Milk-based

Uses of lactose production by appHcation include baby and infant formulations (30%), human food (30%), pharmaceuticals (25%), and fermentation and animal feed (15%) (39). It is used as a diluent in tablets and capsules to correct the balance between carbohydrate and proteins in cow-milk-based breast milk replacers, and to increase osmotic property or viscosity without adding excessive sweetness. It has also been used as a carrier for flavorings. 

Milk. Imitation milks fall into three broad categories filled products based on skim milk, buttermilk, whey, or combinations of these synthetic milks based on soybean products and toned milk based on the combination of soy or groundnut (peanut) protein with animal milk. Few caseinate-based products have been marketed (1,22,23). Milk is the one area where nutrition is of primary concern, especially in the diets of the young. Substitute milks are being made for human and animal markets. In the latter area, the emphasis is for products to serve as milk replacers for calves. The composition of milk and filled-milk products based on skim milk can be found in Table 10. Table 15 gives the composition of a whey /huttermilk-solids-hased calf-milk replacer, which contains carboxymethyl cellulose (CMC) for proper viscosity of the product. 

Products other than filled milk based on caseinate. Corrected to same fat and protein level as dairy product. 

A. Abdullah, A.V.A Resurreccion and L.R. Beuchat, Formulation and evaluation of a peanut milk based whipped topping using response surface methodology. Lebensm. Wiss. Technol., 26 (1993) 162-166. 

There is no experimental evidence available to assess whether the toxicokinetics of -hexane differ between children and adults. Experiments in the rat model comparing kinetic parameters in weanling and mature animals after exposure to -hexane would be useful. These experiments should be designed to determine the concentration-time dependence (area under the curve) for blood levels of the neurotoxic /7-hcxane metabolite 2,5-hexanedione. w-Hcxanc and its metabolites cross the placenta in the rat (Bus et al. 1979) however, no preferential distribution to the fetus was observed. -Hexane has been detected, but not quantified, in human breast milk (Pellizzari et al. 1982), and a milk/blood partition coefficient of 2.10 has been determined experimentally in humans (Fisher et al. 1997). However, no pharmacokinetic experiments are available to confirm that -hexane or its metabolites are actually transferred to breast milk. Based on studies in humans, it appears unlikely that significant amounts of -hexane would be stored in human tissues at likely levels of exposure, so it is unlikely that maternal stores would be released upon pregnancy or lactation. A PBPK model is available for the transfer of M-hcxanc from milk to a nursing infant (Fisher et al. 1997) the model predicted that -hcxane intake by a nursing infant whose mother was exposed to 50 ppm at work would be well below the EPA advisory level for a 10-kg infant. However, this model cannot be validated without data on -hexane content in milk under known exposure conditions. 

Van Staden reported a rapid, reliable automated method for direct measurement of the chloride content in milk based on the principles of flow injection analysis and the use of a dialyser to remove interferents. Dialysed chloride was measured by means of a coated tubular chloride ion-selective electrode. Potential changes arising from the interference of casein were thus avoided and baseline stability ensured. The results obtained for chloride in milk compared well with those provided by standard recommended methods. The linear range for chloride was 250-5000 pg/mL for 30 pL of sample, and the coefficient of variation was better than 0.5%. The throughput was ca. 120 samples/h [132],... 

Compatible with protein-rich beverages (e.g., milk-based soy-based) treated with high-temperature short-time (HTST) pasteurization. However, may be issues with flocculation, thickening, or sedimentation due to Ca-protein interactions when subjected to ultra-high temperature (UHT) heat treatment... 

Pointillart, A., and Gueguen, L. (1993). Calcium bioavailability in pig fed a dry milk-based diet versus a standard diet containing Ca citrate malate. In "BioavailabiUty 93 Nutritional, Chemical and Food Processing Implications of Nutrient Availability". (U. Schlemmer, ed.), pp. 1-5. Ettlingen, May 9-12 [Proceedings]. 

Concerning food peptides, most of the HPLC applications deal with food characterization (based on peptide profile), peptide separation, and identification [172,173] and detection of frauds in milk-based products, meat, and protein hydrolysates [115],... 

Determination of vitamin A and E in infant milk-based formulae Determination of carotenoids in pumpkins... 

Among the vitamin K vitamers, only phylloquinone is accounted for routine food analysis. Furthermore infant formulas, both milk-based and soy protein-based, are supplemented with a synthetic preparation of phylloquinone (the only form admitted) [403], which usually contain about 10% of the biologically inactive ctT-isomer [497]. 

