MOLT-4 cells

The 12-membered tetraamine cyclen and bicyclen have inferior anti-HIV activity and are more toxic compared to cyclams and bicyclams. However, Kimura et al. (372) have shown that complexation of the monomeric cyclen (84) to Ni(II), Cu(II), and Zn(II) reduces the toxicity and increases the anti-HIV activity. This is also true for the bicyclen (85), for which the combination of dimerization and metal complexation potentiates the inhibition against HIV-infected MOLT-4 cells (373). 

Two saponins from soybean seeds having soyasapogenol as aglycone were shown to have a partial inhibitory effect on HIV-induced cytopathology in infected human MT-2 lymphocytes cultures [158], The major constituent of group of B saponins from soybean seeds completely inhibited HIV-induced cytophatic effects and virus-specific antigen expression 6 days after infection at concentration > 0.25 mg/ml. Saponins isolated from soybean seeds inhibited HIV-1 replication in MT-4 cells at 0.5 [tg/ml (Nakamura et al. 1992) [159]. These saponins had a narrow therapeutic index and did not inhibit HIV-1 RT. One of them was found to inhibit HIV-induced cell fusion in MOLT-4 cells. 

Cecchinato V, Chiaramonte R, Nizzardo M, Cristofaro B, Basile A, Sherbet GV, Comi P. 2007. Resveratrol-induced apoptosis in human T-cell acute lymphoblastic leukaemia MOLT-4 cells. Biochem Pharmacol 74 1568-1574. 

A natural SP OKU-40 was extracted from the marine microalga Dinoflag-ellata and was found to inhibit the replication of HIV, RSV, influenza A and B viruses, measles virus, and parainfluenza viruses type 2 (PIV-2). However, it did not inhibit the replication of mumps virus or PIV-3 [98]. The action of negatively charged polysaccharides is not merely one of nonspecific inhibition of the binding of an enveloped virus to receptors. In fact, OKU-40 did not inhibit the binding of HIV or influenza A virus to the cell membrane, but it did inhibit the fusion of the membranes of HIV-infected MOLT-4 cells to those of uninfected cells and the fusion of the influenza A virus envelope to uninfected MDCK cells [99]. 

Marui et al. clarified the relation between oxidative stress in the arterial wall (in particular, the endothelium) and the development of the inflammatory response [19,25]. Expression of VCAM-1 by hmnan endothelial cells stimulated by cytokines such as interleukin-1 (IL-1) is mediated by redox-sensitive control mechanisms [25]. The redox-sensitive nature of this gene regulation was determined by the use of antioxidants that are active intracellularly. It was reported that the antioxidant pyrolidine dithiocarbamate (PDTC) inhibited the IL-1-induced endothelial expression of mRNA for VCAM-1 and was as effective as a monoclonal antibody against the VCAM-1 counterligand very late antigen-4 (VLA-4) in inhibiting binding of Molt-4 cells, which express VLA-4 [25]. 

A new cholestane glycoside (18) with a benzoyl-rhamnoglucosyl moiety isolated from Ornithogalum saundersiae strongly inhibited in vitro the growth of human promyelocytic leukemia HL-60 cells and human T-lymphocytes leukemia MOLT-4 cells with IC50 of 21 and 18 nM... 

It was reported licochalcone A inhibits the cytopathic activity of human immunodeficiency virus (HIV) by Okuda s group [22], A cell line named OKM-1, sensitive to the cytopathic activity of HIV, was established from the peripheral blood of a patient with adult T-cell leukemia. Giant cells due to the cytopathic activity, were formed within a day on co-culture with HIV-infected Molt-4 cell (OKM-1 OKM-4 = 3 1). Licochalcone A inhibited the giant cell formation at concentration 20 ig/ml without observable cytotoxicity. Besides, licochalcone A has a radical scavenging effect, inhibition of leukotriene synthesis in human polymorphonuclear neutrophils and xanthine oxidase. 

One downstream effector for ceramide-induced apoptosis may be the retinoblastoma protein (Rb). Serum withdrawal induced cell cycle arrest and dephosphorylation of Rb in MOLT-4 cells associated with increased levels of endogenous cera-mide, whereas sphingosine levels were unaffected. Addition of exogenous ceramide also induced dephosphorylation of Rb in these cells. Ceramide did not induce cell cycle arrest in cells that had defective or inactive Rb (Dbaibo et al., 1995). These data are consistent with the current models of Rb action, in which hypophosphorylation of Rb protein correlates with its ability to bind to transcription factors such as those of of the E2F family and to repress progression through the G1 phase of the cell cycle (Ewen, 1994). 

Cytostatic activities of 43 and some anticancer agents towards HL-60 and MOLT-4 cells... 

Vero monkey kidney (Veto) cells, human T-cell lymphoma Jurkat cells, Molt-4 cells, and freshly cultured human lymphocytes (Begde et al., 2011 Kindrachuk et al., 2013 Murinda et al., 2003). 

Moreover, cyanidin 3-O-rutinoside (5) also induced apoptosis in other human leukemia cell lines and human lymphoma cell lines such as MOLT-4 cells of human acute lymphoblastic leukemia cell line, Daudi cells of human... 

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