Mass spectrometry detecting

GAS CHROMATOGRAPHY/MASS SPECTROMETRY DETECTION OF XENOBIOTICS IN ENVIRONMENTAL... 

Sample concentration, and hence enrichment, is certainly a key issue in this area of analysis, since complementary information obtained from NMR or IR spectroscopic detection is often desirable in conjunction with mass spectrometry. Detection methods such as these have far higher concentration thresholds than MS and obtaining adequate quantities of material for detection becomes a significant challenge. 

Vieno NM, Tuhkanen T, Kronberg L (2006) Analysis of neutral and basic pharmaceuticals in sewage treatment plants and in recipient rivers using solid phase extraction and liquid chromatography-tandem mass spectrometry detection. J Chromatogr A 1134 101-111... 

The choice of the method of analysis depends on the question to address. Spectrophotometry is sufficient for total curcuminoid content determination in a turmeric extract. Separation techniques coupled to mass spectrometry detection and MALDl-TOF are highly sensitive techniques that are more adapted to the identification of metabolites in biological fluids such as urine or plasma. ... 

R. Trones, Development of Packed Capillary High Temperature Liquid Chromatography Utilizing Light Scattering and Inductively Coupled Plasma Mass Spectrometry Detection, PhD. Thesis, University of Oslo (1999). 

Coldham, N. G. Woodward, M. J. Characterization of the Salmonella typhimurium proteome by semi-automated two dimensional HPLC-mass spectrometry Detection of proteins implicated in multiple antibiotic resistance. J. Proteome Res. 2004, 3,595-603. 

Further developments of this topic are in progress, both in developing theoretical models and in applying the procedures to real experimental cases. In particular, the methods can be extended to hyphenated techniques to investigate the complex signals obtained from mass spectrometry detection (Pietrogrande et al., 2006b). 

Liquid chromatography combined with mass spectrometry detection... 

Aguilar C, Borrull F, Marce RM (1997) Determination of pesticides in environmental waters by solid-phase extraction and gas chromatography with electron-capture and mass spectrometry detection. J Chromatogr A 771(1-2) 221-231... 

Mass spectrometry detection gained the acceptance of bioanalytical scientists primarily based on its higher selectivity compared to detection that relies on UV/visible absorbance. Absorption spectra of aqueous solutions usually appear as broad absorbance bands. The selectivity provided by UV/visible absorbance for a colorless analyte is usually very low. To detect a colorless analyte, a wavelength setting below 210 nm is usually used. UV absorbance in this region is not specific because most compounds containing hetero-atoms and multiple bonds absorb UV below 200 to 210 nm. 

Gimeno R.A. et al., 2002. Determination of polycyclic aromatic hydrocarbons and polycyclic aromatic sulfur heterocycles by high-performance liquid chromatography with fluorescence and atmospheric pressure chemical ionization mass spectrometry detection in seawater and sediment samples. J Chromatogr A 958 141. 

Today s personalized medicine requires analysis of a large number of biological samples in a short period on the day they are collected from patients so that a proper informed dose adjustment can be made before subsequent dosing. The high-throughput analytical procedures developed to meet this demand are reviewed in subsequent sections covering immunoassays, HPLC alone and combined with tandem mass spectrometry detection (HPLC-MS/MS), and ultra-performance liquid chromatography with MS/MS detection (UPLC-MS/MS). 

In this regard several sophisticated chromatographic methods, with a quantification limit down to about 0.2 ng/g, have been developed and published for the determination of zearalenone. The methods were mainly based on high-performance liquid chromatography (HPLC) with fluorescence detection (Krska 1998 Visconti and Pascale 1998 Schuhmacher et al. 1998 Tanaka et al. 2000), but HPLC with mass spectrometry detection was also used (Shirai et al. 2000 Josephs et al. 2001). 

M.-R. Fuh and K.-J. Chia, Determination of sulphonated azo dyes in food by ion-pair liquid chromatography with photodiode array and electrospray mass spectrometry detection. Talanta, 56 (2002) 663-671. 

