with Semliki Forest vims (169). The formation of syncytia, normally observed when T-lymphoblastoid cell line (CEM) cells are cocultivated with human immunodeficiency vims (HlV-l)-infected T-ceU leukemia cell line (MOLT-3) cells, was significantly inhibited in the presence of monensin (170). This observation suggests that the viral glycoproteins in the treated cells were not transported to the cell surface from the Golgi membrane. 

FIGURE 18.9 Concentration-effect curves for the thiopurine analogs, mercaptopurine (MP, open symbols) and thioguanine (TG, closed symbols). Effect is the percentage of cells killed in vitro relative to an untreated control in MOLT 4 (squares), CCRF-CEM (ti iangles), and Wilson (cn des) leukemia cell lines. TG is 10-fold more potent than is MP. (Reproduced with permission from Adamson PC, Poplack DG, Balis EM. Leukemia Res 1994 18 805-10.)... 

Moreover, cyanidin 3-O-rutinoside (5) also induced apoptosis in other human leukemia cell lines and human lymphoma cell lines such as MOLT-4 cells of human acute lymphoblastic leukemia cell line, Daudi cells of human... 

The cytotoxic effects of the synthetic actinocin derivatives were investigated by examination of the drug-induced apoptosis and cell cycle perturbations in a human leukemia MOLT-3 cell line [88]. 

MOLT-3 human T-lineage acute lymphoblastic leukemia cell line... 

Fig. 7 Growth inhibition assay of human Molt-3 leukemia cell line cells were incubated for 96 h. ( ) pro-CPT 35a -l- pro-DOX 34a, ( ) pro-CPT 35a + pro-DOX 34a + 1 jlM 38C2, (A) heterodimeric prodrug 36, (T) heterodimeric prodrug 36 -i-1 jjiM 38C2...

Fig. 10 Growth inhibition assay of the human Molt-3 leukemia cell line, with a fixed concentration of prodrugs and varying concentration of catalytic antibody 38C2. Cells were incubated for 72 h. (o) 36 nM pro-mCPT, ( ) 12 nM pro-tCPT...

Two new cyclic heptapeptides, scytalidamides A 43 and B 44, were isolated from the culture broth of a marine fungus, Scytalidium sp., collected from the Bahamas. Compounds 43 and 44 showed moderate in vitro cytotoxicity toward HCT-116 human colon adenocarcinoma with IC50 values of 2.7 and 11.0 pM, respectively. Both compounds displayed moderate cytotoxicity in the NCI 60 cell-line panel with mean GI50 values of 7.9 and 4.1 pM for 43 and 44, respectively. The most sensitive cell lines were MOLT-4 leukemia (3.0 pM) for 43 and Uacc-257 melanoma (1.2 pM) for 44. The total syntheses of scytalidamide A 43 was achieved on solid phase using two different resins, a phenylalanine silane resin and a 4-methoxybenzaldehyde backbone linker resin. ... 

Knowles and Milner, 2000). Furthermore, these compounds are thought to be involved in the inhibition of certain cytochrome P-450 enzyme-dependent bioactivations of procarcinogens and protoxicants (Brady et al., 1991), as well as to increase levels of glutathione-S-transferase (GST), an enzyme of particular importance in the detoxification of xenobiotics in the body (Wilce and Parker, 1994). Most recently, the ability of various plants and plant extracts to influence apoptosis, or programmed cell death, in cancerous cells in an attempt to arrest their proliferation, has been the topic of much research. Allicin has been shown to induce apoptosis in a variety of cell lines, including human hepatocellular carcinoma cells (KIM-1) and human lymphoid leukemia (MOLT-4B) cells (Thatte et al., 2000). 

It was reported licochalcone A inhibits the cytopathic activity of human immunodeficiency virus (HIV) by Okuda s group [22], A cell line named OKM-1, sensitive to the cytopathic activity of HIV, was established from the peripheral blood of a patient with adult T-cell leukemia. Giant cells due to the cytopathic activity, were formed within a day on co-culture with HIV-infected Molt-4 cell (OKM-1 OKM-4 = 3 1). Licochalcone A inhibited the giant cell formation at concentration 20 ig/ml without observable cytotoxicity. Besides, licochalcone A has a radical scavenging effect, inhibition of leukotriene synthesis in human polymorphonuclear neutrophils and xanthine oxidase. 

A-ACPEA and A-ACTYR were compared for their inhibitory effects on sepiapterin reductase, a catalyzing enzyme for synthesis of tetrahydrobiopterin which is a required cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases. hi MOLT-4 T cell leukemia and MCF-7 breast cancer cell lines, both A-ACPEA and A-ACTYR (Af-acetyl-p-tyramine) showed poor inhibitory effects on sepiapterin reductase, whereas Af-acetyl-m-tyramine was a strong inhibitor [42]. 

Preparation of cell extracts. MOLT 4F is a human T-cell line established from a patient with acute lymphocytic leukemia (13) and has since been maintained in our laboratory. Characteristically, MOLT 4F cells form rosettes with sheep red blood cells (E-rosettes). They do not synthesize immunoglobulin and grow as a single cell suspension. RPMI 1788 is a human B-cell line established from the peripheral blood of a healthy volunteer. These cells produce immunoglobulin, have complement receptors and grow as clusters in suspension culture. Characteristics of these cell lines have recently been summarized by Moore (14). These cells were maintained in medium RPMI 1640 with 10% fetal calf serum and penicillin and streptomycin. The viability of the cells measured by a trypan blue... 

In the trimeric system, one cleavage by the antibody releases three times the amount of CPT than a cleavage in the monomeric prodrug system. We selected one cell line (Molt-3 leukemia) for further studies with fixed con-... 

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