Milk is probably the most adaptable and flexible of all food materials, as will be apparent from Table 1.7, which shows the principal families of milk-based foods - some of these families contain several hundred different products. 

The use of luciferase to quantify ATP (Blum and Coulet, 1994) in milk is the principle of modern rapid methods for assessing the bacteriological quality of milk based on the production of ATP by bacteria. Such methods have been automated and mechanized. 

Equations have been developed to estimate the total solids content of milk based on % fat and specific gravity (usually estimated using a lactometer). Such equations are empirical and suffer from a number of drawbacks for further discussion see Jenness and Patton (1959). The principal problem is the fact that the coefficient of expansion of milk fat is high and it contracts slowly on cooling and therefore the density of milk fat (Chapter 3) is not constant. Variations in the composition of milk fat and in the proportions of other milk constitiuents have less influence on these equations than the physical state of the fat. 

Differential Solubility Methods. Numerous methods have been developed to obtain one or more of the various caseins from whole casein or directly from skim milk based on their differential solubility (Thompson 1971 Mackinlay and Wake 1971 Whitney 1977). While some early procedures indicated the possibility of fractionating whole casein into different components, it was not until the 1950s that systematic procedures were proposed for the fractionation of casein into Warner s a-, 0-, and y-caseins. Hipp et al. (1952) developed two procedures which have been used extensively or partially incorporated into other methods. The first is based upon the differential solubilities of the caseins in 50% alcohol in the presence of ammonium acetate by varying the pH, temperature, and ionic strength. The second procedure involves the dispersion of whole casein in 6.6 M urea and the separa-... 

Another sensitive colorimetric procedure is that of Mackenzie et al (1967), which utilizes the dye Rhodamine B to form benzene-soluble complexes with fatty acids. Nakai et al. (1970) developed a rapid, simple method for screening rancid milk based on the foregoing procedure. The test is said to detect rancid milk with an ADV above 1.2. Like the copper or cobalt soap method, the Rhodamine B reagent is also limited to the longer-chain fatty acids. Kason et al (1972) used the method employing Rhodamine 6G of Chakrabarty et al (1969) to investigate... 

Currently, high-performance liquid chromatography (HPLC) methods have been widely used in the analysis of tocopherols and tocotrienols in food and nutrition areas. Each form of tocopherol and tocotrienol can be separated and quantified individually using HPLC with either a UV or fluorescence detector. The interferences are largely reduced after separation by HPLC. Therefore, the sensitivity and specificity of HPLC methods are much higher than those obtained with the colorimetric, polarimetric, and GC methods. Also, sample preparation in the HPLC methods is simpler and more efficiently duplicated than in the older methods. Many HPLC methods for the quantification of tocopherols and tocotrienols in various foods and biological samples have been reported. Method number 992.03 of the AOAC International Official Methods of Analysis provides an HPLC method to determine vitamin E in milk-based infant formula. It could probably be said that HPLC methods have become dominant in the analysis of tocopherols and tocotrienols. Therefore, the analytical protocols for tocopherols and tocotrienols in this unit are focused on HPLC methods. Normal and reversed-phase HPLC methods are discussed in the separation and quantification of tocopherols and tocotrienols (see Basic Protocol). Sample... 

Chase, G.W. Jr. and Long, A.R. 1998. Liquid chromatographic method for analysis for all-rac-al-pha-tocopheryl acetate and retinyl palmitate in milk-based infant formula using matrix solid-phase dispersion. J. AOAC Int. 81 582-586. 

Tanner, J.T., Barnett, S.A., and Mountford, M.K. 1993. Analysis of milk based infant formula. Phase V. Vitamins A and E, folic acid, and pantothenic acid Food and Dairy Administration-Infant Formula Council Collaborative study. J. AOAC Int. 76 399-401. 

Quantification of the degradation products of raw-milk proteins by HPLC may furnish information on the shelf life of subsequently prepared UHT milk. Based on two earlier methods, Mottar et al. (127) applied HPLC to determine the specific proteolytic components that provide information concerning the presence and activity of gram-negative psychrotrophic bacteria. 

In the latest (1995) edition of Official Methods of Analysis of AOAC International, HPLC methods have been introduced for the first time for the determination of vitamin A in milk (55) and vitamins A (56), D3 (57), E (58), and K, (59) in milk-based infant formulas. 

AOAC official method 992.04. Vitamin A (retinol isomers) in milk and milk-based infant formula. Liquid chromatographic method. In MP Bueno, ed. Official Methods of Analysis of AOAC International. 16th ed. Arlington, VA AOAC International, 1995, p. 50-1-50-2. 

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