Human leukemia cell line

Nishihara H, Maeda M, Oda A, et al. DOCK2 associates with CrkL and regulates Racl in human leukemia cell lines. Blood 2002 100(12) 3968-3974. 

As for the indole alkaloids harmaline and harmine (Fig. 4), their biosynthesis was stimulated in emhryogenic callus of T. terrestris at concentrations of 66.4 0.5 and 82.7 0.6 /rg/g dw, respectively." Harmaline stimulates the central nervous system while harmine is cytotoxic to human leukemia cell lines HL-60 and K562. 

Civoli F, Daniel LW (1998) Quaternary ammonium analogs of ether lipids inhibit the activation of protein kinase C and the growth of human leukemia cell lines. Cancer Chemother Pharmacol 42 319-326... 

A human leukemia cell line has been used to test 11 anticancer drugs to determine if they are more effective when used alone or in combination. 

A new xanthocillin 80 was isolated from marine fungus Basipetospora sp. as thrombopoietin (TPO) mimics. It promoted the proliferation of a TPO-sensitive human leukemia cell line, UT-7/TPO, and UT-7/EPO-mpl, genetically engineered to express c-Mpl, a receptor for TPO in dose-dependent manners. However, the proliferation of UT-7/EPO, a parental cell line of UT-7/EPO-mpl that was devoid of TPO receptor, was not affected by it. These data indicated that xanthocillin 80 is putative agonists for c-Mpl, as its cellular actions was analogous to those of TPO. 

Fig. 1. Pertussis toxin-mediated ADP ribosylation of membrane G proteins. Isolated cell membranes (50 ng of protein) from N1E 115 cells (mouse neuroblastoma cell line), N2A cells (mouse neuroblastoma cell line), S49-1 eye cells (S49(-) mutated mouse lymphoma cell line deficient in Ga ), 549 wt cells (wild-type mouse lymphoma cell line), RBL (RBL 2H3 rat basophilic leukemia cell line), GH3 cells (GH3 rat hypophyseal tumor cell line), PC-12 (rat pheochromocytoma cell line), HIT-T15 cells (hamster insulinoma cell line), Y-1 cells (mouse adrenal cortex tumor cell line), 108 cc 15 cells (mouse/rat neuroblastoma x glioma hybrid cell line), HL-60 cells (DMSO-differentiated human leukemia cell line), HL-60 (+PT) cells (HL-60 cells pretreated with 25 ng/ml of pertussis toxin for 24 h prior to preparation of membranes), RINm5F cells (rat insulinoma cell line), and C6-2 cells (rat glioma cell line) were subjected to P-ADP-ribosylation as described in section 4.3.3. Samples were precipitated as outlined in section 4.3.5 and subjected to SDS-PAGE with separating gels containing 8% acrylamide (w/v). An autoradiogram of the dried gel is shown. Molecular masses of marker proteins are indicated (kDa). Modified Ga proteins migrate at approximately 40 kDa. Radioactivity running in front of the 30 kDa marker protein comigrates with the dye front...

The isomer trends of the models were very useful for helping the identification of isomers. Cell cultures of human leukemia cell lines (THP-1) were incubated in the absence and presence of thiol compounds, ensuring that no trans compounds could come from the medium [45]. In parallel experiments, some millimolar levels of thiol compounds were added to the cell cultures during incubation, and the comparison of isomeric trends was carried out. A basic content of trans lipids in THP-1 cell membranes could be found during their growth without thiol, and after the addition of the amphiphilic 2-mercaptoethanol, it increased to 5.6% of the main fatty acid residues. Moreover, when a radical stress by y-irradiation is artificially produced in the cell cultures added with thiol, a larger isomerization effect could be seen, with trans lipid formation up to 15.5% in membrane phospholipids. The fatty acid residues most involved in this transformation were arachidonate moieties, as expected. 