Cabrera and co-workers [28] reported the first combinations of monolithic columns (50 mm x 4.6 mm and 100 mm x 4.6 mm) and mass spectrometry detection, a novel system configuration able to demonstrate a doubling of throughput by using increased flow rates while also maintaining separation efficiency. 

Henchiz Y. Guillarme D. Martel S. Rudaz S. Veuthey J-L. Carrupt P-A. Fast log P determination by ultra-high-pressure liquid chromatography coupled with UV and mass spectrometry detections. Analytical andBioanalytical Chemistry, 2009, 394, 1919-1630. 

Liu H, Sabus C, Carter GT, Du C, Avdeef A, Tischler M (2003) In vitro permeability of poorly aqueous soluble compounds using different solubilizers in the PAMPA assay with liquid chromatography/mass spectrometry detection. Pharm Res 20 1820-1826. 

Test methods that analyze individual compounds (e.g., benzene-toluene-ethylbenzene-xylene mixtures and PAHs) are generally applied to detect the presence of an additive or to provide concentration data needed to estimate environmental and health risks that are associated with individual compounds. Common constituent measurement techniques include gas chromatography with second-column confirmation, gas chromatography with multiple selective detectors, and gas chromatography with mass spectrometry detection (GC/MS) (EPA 8240). 

Peak purity tests are used to demonstrate that an observed chromatographic peak is attributable to a single component. Mass spectrometry is the most sensitive and accurate technique to use for peak purity evaluation because of the specific information derived from the analysis. However, a good number of HPLC methods use mobile phase conditions that are incompatible with mass spectrometry detection. In this case, PDA spectrophotometers using peak purity algorithms may be used to support the specificity of the method. Almost all commercially available diode array detectors are equipped with proprietary software that will perform these calculations. Although this technique is more universal in application to HPLC methods, the data provided is neither particularly... 

Stutz, H. (2005). Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix-assisted laser desorption/ionization and electrospray—mass spectrometry detection. Electrophoresis 26, 1254—1290. 

Several publications describe electrochemical detection techniques, and some authors report indirect fluorescence detection and mass spectrometry detection. 

Boatto, G., Nieddu, M., Carta, A., Pau, A., Palomba, M., Asproni, B., and Cerri, R. (2005). Determination of amphetamine-derived designer drugs in human urine by SPE extraction and capillary electrophoresis with mass spectrometry detection.. Chromatogr. B 814, 93—98. 

Lazar, I. M., Ramsey, R. S., Sundberg, S., and Ramsey, J. M. (1999). Subattomole-sensitivity microchip nanoelectrospray source with tlme-of-flight mass spectrometry detection. Anal. Chem. 71, 3627-3631. 

Andersen M.D., Shaffer J., Jennings P.A., Adams J.A. Structural characterization of protein kinase A as a function of nucleotide binding. Hydrogen-deuterium exchange studies using matrix-assisted laser desorption ionization-time of flight mass spectrometry detection. J. Biol. Chem. 2001, 276, 14204-14211. 

Postigo C, de Alda MIL, Barcelo D et al (2010) Analysis and occurrence of selected medium to highly polar pesticides in groundwater of Catalonia (NE Spain) an approach based on on-line solid phase extraction-liquid chromatography-electrospray-tandem mass spectrometry detection. J Hydrol 383 83-92... 

Damsten, M.C., Commandeur, J.N.M., Fidder, A., Hulst, A.G.,Touw, D., Noort, D. and Vermeulen, N.P.E. (2007) Liquid chromatography/tandem mass spectrometry detection of covalent binding of acetaminophen to human serum albumin. Drug Metabolism and Disposition The Biological Fate of Chemicals, 35 (8), 1408-1417. 

Nicoletti I, De Rossi A, Giovinazzo G, Corradini D. Identification and quantification of stUbenes in fruits of transgenic tomato plants (Lycopersicon esculentum Mill.) by reversed phase HPLC with photodiode array and mass spectrometry detection. Journal of Agricultural and Food Chemistry 55, 3304-3311, 2007. 

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