Furthermore, the cloned guinea pig liver sigma-1 receptor has been found to contain an endoplasmic reticulum localization motif at the N-terminus (Manner et al., 1996), and antibodies to a human leukemia cell line sigma-1 receptor have localized sigma-1 binding protein to the nuclear membrane of THPl cells (Jbilo et al., 1997). 

Case Study Changes in Potency Caused by Lot-to-Lot Differences in CAMP In this example, THP-1 cells (a human leukemia cell line) were found to be responsive to a biotherapeutic agent in development. The biotherapeutic caused a stimulation of cAMP that accumulated in the cells exposed to drug and could be released after lysing the cells. Therefore, determination of cyclic adenosine monophosphate (cAMP) levels in THP-1 cell culture lysates was used as an indicator... 

TsukadaT, NakashimaK, Shirakawa S. Arachidonate 5-lipoxygenase inhibitors show potent antiproliferative effects on human leukemia cell lines. Biochem Biophys Res Commun. 140 (1986) 832-836. 

Pan et al. have studied the induction of apoptosis signaling pathway by garcinol in human leukemia cell line, HL-60. Their results clearly demonstrate that garcinol strongly induced apoptosis in a dose-dependent manner in HL-60 cells [61]. 

Both compounds display excellent cytotoxicities against a human leukemia cell line, with IC50 values of 3 juM - comparable to doxorubicin. This antitumor activity is attributed to the quinone functionality, as quinones undergo redox cycling via the corresponding hydroquinone to generate reactive oxygen species such as superoxide. The compounds are yet to be synthesized. 

Hosokawa, M., Wanezaki, S., Miyauchi, K., Kurdhara, H., Kohno, H., Kawabata, J., Odashima, S., and Takahashi, K. (1999). Apoptosis inducing effect of fucoxanthin on human leukemia cell line HlL-60. Food Sci. Technol. Res. 5, 243-246. 

M. Nakamura, K. Kirito, J. Yamanoi, T. Wainai, H. Nojiri, and M. Saito, Gang-lioside GM3 can induce megakaryocytoid differentiation of human leukemia cell line K562 cells, Cancer Res., 51 (1991) 1940-1945. 

Human leukemia cell lines Jurkat pharmacologic inhibitors DNA minor groove binding methylsulfonateester lexitropsin [MeOSO(2)(CH(2))(2)-J 58... 

Resveratrol (grape, red wine) HL-60 human leukemia cell line, SHEP neuroblastoma cells Release of cytochrome c from mitochondria, activation of caspases, induction of p53-dependent transcriptional activation sensitizes TRAIL-induced apoptosis decreases in survivin, increases in Smac/DIABLO [155,156]... 

Documented effects Resins from the leaves decrease blood pressure. An infusion of the leaves was shown to slightly reduce blood sugar levels (Gammerman et al. 1990). The flavonoid leachianone G was isolated from the root bark and showed potent antiviral activity against herpes simplex type 1 virus (Du et al. 2003). Two flavonoids isolated from the leaves significantly inhibited the growth of a human leukemia cell line (Kim et al. 2000). Flavonol glycosides, isolated from an extract of the leaves, showed some inhibition of low-density lipoprotein (LDL) oxidation (Katsube et al. 2004, 2006). 

Many isolated steroidal saponins have been shown to be either cytostatic or cytotoxic to HL-60 human leukemia cell lines [43]. Lee et al. reported the in vitro tumor activity of saponins in different cancer cell line and suggested that it could be a good candidate for treatment of pulmonary cancer cells [88]. 

Marigold Tagetes minuta L.) and basil (O. basilicuni) (Lamiaceae) EO showed in vitro cytotoxic effects at concentrations of 25-200 pg/mL. Both EOs reduced the cell viability of HL-60 and NB4 (human leukemia cell line) cells. 82.33% of cell death of HL-60 cells was reached at a concentration of 200 pg/mL basil oil. The highest rate of cell death on NB4 cells (81.87%) was reached with marigold oil at a concentration of 200 pg/mL. Both EOs showed no cytotoxic effects in the in vivo tests (Mahmoud, 2013). 

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